CSDB glycosyltransferase search

5 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene	Activity	Object	References
Enzyme name: alg10p UniProt ID: P50076* CAZy family: GT59 Gene name: DIE2 Gene GenBank ID: 853142*	Synthesized dimer: aDGlcp(1-2)aDGlcp Donor (ID 34871): bDGlcp(1-P-1)Subst // Subst = dolichol Acceptor (ID 34877): aDGlcp(1-3)aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); overexpression ID: 267 Notes: Hypoglycosylation of secreted proteins in alg10 deletion strains demonstrates that the terminal a-1,2-linked glucose residue is a key element in substrate recognition by the oligosaccharyltransferase.	Organism (ID 16662,16980,16979,16978,16977,16976,16975,16974): Saccharomyces cerevisiae SS328, YG125, YG730, YG650, YG653, YG654, YG651, YG428 Full structure (ID 34878): CSDB ID(s): 60994 Molecule role: N-glycan	Burda et al. 1998 DOI: 10.1093/glycob/8.5.455
Enzyme name: ALG10 UniProt ID: Q8L638.1 CAZy family: GT59 Gene name: At5g02410 Gene GenBank ID: 831764	Synthesized dimer: aDGlcp(1-2)aDGlcp Donor (ID 34046): ?DGlcp(1-P-1)Subst // Subst = dolichol Acceptor (ID 34047): aDGlcp(1-3)aDGlcp(1-3)a?Manp(1-2)a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-6)]a?Manp(1-6)]b?Manp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN(1-P-P-1)Subst // Subst = dolichol Status: indirect evidence in vivo Confirmation methods: mutation, complementation ID: 1685 Notes: The function is supposed in accordance with homology with yeast.	Organism (ID 9805): Arabidopsis thaliana Organ/tissue: root, stem, leaf Full structure (ID 28362): CSDB ID(s): 61004	Aebi 2014 DOI: 10.1007/978-4-431-54240-7_1 Farid et al. 2011 DOI: 10.1111/j.1365-313X.2011.04688.x
Enzyme name: WaaR/WaaJ UniProt ID: O68204* CAZy family: GT8 Gene name: waaR / waaJ / rfaJ Gene GenBank ID: 948142*	Synthesized dimer: aDGlcp(1-2)aDGlcp Status: indirect evidence in vivo Confirmation methods: mutation (deletion), mutation, complementation ID: 2124	Organism (ID 23): Escherichia coli K12 Full structure (ID 34433): Molecule role: outer core	Shibayama et al. 1999 DOI: 10.1016/S0378-1097(99)00127-5
Enzyme name: WaaD UniProt ID: Q8XDC3 CAZy family: GT4	Synthesized dimer: aDGlcp(1-2)aDGlcp Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU Status: direct evidence in vivo Confirmation methods: in vitro (purified protein), mutation, complementation ID: 2149 Notes: Endogenous substrate was used.	Organism (ID 20): Escherichia coli O157:H7 Full structure (ID 34437): Molecule role: outer core	Kaniuk et al. 2004 DOI: 10.1074/jbc.M401879200
Enzyme name: DGS UniProt ID: Q8KQL6 CAZy family: GT4	Synthesized dimer: aDGlcp(1-2)aDGlcp Donor (ID 33557): ?DGlcp(1-P-P-5)xXnucU Acceptor (ID 35263): aDGlcp(1-1)[LIP(1-3),LIP(1-2)]xDGro Status: evidence in vitro Confirmation methods: in vitro (crude extract), mutation (in vitro) ID: 3019	Organism (ID 5529): Acholeplasma laidlawii Full structure (ID 35264):	Ge et al. 2011 DOI: 10.1074/jbc.M110.138495 Wikstroem et al. 2009 DOI: 10.1074/jbc.M804482200

