CSDB glycosyltransferase search

6 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene	Activity	Object	References
Enzyme name: alg1p UniProt ID: P16661 CAZy family: GT33 Gene name: ALG1 Gene GenBank ID: 852407	Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34827): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol Status: evidence in vitro Confirmation methods: in vitro (purified protein); expression in E.coli; mutation (in recombinant protein); in vitro (crude extract); mutation ID: 232 Notes: Recombinant Alg1 overexpressed in S. cerevisiae is responsible for adding first mannose residue. Yeast cells containing the alg1-1 mutation are temperature-sensitive for growth	Organism (ID 16684,16770,16876,16877): Saccharomyces cerevisiae PRY46, S288c, DBY 640, PRY56 Expressed in: E. coli Full structure (ID 34828): CSDB ID(s): 60991, 60992, 60993, 60994, 61004, 146987, 146988, 146989, 246988 Molecule role (precursor of): N-glycan	O’Reilly et al. 2006 DOI: 10.1021/bi060878o Couto et al. 1984 PMID: 6368538 Cueva et al. 1998 DOI: 10.1002/(SICI)1097-0061(19980615)14:8<773::AID-YEA284>3.0.CO%3B2-S Gao et al. 2004 DOI: 10.1093/glycob/cwh072
Enzyme name: alg1p UniProt ID: P16661* CAZy family: GT33 Gene name: ALG1 Gene GenBank ID: 852407*	Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34827): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol Status: direct evidence in vivo Confirmation methods: in vitro (membrane preparation); mutation (in recombinant protein), mutation ID: 233 Notes: Alg1-1 cells are unable to synthesize any mannose-containing oligosaccharide-lipids, indicating a block at the addition of the first mannose residue. The synthesis of asparagine-linked oligosaccharides involves the formation of the Glc3Man9GlcNAc2 oligosaccharide. The alg1 mutant accumulated lipid-linked GlcNAc2.	Organism (ID 9823,9862,16770,16878,16879,16880,16881,16882,16883,16884,16885,16886): Saccharomyces cerevisiae DBY640, DBY1033, S288c, DBY741, HMSF176, A364A, RS868, W303-1b, PRY55, PRY212, PRY95, RSY27 Full structure (ID 34829): CSDB ID(s): 60994 Molecule role (precursor of): N-glycan	Couto et al. 1984 PMID: 6368538 Huffaker et al. 1982 PMID: 7037780 Huffaker et al. 1983 DOI: 10.1073/pnas.80.24.7466 Roos et al. 1994 DOI: 10.1073/pnas.91.4.1485 Albright et al. 1990 PMID: 2182636 Wilson et al. 1995 DOI: 10.1042/bj3100909
Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34830): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt Status: evidence in vitro Confirmation methods: expression in E. coli; in vitro (in recombinant protein); in vitro (crude extract); in vitro (mutation) ID: 234 Notes: A chemically synthesized dolichol-linked oligosaccharide analogue, phytanyl-pyrophosphoryl-α-N, N′-diacetylchitobioside (PPGn2), was used as the acceptor for the β-1, 4 mannosyl transfer reaction	Organism (ID 9815): Saccharomyces cerevisiae X2180-1A Expressed in: E. coli Full structure (ID 34831): Molecule role (analog of): N-glycan	Wilson et al. 1995 DOI: 10.1042/bj3100909 Li et al. 2017b DOI: 10.1016/j.bbagen.2016.09.023 Revers et al. 1994 DOI: 10.1042/bj2990023 Revers et al. 1999 DOI: 10.1016/S0304-4165(99)00048-3 Watt et al. 1998 DOI: 10.1016/S0008-6215(97)00261-9 Watt et al. 1997 DOI: 10.1002/anie.199723541 Flitsch et al. 1992 DOI: 10.1039/p19920002087 Li et al. 2019 DOI: 10.1038/s41467-019-09752-3
Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34827): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol Status: evidence in vitro Confirmation methods: expression in E. coli; in vitro (in recombinant protein); in vitro (membrane preparation) ID: 235 Notes: The dolichyl- and phytanyl-linked substrates were easily mannosylated to form Man5GlcNAc2 at low concentrations of lipid-linked substrate	Organism (ID 9815): Saccharomyces cerevisiae X2180-1A Expressed in: E. coli Full structure (ID 34832): CSDB ID(s): 60991, 146989 Molecule role (analog of): N-glycan	Huffaker et al. 1983 DOI: 10.1073/pnas.80.24.7466 Wilson et al. 1995 DOI: 10.1042/bj3100909
Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34830): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt Status: evidence in vitro Confirmation methods: expression in E. coli; in vitro (in recombinant protein); in vitro (membrane preparation) ID: 236 Notes: The dolichyl- and phytanyl-linked substrates were easily mannosylated to form Man5GlcNAc2 at low concentrations of lipid-linked substrate	Organism (ID 9815): Saccharomyces cerevisiae X2180-1A Expressed in: E. coli Full structure (ID 34833): Molecule role (analog of): N-glycan	Wilson et al. 1995 DOI: 10.1042/bj3100909 Li et al. 2019 DOI: 10.1038/s41467-019-09752-3
Synthesized dimer: bDManp(1-4)bDGlcpN Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 35237): Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]bDGlcpN(1-P-P-1)Subst // Subst = dolichol Status: indirect evidence in vivo Confirmation methods: complementation ID: 500 Notes: Human Hmat-1 gene can complement the yeast alg1-1 mutation	Organism (ID 230): Homo sapiens Expressed in: S. cerevisiae Exact full structure is unknown Molecule role (precursor of): N-glycan	Takahashi et al. 2000 DOI: 10.1093/glycob/10.3.321

#GTR ID	Enzyme name	Enzyme group	Enzyme Genbank ID	Derived ID mark	Enzyme Uniprot ID	Derived ID mark	CAZY family	Gene name	Gene Genbank ID	Derived ID mark	Gene cluster	Full structure CSDB structure ID	Side product CSDB structure ID	Full structure	Full structure is fragment (0|1)	Synthesized bond	Synthesized bond location (from root ~ subst position)	Donor structure ID	Substrate structure	Confirmation status	Methods used	Notes	Molecule role	Molecule is a model of role (0|1)	Molecule is a precursor of role (0|1)	Main CSDB record ID	Additional CSDB record IDs	CSDB organism IDs	Organism names	Organs and tissues	References (;-separated)
232	alg1p				P16661		GT33	ALG1	852407			34828		bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	0	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	in vitro	in vitro (purified protein); expression in E.coli; mutation (in recombinant protein); in vitro (crude extract); mutation	Recombinant Alg1 overexpressed in S. cerevisiae is responsible for adding first mannose residue. Yeast cells containing the alg1-1 mutation are temperature-sensitive for growth	N-glycan	0	1		60991,60992,60993,60994,61004,146987,146988,146989,246988	16684,16770,16876,16877	Saccharomyces cerevisiae PRY46, S288c, DBY 640, PRY56	E. coli	O’Reilly et al. 2006 (doi: 10.1021/bi060878o); Couto et al. 1984 (doi:    pmid: 6368538); Cueva et al. 1998 (doi: 10.1002/(SICI)1097-0061(19980615)14:8<773::AID-YEA284>3.0.CO%3B2-S); Gao et al. 2004 (doi: 10.1093/glycob/cwh072)	
