| Enzyme / Gene |
Activity |
Object |
References |
Enzyme name: csh1p
UniProt ID: P38287*
CAZy family: GT32
Gene name: CSH1
Gene GenBank ID: 852458* | Synthesized dimer: DManp(1-2)INO
INO)
Donor (ID 34509): ?DManp(1-P-P-5)xXnucG
Acceptor (ID 34606): l?2HOCrt(1-2)[INO(1-P-1)]xXphSphC18
Status: semi-direct evidence in vivo
Confirmation methods: in silico; in vitro (membrane preparation); double mutation; supplementation
ID: 1 Notes: Unlike Dcsg1 cells, the Dcsh1 mutant produced IPC-C (or IPC-D) and mannosylated IPC species at ratios similar to those in wild-type cells. In the Dcsg1Dcsh1 double mutant, however, production of MIPC and M(IP)2C was completely abolished
| Organism (ID 10157,10896,10898,16655,16656,16657,16658,16659): Saccharomyces cerevisiae SEY6210, KA31-1A, SUY41, EHY227, KHY195, SUY45, SUY49, SUY53
Full structure (ID 34607):
[?DManp(1-2)INO(1-P-1)]xXphSphC18&highlight=3~2~?DMan) CSDB ID(s): 43539, 45004, 45512Molecule role (precursor of): glycolipid | Lisman et al. 2004
DOI: 10.1074/jbc.M306119200
Uemura et al. 2003
DOI: 10.1074/jbc.M305498200
Beeler et al. 1997
DOI: 10.1007/s004380050530
Tanaka et al. 2018
DOI: 10.1111/febs.14509
Morimoto et al. 2015
DOI: 10.1111/mmi.12896
|
Enzyme name: sur1p
UniProt ID: P33300*
CAZy family: GT32
Gene name: SUR1/CSG1/BCL21
Gene GenBank ID: 856050* | Synthesized dimer: DManp(1-2)INO
INO)
Donor (ID 34509): ?DManp(1-P-P-5)xXnucG
Acceptor (ID 34606): l?2HOCrt(1-2)[INO(1-P-1)]xXphSphC18
Status: indirect evidence in vivo
Confirmation methods: mutation
ID: 3 Notes: SUR1/CSG1 is both genetically and biochemically related to CSG2. The csg1 mutant produced greatly reduced levels of the mannosylated sphingolipids MIPC and M(IP)2C, and accumulated IPC-C and IPC-D; note that IPC-C contains a monohydroxylated C26 fatty acid whereas the C26 fatty acid in IPC-D is dihydroxylated.
| Organism (ID 16660,16661): Saccharomyces cerevisiae DBY689, CuH3
Full structure (ID 34607):
[?DManp(1-2)INO(1-P-1)]xXphSphC18&highlight=3~2~?DMan) CSDB ID(s): 43539, 45004, 45512Molecule role (precursor of): glycolipid |
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
1 csh1p P38287 * GT32 CSH1 852458 * 34607 l?2HOCrt(1-2)[?DManp(1-2)INO(1-P-1)]xXphSphC18 0 ?DManp(1-2)INO 3~2 ?DManp(1-P-P-5)xXnucG l?2HOCrt(1-2)[INO(1-P-1)]xXphSphC18 semi-direct in silico; in vitro (membrane preparation); double mutation; supplementation Unlike Dcsg1 cells, the Dcsh1 mutant produced IPC-C (or IPC-D) and mannosylated IPC species at ratios similar to those in wild-type cells. In the Dcsg1Dcsh1 double mutant, however, production of MIPC and M(IP)2C was completely abolished glycolipid 0 1 43539 45004,45512 10157,10896,10898,16655,16656,16657,16658,16659 Saccharomyces cerevisiae SEY6210, KA31-1A, SUY41, EHY227, KHY195, SUY45, SUY49, SUY53 Lisman et al. 2004 (doi: 10.1074/jbc.M306119200); Uemura et al. 2003 (doi: 10.1074/jbc.M305498200); Beeler et al. 1997 (doi: 10.1007/s004380050530); Tanaka et al. 2018 (doi: 10.1111/febs.14509); Morimoto et al. 2015 (doi: 10.1111/mmi.12896)
3 sur1p P33300 * GT32 SUR1/CSG1/BCL21 856050 * 34607 l?2HOCrt(1-2)[?DManp(1-2)INO(1-P-1)]xXphSphC18 0 ?DManp(1-2)INO 3~2 ?DManp(1-P-P-5)xXnucG l?2HOCrt(1-2)[INO(1-P-1)]xXphSphC18 indirect mutation SUR1/CSG1 is both genetically and biochemically related to CSG2. The csg1 mutant produced greatly reduced levels of the mannosylated sphingolipids MIPC and M(IP)2C, and accumulated IPC-C and IPC-D; note that IPC-C contains a monohydroxylated C26 fatty acid whereas the C26 fatty acid in IPC-D is dihydroxylated. glycolipid 0 1 43539 45004,45512 16660,16661 Saccharomyces cerevisiae DBY689, CuH3 Lisman et al. 2004 (doi: 10.1074/jbc.M306119200); Uemura et al. 2003 (doi: 10.1074/jbc.M305498200); Beeler et al. 1997 (doi: 10.1007/s004380050530); Tanaka et al. 2018 (doi: 10.1111/febs.14509); Morimoto et al. 2015 (doi: 10.1111/mmi.12896)