| Enzyme / Gene |
Activity |
Object |
References |
Enzyme name: 81A1
UniProt ID: O81770.1
CAZy family: GT28
Gene name: UGT81A1 / MGD1 / AT4G31780
Gene GenBank ID: 829306 | Synthesized dimer: bDGalp(1-1)DGro
xDGro?)
Donor (ID 33991): ?DGalp(1-P-P-5)xXnucU
Acceptor (ID 33992): LIP(1-2)[LIP(1-3)]xDGro
Status: direct evidence in vivo
Confirmation methods: in vitro (crude extract), expression in E.coli (as whole-cell biocatalyst), mutation
ID: 1547 Notes: Possible LIP = hexadecatrienoic, alpha-linolenic, trans-delta3-hexadecenoic acids
| Organism (ID 9805): Arabidopsis thaliana
Expressed in: E. coli; flower, leaf, stem, root, silique, seed
Full structure (ID 28317):
[LIP(1-3),LIP(1-2)]xDGro&highlight=1~1~bDGal) CSDB ID(s): 60937, 60939, 61034, 61035, 61038, 66724 | Jorasch et al. 2000
DOI: 10.1046/j.1432-1327.2000.01414.x
Kobayashi et al. 2004
DOI: 10.1104/pp.103.032656
Kobayashi et al. 2007
DOI: 10.1073/pnas.0704680104
Awai et al. 2001
DOI: 10.1073/pnas.181331498
Fujii et al. 2014
DOI: 10.1104/pp.114.250050
Rocha et al. 2013
DOI: 10.1016/j.biochi.2012.11.011
|
Synthesized dimer: bDGalp(1-1)DGro
xDGro?)
Donor (ID 33991): ?DGalp(1-P-P-5)xXnucU
Acceptor (ID 33995): lXPam(1-2)[lXOle(1-3)]xDGro
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 1549 | Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: flower, leaf, stem, root, silique, seed
Full structure (ID 33996):
[lXPam(1-3),lXOle(1-2)]xDGro&highlight=1~1~bDGal) | Kobayashi et al. 2004
DOI: 10.1104/pp.103.032656
Kobayashi et al. 2007
DOI: 10.1073/pnas.0704680104
Awai et al. 2001
DOI: 10.1073/pnas.181331498
|
Synthesized dimer: bDGalp(1-1)DGro
xDGro?)
Donor (ID 33991): ?DGalp(1-P-P-5)xXnucU
Acceptor (ID 33997): lXLin(1-2)[lXLin(1-3)]xDGro
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 1550 | Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: flower, leaf, stem, root, silique, seed
Full structure (ID 20118):
[lXLin(1-3),lXLin(1-2)]xDGro&highlight=1~1~bDGal) CSDB ID(s): 47110 |
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
1547 81A1 O81770.1 GT28 UGT81A1 / MGD1 / AT4G31780 829306 28317 bDGalp(1-1)[LIP(1-3),LIP(1-2)]xDGro 0 bDGalp(1-1)xDGro? 1~1 ?DGalp(1-P-P-5)xXnucU LIP(1-2)[LIP(1-3)]xDGro in vivo in vitro (crude extract), expression in E.coli (as whole-cell biocatalyst), mutation Possible LIP = hexadecatrienoic, alpha-linolenic, trans-delta3-hexadecenoic acids 0 0 60937,60939,61034,61035,61038,66724 9805 Arabidopsis thaliana E. coli; flower, leaf, stem, root, silique, seed Jorasch et al. 2000 (doi: 10.1046/j.1432-1327.2000.01414.x); Kobayashi et al. 2004 (doi: 10.1104/pp.103.032656); Kobayashi et al. 2007 (doi: 10.1073/pnas.0704680104); Awai et al. 2001 (doi: 10.1073/pnas.181331498); Fujii et al. 2014 (doi: 10.1104/pp.114.250050); Rocha et al. 2013 (doi: 10.1016/j.biochi.2012.11.011)
1549 81A1 O81770.1 GT28 UGT81A1 / MGD1 / AT4G31780 829306 33996 bDGalp(1-1)[lXPam(1-3),lXOle(1-2)]xDGro 0 bDGalp(1-1)xDGro? 1~1 ?DGalp(1-P-P-5)xXnucU lXPam(1-2)[lXOle(1-3)]xDGro in vitro in vitro (purified protein) 0 0 9805 Arabidopsis thaliana flower, leaf, stem, root, silique, seed Kobayashi et al. 2004 (doi: 10.1104/pp.103.032656); Kobayashi et al. 2007 (doi: 10.1073/pnas.0704680104); Awai et al. 2001 (doi: 10.1073/pnas.181331498)
1550 81A1 O81770.1 GT28 UGT81A1 / MGD1 / AT4G31780 829306 20118 bDGalp(1-1)[lXLin(1-3),lXLin(1-2)]xDGro 0 bDGalp(1-1)xDGro? 1~1 ?DGalp(1-P-P-5)xXnucU lXLin(1-2)[lXLin(1-3)]xDGro in vitro in vitro (purified protein) 0 0 47110 9805 Arabidopsis thaliana flower, leaf, stem, root, silique, seed Kobayashi et al. 2004 (doi: 10.1104/pp.103.032656); Kobayashi et al. 2007 (doi: 10.1073/pnas.0704680104); Awai et al. 2001 (doi: 10.1073/pnas.181331498)