233	alg1p				P16661	*	GT33	ALG1	852407	*		34829		aDGlcp(1-2)aDGlcp(1-3)aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	0	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	in vivo	in vitro (membrane preparation); mutation (in recombinant protein), mutation	Alg1-1 cells are unable to synthesize any mannose-containing oligosaccharide-lipids, indicating a block at the addition of the first mannose residue. The synthesis of asparagine-linked oligosaccharides involves the formation of the Glc3Man9GlcNAc2 oligosaccharide. The alg1 mutant accumulated lipid-linked GlcNAc2.	N-glycan	0	1		60994	9823,9862,16770,16878,16879,16880,16881,16882,16883,16884,16885,16886	Saccharomyces cerevisiae DBY640, DBY1033, S288c, DBY741, HMSF176, A364A, RS868, W303-1b, PRY55, PRY212, PRY95, RSY27		Couto et al. 1984 (doi:    pmid: 6368538); Huffaker et al. 1982 (doi:    pmid: 7037780); Huffaker et al. 1983 (doi: 10.1073/pnas.80.24.7466); Roos et al. 1994 (doi: 10.1073/pnas.91.4.1485); Albright et al. 1990 (doi:    pmid: 2182636); Wilson et al. 1995 (doi: 10.1042/bj3100909)	
234	alg1p				P16661	*	GT33	ALG1	852407	*		34831		bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt	0	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt	in vitro	expression in E. coli; in vitro (in recombinant protein); in vitro (crude extract); in vitro (mutation)	A chemically synthesized dolichol-linked oligosaccharide analogue, phytanyl-pyrophosphoryl-α-N, N′-diacetylchitobioside (PPGn2), was used as the acceptor for the β-1, 4 mannosyl transfer reaction	N-glycan	1	0			9815	Saccharomyces cerevisiae X2180-1A	E. coli	Wilson et al. 1995 (doi: 10.1042/bj3100909); Li et al. 2017b (doi: 10.1016/j.bbagen.2016.09.023); Revers et al. 1994 (doi: 10.1042/bj2990023); Revers et al. 1999 (doi: 10.1016/S0304-4165(99)00048-3); Watt et al. 1998 (doi: 10.1016/S0008-6215(97)00261-9); Watt et al. 1997 (doi: 10.1002/anie.199723541); Flitsch et al. 1992 (doi: 10.1039/p19920002087); Li et al. 2019 (doi: 10.1038/s41467-019-09752-3)	
235	alg1p				P16661	*	GT33	ALG1	852407	*		34832		aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	0	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Subst // Subst = dolichol	in vitro	expression in E. coli; in vitro (in recombinant protein); in vitro (membrane preparation)	The dolichyl- and phytanyl-linked substrates were easily mannosylated to form Man5GlcNAc2 at low concentrations of lipid-linked substrate	N-glycan	1	0		60991,146989	9815	Saccharomyces cerevisiae X2180-1A	E. coli	Huffaker et al. 1983 (doi: 10.1073/pnas.80.24.7466); Wilson et al. 1995 (doi: 10.1042/bj3100909)	
236	alg1p				P16661	*	GT33	ALG1	852407	*		34833		aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt	0	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]aDGlcpN(1-P-P-1)Phyt	in vitro	expression in E. coli; in vitro (in recombinant protein); in vitro (membrane preparation)	The dolichyl- and phytanyl-linked substrates were easily mannosylated to form Man5GlcNAc2 at low concentrations of lipid-linked substrate	N-glycan	1	0			9815	Saccharomyces cerevisiae X2180-1A	E. coli	Wilson et al. 1995 (doi: 10.1042/bj3100909); Li et al. 2019 (doi: 10.1038/s41467-019-09752-3)	
500	alg1p				P16661	*	GT33	ALG1	852407	*		34834		bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol	1	bDManp(1-4)bDGlcpN	5~4	?DManp(1-P-P-5)xXnucG	Ac(1-2)bDGlcpN(1-4)[Ac(1-2)]bDGlcpN(1-P-P-1)Subst // Subst = dolichol	indirect	complementation	Human Hmat-1 gene can complement the yeast alg1-1 mutation	N-glycan	0	1			230	Homo sapiens 	S. cerevisiae	Takahashi et al. 2000 (doi: 10.1093/glycob/10.3.321)