NCBI PubMed ID: 15013402
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: malcolm.perry
nrc-cnrc.gc.caInstitutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A OR6, Department of Virology, Immunology and Medical Genetics, Department of Medicine, Division of Infectious Diseases, The Center for Microbial Interface Biology, The Ohio State University, Columbus, OH, USA
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, (1)H and (13)C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-d-galacturonamide (d-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-d-glucose (d-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure: [Formula: see text] With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common -->4)-d-GalpNAcAN-(1-->4)-alpha-d-GalpNAcAN-(1--> disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species
lipopolysaccharide, NMR, O antigen, O-antigen, structure, common, tetrasaccharide, characterization, antigenic, polymer, Research, antibodies, antibody, species, component, O-polysaccharide, O antigens, O polysaccharide, O-antigens, bacteria, neutral, biological, serological, serology, chemical, disaccharide, MS, linear, occurrence, lipopolysaccharide O-antigen, virulent, cross-reactivity, crossreactivity, PDF, murine
Structure type: chemical repeating unit of polymerLocation inside paper: Fig. 4
Compound class: O-polysaccharide, O-antigen
Methods: NMR-2D, methylation, NMR, sugar analysis, MS
Biological activity: serological studies
Related record ID(s): 1613, 9246, 9363, 9365, 115176
NCBI Taxonomy refs (TaxIDs): 287
Reference(s) to other database(s): GlycomeDB:5614