| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: AtFUT11
UniProt ID: Q9LJK1.1 
CAZy family: GT10
Gene name: FUT11 / FucTA / AT3G19280
Gene GenBank ID: 821462 | Synthesized dimer: aLFucp(1-3)bDGlcpN1N
bDGlcpN1N)
Donor (ID 34115): ?LFucp(1-P-P-5)xXnucG
Acceptor (ID 34091): Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn
Status: direct evidence in vivo
Confirmation methods: in vitro (crude extract), mutation
ID: 1735 Notes: Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN
in
planta.
| Organism (ID 9805): Arabidopsis thaliana
Full structure (ID 34118):
[Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn&highlight=2~3~aLFuc) | Strasser et al. 2004
DOI: 10.1016/S0014-5793(04)00150-4
Wilson et al. 2001
DOI: 10.1016/S0304-4165(01)00151-9
|
Synthesized dimer: aLFucp(1-3)bDGlcpN1N
Donor (ID 34115): ?LFucp(1-P-P-5)xXnucG
Acceptor (ID 34119): Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[aLFucp(1-6),Ac(1-2)]bDGlcpN1N(1-4)x?Asn
Status: direct evidence in vivo
Confirmation methods: in vitro (purified protein), mutation
ID: 1736 Notes: Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN
in
planta.
| Organism (ID 9805): Arabidopsis thaliana
Full structure (ID 34120):
[aLFucp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn&highlight=2~3~aLFuc) | Yoo et al. 2015
DOI: 10.1074/jbc.M115.653162
Strasser et al. 2004
DOI: 10.1016/S0014-5793(04)00150-4
Both et al. 2011
DOI: 10.1093/glycob/cwr056
|
Synthesized dimer: aLFucp(1-3)bDGlcpN1N
Donor (ID 34115): ?LFucp(1-P-P-5)xXnucG
Acceptor (ID 34121): bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn
Status: indirect evidence in vivo
Confirmation methods: mutation
ID: 1737 Notes: Suggested activity in planta. Both AtFUT11 and AtFUT12 are required for synthesis
of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN
in
planta.
| Organism (ID 9805): Arabidopsis thaliana
Full structure (ID 34122):
[bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn&highlight=2~3~aLFuc) | Strasser et al. 2004
DOI: 10.1016/S0014-5793(04)00150-4
Yoo et al. 2015
DOI: 10.1074/jbc.M115.653162
|
Synthesized dimer: aLFucp(1-3)bDGlcpN1N
Donor (ID 34123): ?LGalp(1-P-P-5)xXnucG
Acceptor (ID 34124): bDManp(1-?)bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 1738 Notes: The substrate structure are assumed.
| Organism (ID 9805): Arabidopsis thaliana
Full structure (ID 34125):
[bDManp(1-?)bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn&highlight=2~3~aLFuc) | Ohashi et al. 2017
DOI: 10.1016/j.bbrc.2016.12.087
|
Enzyme name: AtFUT12
UniProt ID: Q9FX97.1
CAZy family: GT10
Gene name: FUT12 / FucTB / AT1G49710
Gene GenBank ID: 841394 | Synthesized dimer: aLFucp(1-3)bDGlcpN1N
Donor (ID 34115): ?LFucp(1-P-P-5)xXnucG
Acceptor (ID 34121): bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn
Status: indirect evidence in vivo
Confirmation methods: mutation
ID: 1740 Notes: Suggested activity in planta. Both AtFUT11 and AtFUT12 are required for synthesis
of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN,
bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN
in
planta.
| Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: leaf, silique, stem
Full structure (ID 34122):
| Yoo et al. 2015
DOI: 10.1074/jbc.M115.653162
Strasser et al. 2004
DOI: 10.1016/S0014-5793(04)00150-4
Strasser et al. 2007
DOI: 10.1105/tpc.107.052985
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
1735 AtFUT11 Q9LJK1.1 GT10 FUT11 / FucTA / AT3G19280 821462 34118 aLFucp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn 0 aLFucp(1-3)bDGlcpN1N 2~3 ?LFucp(1-P-P-5)xXnucG Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn in vivo in vitro (crude extract), mutation Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN in planta. 0 0 9805 Arabidopsis thaliana Strasser et al. 2004 (doi: 10.1016/S0014-5793(04)00150-4); Wilson et al. 2001 (doi: 10.1016/S0304-4165(01)00151-9)
1736 AtFUT11 Q9LJK1.1 GT10 FUT11 / FucTA / AT3G19280 821462 34120 aLFucp(1-3)[aLFucp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn 0 aLFucp(1-3)bDGlcpN1N 2~3 ?LFucp(1-P-P-5)xXnucG Ac(1-2)bDGlcpN(1-2)aDManp(1-3)[Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[aLFucp(1-6),Ac(1-2)]bDGlcpN1N(1-4)x?Asn in vivo in vitro (purified protein), mutation Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN in planta. 0 0 9805 Arabidopsis thaliana Yoo et al. 2015 (doi: 10.1074/jbc.M115.653162); Strasser et al. 2004 (doi: 10.1016/S0014-5793(04)00150-4); Both et al. 2011 (doi: 10.1093/glycob/cwr056)
1737 AtFUT11 Q9LJK1.1 GT10 FUT11 / FucTA / AT3G19280 821462 34122 aLFucp(1-3)[bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn 0 aLFucp(1-3)bDGlcpN1N 2~3 ?LFucp(1-P-P-5)xXnucG bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn indirect mutation Suggested activity in planta. Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN in planta. 0 0 9805 Arabidopsis thaliana Strasser et al. 2004 (doi: 10.1016/S0014-5793(04)00150-4); Yoo et al. 2015 (doi: 10.1074/jbc.M115.653162)
1738 AtFUT11 Q9LJK1.1 GT10 FUT11 / FucTA / AT3G19280 821462 34125 aLFucp(1-3)[bDManp(1-?)bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn 0 aLFucp(1-3)bDGlcpN1N 2~3 ?LGalp(1-P-P-5)xXnucG bDManp(1-?)bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn in vitro in vitro (purified protein) The substrate structure are assumed. 0 0 9805 Arabidopsis thaliana Ohashi et al. 2017 (doi: 10.1016/j.bbrc.2016.12.087)
1740 AtFUT12 Q9FX97.1 GT10 FUT12 / FucTB / AT1G49710 841394 34122 aLFucp(1-3)[bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4),Ac(1-2)]bDGlcpN1N(1-4)x?Asn 0 aLFucp(1-3)bDGlcpN1N 2~3 ?LFucp(1-P-P-5)xXnucG bDXylp(1-2)[aDManp(1-6),Ac(1-2)bDGlcpN(1-2)aDManp(1-3)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN1N(1-4)x?Asn indirect mutation Suggested activity in planta. Both AtFUT11 and AtFUT12 are required for synthesis of bDXylp(1-2)[aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN, bDXylp(1-2)[Ac(1-2)bDGlcpN(1-2)aDManp(1-3),Ac(1-2)bDGlcpN(1-2)aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2),aLFucp(1-3)]bDGlcpN in planta. 0 0 9805 Arabidopsis thaliana leaf, silique, stem Yoo et al. 2015 (doi: 10.1074/jbc.M115.653162); Strasser et al. 2004 (doi: 10.1016/S0014-5793(04)00150-4); Strasser et al. 2007 (doi: 10.1105/tpc.107.052985)