| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: WaaR/WaaJ
UniProt ID: O68204* 
CAZy family: GT8
Gene name: waaR / waaJ / rfaJ
Gene GenBank ID: 948142* | Synthesized dimer: aDGlcp(1-2)aDGalp
aDGalp)
Acceptor (ID 34434): aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core
Status: evidence in vitro
Confirmation methods: overexpression in a non-native R3 background
ID: 2125 Notes: WaaR was able to extend the LPS acceptor from CWG350 (which bears an insertional
muration in waaJ) to generate a product that comigrated with the wild-type LPS,
despite the fact that the native acceptor residue for WaaR is a 1,3-linked Glc,
rather than the 1,3-linked Gal provided by CWG350 LPS.
| Organism (ID 16637): Escherichia coli R3 F653
Full structure (ID 34435):
aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core&highlight=3~2~aDGlc)
Molecule role (analog of): outer core | Leipold et al. 2007b
DOI: 10.1074/jbc.M704131200
|
Synthesized dimer: aDGlcp(1-2)aDGalp
Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU
Acceptor (ID 34434): aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core
Status: direct evidence in vivo
Confirmation methods: mutation (insertion), complementation, in vitro (purified protein), mutation (in recombinant protein)
ID: 2127 Notes: LPS isolated from E. coli CWG350, which bears an insertional mutation in waaJ, was
used as acceptor.
| Organism (ID 16637): Escherichia coli R3 F653
Full structure (ID 34438):
[Subst1(1-?)]aDGlcp(1-2)[Ac(1-2)aDGlcpN(1-3)]aDGalp(1-3)aDGlcp(1-3)Subst2 // Subst1 = O-antigen; Subst2 = inner core&highlight=3~2~aDGlc)
Molecule role: outer core | Leipold et al. 2007b
DOI: 10.1074/jbc.M704131200
Leipold et al. 2007a
DOI: 10.1074/jbc.M608164200
|
Enzyme name: WaaD
UniProt ID: Q8XDC3
CAZy family: GT4
| Synthesized dimer: aDGlcp(1-2)aDGalp
Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU
Status: direct evidence in vivo
Confirmation methods: in vitro (crude extract), mutation, complementation
ID: 2150 Notes: Endogenous substrate was used.
| Organism (ID 20): Escherichia coli O157:H7
Full structure (ID 34437):
[aDGlcp(1-2)aDGlcp(1-2)[aDGlcpN(1-3)]aDGalp(1-3)aDGlcp(1-3)[Ac(1-2)%25aDGlcpN(1-7)aXLDmanHepp(1-7)]aXLDmanHepp(1-3)[P-4)]aXLDmanHepp(1-5)]aXKdo(2-6)[P-4)]bDGlcpN(1-6)aDGlcpN(1-P&highlight=8~2~aDGlc)
Molecule role: outer core | Kaniuk et al. 2004
DOI: 10.1074/jbc.M401879200
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
2125 WaaR/WaaJ O68204 * GT8 waaR / waaJ / rfaJ 948142 * 34435 aDGlcp(1-2)aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core 0 aDGlcp(1-2)aDGalp 3~2 aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core in vitro overexpression in a non-native R3 background WaaR was able to extend the LPS acceptor from CWG350 (which bears an insertional muration in waaJ) to generate a product that comigrated with the wild-type LPS, despite the fact that the native acceptor residue for WaaR is a 1,3-linked Glc, rather than the 1,3-linked Gal provided by CWG350 LPS. outer core 1 0 16637 Escherichia coli R3 F653 Leipold et al. 2007b (doi: 10.1074/jbc.M704131200)
2127 WaaR/WaaJ O68204 * GT8 waaR / waaJ / rfaJ 948142 * 34438 aDGlcp(1-2)[Subst1(1-?)]aDGlcp(1-2)[Ac(1-2)aDGlcpN(1-3)]aDGalp(1-3)aDGlcp(1-3)Subst2 // Subst1 = O-antigen; Subst2 = inner core 0 aDGlcp(1-2)aDGalp 3~2 ?DGlcp(1-P-P-5)xXnucU aDGalp(1-3)aDGlcp(1-3)Subst // Subst = inner core in vivo mutation (insertion), complementation, in vitro (purified protein), mutation (in recombinant protein) LPS isolated from E. coli CWG350, which bears an insertional mutation in waaJ, was used as acceptor. outer core 0 0 16637 Escherichia coli R3 F653 Leipold et al. 2007b (doi: 10.1074/jbc.M704131200); Leipold et al. 2007a (doi: 10.1074/jbc.M608164200)
2150 WaaD Q8XDC3 GT4 34437 aXKdo(2-4)[aDGlcp(1-2)aDGlcp(1-2)[aDGlcpN(1-3)]aDGalp(1-3)aDGlcp(1-3)[Ac(1-2)%aDGlcpN(1-7)aXLDmanHepp(1-7)]aXLDmanHepp(1-3)[P-4)]aXLDmanHepp(1-5)]aXKdo(2-6)[P-4)]bDGlcpN(1-6)aDGlcpN(1-P 0 aDGlcp(1-2)aDGalp 8~2 ?DGlcp(1-P-P-5)xXnucU in vivo in vitro (crude extract), mutation, complementation Endogenous substrate was used. outer core 0 0 20 Escherichia coli O157:H7 Kaniuk et al. 2004 (doi: 10.1074/jbc.M401879200)