CSDB glycosyltransferase search

4 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene	Activity	Object	References
Enzyme name: alg2p UniProt ID: P43636* CAZy family: GT4 Gene name: ALG2 Gene GenBank ID: 852815*	Synthesized dimer: aDManp(1-3)bDManp Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34834): bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol Status: direct evidence in vivo Confirmation methods: in vitro (membrane preparation); in vitro (mutation); mutation; expression in E.coli ID: 239 Notes: Alg2 is responsible for adding second and third mannose residue (presumably Man(1->3) is added first).	Organism (ID 16662,16684,16727,16887): Saccharomyces cerevisiae SS328, PRY46, W303-1A, DBY747 Full structure (ID 34835): CSDB ID(s): 60991, 60992, 60993, 122768, 146989, 246988 Molecule role (precursor of): N-glycan	O’Reilly et al. 2006 DOI: 10.1021/bi060878o Kampf et al. 2009 DOI: 10.1074/jbc.M806416200
Synthesized dimer: aDManp(1-3)bDManp Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34836): bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Phyt Status: evidence in vitro Confirmation methods: expression in E. coli; in vitro (membrane preparation) ID: 241 Notes: Reconstitution of the lipid-linked oligosaccharide pathway is presented.	Organism (ID 9835): Saccharomyces cerevisiae Expressed in: E. coli Full structure (ID 34837): Molecule role (analog of): N-glycan	Li et al. 2019 DOI: 10.1038/s41467-019-09752-3
Enzyme name: WbdA / MtfA UniProt ID: Q9LC67* CAZy family: GT4	Synthesized dimer: aDManp(1-3)bDManp Donor (ID 34509): ?DManp(1-P-P-5)xXnucG Acceptor (ID 34528): aDManp(1-2)aDManp(1-2)aDManp(1-3)aDManp Status: direct evidence in vivo Confirmation methods: in vitro (purified protein), mutation, complementationfied protein) ID: 2244 Notes: Repeating unit of the O8 antigen.	Organism (ID 16644): Escherichia coli O8 CWG636 Full structure (ID 27): CSDB ID(s): 182, 267, 1509, 1712, 5183, 5764, 10714, 10884, 12488, 20220, 25952, 26334, 27166, 27531, 28347, 28352, 31567, 101045, 108705, 114536, 116159, 126012, 131838 Molecule role: O-antigen	Greenfield et al. 2012a DOI: 10.1074/jbc.M112.401000 Greenfield et al. 2012b DOI: 10.1074/jbc.M112.412577
Enzyme name: WclB UniProt ID: Q4KZ35 CAZy family: GT4 Gene cluster ID: AY654590	Synthesized dimer: aDManp(1-3)bDManp Status: predicted in silico Confirmation methods: in silico ID: 2602	Organism (ID 8180): Escherichia coli O59 Full structure (ID 11575): CSDB ID(s): 1566, 30055, 30572 Molecule role: O-antigen	Fontana et al. 2014 DOI: 10.1093/glycob/cwu011

#GTR ID	Enzyme name	Enzyme group	Enzyme Genbank ID	Derived ID mark	Enzyme Uniprot ID	Derived ID mark	CAZY family	Gene name	Gene Genbank ID	Derived ID mark	Gene cluster	Full structure CSDB structure ID	Side product CSDB structure ID	Full structure	Full structure is fragment (0|1)	Synthesized bond	Synthesized bond location (from root ~ subst position)	Donor structure ID	Substrate structure	Confirmation status	Methods used	Notes	Molecule role	Molecule is a model of role (0|1)	Molecule is a precursor of role (0|1)	Main CSDB record ID	Additional CSDB record IDs	CSDB organism IDs	Organism names	Organs and tissues	References (;-separated)
239	alg2p				P43636	*	GT4	ALG2	852815	*		34835		aDManp(1-3)[aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol	0	aDManp(1-3)bDManp	6~3	?DManp(1-P-P-5)xXnucG	bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol	in vivo	in vitro (membrane preparation); in vitro (mutation); mutation; expression in E.coli	Alg2 is responsible for adding second and third mannose residue (presumably Man(1->3) is added first).	N-glycan	0	1		60991,60992,60993,122768,146989,246988	16662,16684,16727,16887	Saccharomyces cerevisiae SS328, PRY46, W303-1A, DBY747		O’Reilly et al. 2006 (doi: 10.1021/bi060878o); Kampf et al. 2009 (doi: 10.1074/jbc.M806416200)	
241	alg2p				P43636	*	GT4	ALG2	852815	*		34837		aDManp(1-3)[aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Phyt	0	aDManp(1-3)bDManp	6~3	?DManp(1-P-P-5)xXnucG	bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Phyt	in vitro	expression in E. coli; in vitro (membrane preparation)	Reconstitution of the lipid-linked oligosaccharide pathway is presented.	N-glycan	1	0			9835	Saccharomyces cerevisiae 	E. coli	Li et al. 2019 (doi: 10.1038/s41467-019-09752-3)	
2244	WbdA / MtfA				Q9LC67	*	GT4					27		-3)bDManp(1-2)aDManp(1-2)aDManp(1-	0	aDManp(1-3)bDManp	1~3	?DManp(1-P-P-5)xXnucG	aDManp(1-2)aDManp(1-2)aDManp(1-3)aDManp	in vivo	in vitro (purified protein), mutation, complementationfied protein)	Repeating unit of the O8 antigen.	O-antigen	0	0		182,267,1509,1712,5183,5764,10714,10884,12488,20220,25952,26334,27166,27531,28347,28352,31567,101045,108705,114536,116159,126012,131838	16644	Escherichia coli O8 CWG636		Greenfield et al. 2012a (doi: 10.1074/jbc.M112.401000); Greenfield et al. 2012b (doi: 10.1074/jbc.M112.412577)	
2602	WclB				Q4KZ35		GT4				AY654590	11575		-6)[aDGalpA(1-3)]aDManp(1-3)aDManp(1-3)bDManp(1-3)[Ac(1-2)]aDGlcpN(1-	0	aDManp(1-3)bDManp	3~3			in silico	in silico		O-antigen	0	0		1566,30055,30572	8180	Escherichia coli O59		Fontana et al. 2014 (doi: 10.1093/glycob/cwu011)