| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: 89C1
UniProt ID: Q9LNE6.1 
CAZy family: GT1
Gene name: AT1G06000
Gene GenBank ID: 837109 | Synthesized dimer: aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13
Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13)
Donor (ID 33866): ?LRhap(1-P-P-5)xXnucU
Acceptor (ID 33672): Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13
Status: evidence in vitro
Confirmation methods: expression in E.coli (as whole-cell biocatalyst)
ID: 1673 Notes: 89C1 demonstrates this activity when coexpressed with UDP-L-rhamnose synthase AtRHM1,
which catalyzes the synthesis of UDP-L-rhamnose from UDP-D-glucose; presumably,
89C1 can use pools of both TDP-L-rhamnose and UDP-L-rhamnose present in such
recombinant bacteria.
| Organism (ID 9805): Arabidopsis thaliana
Expressed in: E. coli; leaf, stem, root, flower bud, flower
Full structure (ID 34035):
Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13&highlight=1~7~aLRha) | Yonekura-Sakakibara et al. 2007
DOI: 10.1074/jbc.M611498200
Parajuli et al. 2015
DOI: 10.1016/j.carres.2015.09.010
|
Synthesized dimer: aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13
Donor (ID 33935): ?LRhap(1-P-P-5)xXnucT
Acceptor (ID 33672): Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13
Status: evidence in vitro
Confirmation methods: expression in E.coli (as whole-cell biocatalyst)
ID: 1674 | Organism (ID 9805): Arabidopsis thaliana
Expressed in: E. coli; leaf, stem, root, flower bud, flower
Full structure (ID 34035):
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
1673 89C1 Q9LNE6.1 GT1 AT1G06000 837109 34035 aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 0 aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 1~7 ?LRhap(1-P-P-5)xXnucU Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 in vitro expression in E.coli (as whole-cell biocatalyst) 89C1 demonstrates this activity when coexpressed with UDP-L-rhamnose synthase AtRHM1, which catalyzes the synthesis of UDP-L-rhamnose from UDP-D-glucose; presumably, 89C1 can use pools of both TDP-L-rhamnose and UDP-L-rhamnose present in such recombinant bacteria. 0 0 9805 Arabidopsis thaliana E. coli; leaf, stem, root, flower bud, flower Yonekura-Sakakibara et al. 2007 (doi: 10.1074/jbc.M611498200); Parajuli et al. 2015 (doi: 10.1016/j.carres.2015.09.010)
1674 89C1 Q9LNE6.1 GT1 AT1G06000 837109 34035 aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 0 aLRhap(1-7)Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 1~7 ?LRhap(1-P-P-5)xXnucT Subst // Subst = naringenin = SMILES O=C1CC(C2=CC={54}C(O)C=C2)OC3=C{7}C(O)={6}C{5}C(O)=C13 in vitro expression in E.coli (as whole-cell biocatalyst) 0 0 9805 Arabidopsis thaliana E. coli; leaf, stem, root, flower bud, flower Yonekura-Sakakibara et al. 2007 (doi: 10.1074/jbc.M611498200); Parajuli et al. 2015 (doi: 10.1016/j.carres.2015.09.010)