| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: alg8p
UniProt ID: P40351* 
CAZy family: GT57
Gene name: ALG8
Gene GenBank ID: 854233* | Synthesized dimer: aDGlcp(1-3)aDGlcp
aDGlcp)
Donor (ID 34871): bDGlcp(1-P-1)Subst // Subst = dolichol
Acceptor (ID 34875): aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol
Status: direct evidence in vivo
Confirmation methods: mutation; in vitro (membrane preparation); complimentation
ID: 266 Notes: Cells carrying the alg8-1 mutation accumulate Glc1Man9GlcNAc2-lipid both in vivo
and in vitro. Alg5, alg6 and alg8 mutations do not show any detectable growth defect,
but introducition of these mutations into a strain with wbp1-1 mutation leads
to a severe growth defect.
| Organism (ID 9823,16662,16972,16973,9862): Saccharomyces cerevisiae DBY640, SS328, PRY107, YG0100, DBY1033
Full structure (ID 34876):
aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol&highlight=10~3~aDGlc)
CSDB ID(s): 60994, 61004Molecule role (precursor of): N-glycan | Runge et al. 1986
PMID: 3536907
Stagljar et al. 1994
DOI: 10.1073/pnas.91.13.5977
|
Enzyme name: WaaO
UniProt ID: Q9R9D1*
CAZy family: GT8
Gene name: waaO / rfaI
Gene GenBank ID: 948143* | Synthesized dimer: aDGlcp(1-3)aDGlcp
Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU
Status: indirect evidence in vivo
Confirmation methods: mutation (insertion), complementation
ID: 2117 | Organism (ID 9179): Escherichia coli R1 F470
Full structure (ID 34431):
aDGalp(1-2)[Subst1(1-3)bDGlcp(1-3)]aDGlcp(1-3)aDGlcp(1-3)aXLDmanHepp(1-?)Subst2 // Subst1 = O-polysaccharide; Subst2 = lipid A&highlight=3~3~aDGlc)
Molecule role: core | Heinrich et al. 1998b
DOI: 10.1074/jbc.273.45.29497
|
Synthesized dimer: aDGlcp(1-3)aDGlcp
Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU
Status: predicted in silico
Confirmation methods: in silico
ID: 2118 | Organism (ID 16636): Escherichia coli R4 F2513
Full structure (ID 34432):
aDGalp(1-2)[bDGalp(1-4)]aDGlcp(1-3)aDGlcp(1-3)aXLDmanHepp(1-?)Subst // Subst = lipid A&highlight=3~3~aDGlc)
Molecule role: core |
Synthesized dimer: aDGlcp(1-3)aDGlcp
Status: indirect evidence in vivo
Confirmation methods: mutation (deletion), complementation
ID: 2122 | Organism (ID 23): Escherichia coli K12
Full structure (ID 34433):
bDGlcpN(1-7)aXLDmanHepp(1-6)aDGlcp(1-2)aDGlcp(1-3)[aDGalp(1-6)]aDGlcp(1-3)Subst // Subst = inner core&highlight=2~3~aDGlc)
Molecule role: outer core | Shibayama et al. 1998
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
266 alg8p P40351 * GT57 ALG8 854233 * 34876 aDGlcp(1-3)aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol 0 aDGlcp(1-3)aDGlcp 10~3 bDGlcp(1-P-1)Subst // Subst = dolichol aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol in vivo mutation; in vitro (membrane preparation); complimentation Cells carrying the alg8-1 mutation accumulate Glc1Man9GlcNAc2-lipid both in vivo and in vitro. Alg5, alg6 and alg8 mutations do not show any detectable growth defect, but introducition of these mutations into a strain with wbp1-1 mutation leads to a severe growth defect. N-glycan 0 1 60994,61004 9823,16662,16972,16973,9862 Saccharomyces cerevisiae DBY640, SS328, PRY107, YG0100, DBY1033 Runge et al. 1986 (doi: pmid: 3536907); Stagljar et al. 1994 (doi: 10.1073/pnas.91.13.5977)
2117 WaaO Q9R9D1 * GT8 waaO / rfaI 948143 * 34431 aDGalp(1-2)aDGalp(1-2)[Subst1(1-3)bDGlcp(1-3)]aDGlcp(1-3)aDGlcp(1-3)aXLDmanHepp(1-?)Subst2 // Subst1 = O-polysaccharide; Subst2 = lipid A 0 aDGlcp(1-3)aDGlcp 3~3 ?DGlcp(1-P-P-5)xXnucU indirect mutation (insertion), complementation core 0 0 9179 Escherichia coli R1 F470 Heinrich et al. 1998b (doi: 10.1074/jbc.273.45.29497)
2118 WaaO Q9R9D1 * GT8 waaO / rfaI 948143 * 34432 aDGalp(1-2)aDGalp(1-2)[bDGalp(1-4)]aDGlcp(1-3)aDGlcp(1-3)aXLDmanHepp(1-?)Subst // Subst = lipid A 0 aDGlcp(1-3)aDGlcp 3~3 ?DGlcp(1-P-P-5)xXnucU in silico in silico core 0 0 16636 Escherichia coli R4 F2513 Heinrich et al. 1998b (doi: 10.1074/jbc.273.45.29497)
2122 WaaO Q9R9D1 * GT8 waaO / rfaI 948143 * 34433 Ac(1-2)bDGlcpN(1-7)aXLDmanHepp(1-6)aDGlcp(1-2)aDGlcp(1-3)[aDGalp(1-6)]aDGlcp(1-3)Subst // Subst = inner core 0 aDGlcp(1-3)aDGlcp 2~3 indirect mutation (deletion), complementation outer core 0 0 23 Escherichia coli K12 Shibayama et al. 1998 (doi: url: http://jb.asm.org/content/180/20/5313.long)