#GTR ID	Enzyme name	Enzyme group	Enzyme Genbank ID	Derived ID mark	Enzyme Uniprot ID	Derived ID mark	CAZY family	Gene name	Gene Genbank ID	Derived ID mark	Gene cluster	Full structure CSDB structure ID	Side product CSDB structure ID	Full structure	Full structure is fragment (0|1)	Synthesized bond	Synthesized bond location (from root ~ subst position)	Donor structure ID	Substrate structure	Confirmation status	Methods used	Notes	Molecule role	Molecule is a model of role (0|1)	Molecule is a precursor of role (0|1)	Main CSDB record ID	Additional CSDB record IDs	CSDB organism IDs	Organism names	Organs and tissues	References (;-separated)
267	alg10p				P50076	*	GT59	DIE2	853142	*		34878		aDGlcp(1-2)aDGlcp(1-3)aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol	0	aDGlcp(1-2)aDGlcp	11~2	bDGlcp(1-P-1)Subst // Subst = dolichol	aDGlcp(1-3)aDGlcp(1-3)aDManp(1-2)aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-3)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]?DGlcpN(1-4)[Ac(1-2)]?DGlcpN(1-P-P-1)Subst // Subst = dolichol	in vivo	mutation; in vitro (membrane preparation); overexpression	Hypoglycosylation of secreted proteins in alg10 deletion strains demonstrates that the terminal a-1,2-linked glucose residue is a key element in substrate recognition by the oligosaccharyltransferase.	N-glycan	0	0		60994	16662,16980,16979,16978,16977,16976,16975,16974	Saccharomyces cerevisiae SS328, YG125, YG730, YG650, YG653, YG654, YG651, YG428		Burda et al. 1998 (doi: 10.1093/glycob/8.5.455)	
1685	ALG10				Q8L638.1		GT59	At5g02410	831764			28362		aDGlcp(1-2)aDGlcp(1-3)aDGlcp(1-3)a?Manp(1-2)a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-6)]a?Manp(1-6)]b?Manp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN(1-P-P-1)Subst // Subst = dolichol	0	aDGlcp(1-2)aDGlcp	11~2	?DGlcp(1-P-1)Subst // Subst = dolichol	aDGlcp(1-3)aDGlcp(1-3)a?Manp(1-2)a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-3)[a?Manp(1-2)a?Manp(1-6)]a?Manp(1-6)]b?Manp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]bDGlcpN(1-P-P-1)Subst // Subst = dolichol	indirect	mutation, complementation	The function is supposed in accordance with homology with yeast.		0	0		61004	9805	Arabidopsis thaliana 	root, stem, leaf	Aebi 2014 (doi: 10.1007/978-4-431-54240-7_1); Farid et al. 2011 (doi: 10.1111/j.1365-313X.2011.04688.x)	
2124	WaaR/WaaJ				O68204	*	GT8	waaR / waaJ / rfaJ	948142	*		34433		Ac(1-2)bDGlcpN(1-7)aXLDmanHepp(1-6)aDGlcp(1-2)aDGlcp(1-3)[aDGalp(1-6)]aDGlcp(1-3)Subst // Subst = inner core	0	aDGlcp(1-2)aDGlcp	3~2			indirect	mutation (deletion), mutation, complementation		outer core	0	0			23	Escherichia coli K12		Shibayama et al. 1999 (doi: 10.1016/S0378-1097(99)00127-5)	
2149	WaaD				Q8XDC3		GT4					34437		aXKdo(2-4)[aDGlcp(1-2)aDGlcp(1-2)[aDGlcpN(1-3)]aDGalp(1-3)aDGlcp(1-3)[Ac(1-2)%aDGlcpN(1-7)aXLDmanHepp(1-7)]aXLDmanHepp(1-3)[P-4)]aXLDmanHepp(1-5)]aXKdo(2-6)[P-4)]bDGlcpN(1-6)aDGlcpN(1-P	0	aDGlcp(1-2)aDGlcp	9~2	?DGlcp(1-P-P-5)xXnucU		in vivo	in vitro (purified protein), mutation, complementation	Endogenous substrate was used.	outer core	0	0			20	Escherichia coli O157:H7		Kaniuk et al. 2004 (doi: 10.1074/jbc.M401879200)	
3019	DGS				Q8KQL6		GT4					35264		aDGlcp(1-2)aDGlcp(1-1)[LIP(1-3),LIP(1-2)]xDGro	0	aDGlcp(1-2)aDGlcp	2~2	?DGlcp(1-P-P-5)xXnucU	aDGlcp(1-1)[LIP(1-3),LIP(1-2)]xDGro	in vitro	in vitro (crude extract), mutation (in vitro)			0	0			5529	Acholeplasma laidlawii 		Ge et al. 2011 (doi: 10.1074/jbc.M110.138495); Wikstroem et al. 2009 (doi: 10.1074/jbc.M804482200)