CSDB glycosyltransferase search

6 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene	Activity	Object	References
Enzyme name: gpi1p UniProt ID: P53306* Gene name: GPI1 Gene GenBank ID: 853130*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33759): Ac(1-2)aDGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); expression in E. coli; complementation ID: 268 Notes: Gpi1p participates in the first step in glycosylphosphatidylinositol anchor synthesis. Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's temperature sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins interact with one another in vivo. The synthesized product is subsequently deacetylated by Gpi12p.	Organism (ID 16802,16981,16982,16983,16984,16985,16986): Saccharomyces cerevisiae PP-1B, XM37-10C, PP-1D, L5686, L5487, 9933-13A, JF1015 Full structure (ID 34880): Molecule role (precursor of): GPI	Orlean et al. 2012 DOI: 10.1534/genetics.112.144485 Leidich et al. 1996 DOI: 10.1074/jbc.271.44.27829 Leidich et al. 1995 DOI: 10.1074/jbc.270.22.13029 Fabre et al. 2005 DOI: 10.1111/j.1742-4658.2005.04551.x
Enzyme name: gpi2p UniProt ID: P46961* Gene name: GPI2 Gene GenBank ID: 856029*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33759): Ac(1-2)aDGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); in vitro (complementation); overexpression; expression in C. albicans ID: 271 Notes: Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's temperature sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins interact with one another in vivo. Gpi2p is a component of the GlcNAc-PI synthase complex. GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the first step of GPI biosynthesis in S. cerevisiae. The synthesized product is subsequently deacetylated by Gpi12p.	Organism (ID 16802,16858,16981,16985,16986,16987): Saccharomyces cerevisiae PP-1B, YPH500, XM37-10C, 9933-13A, JF1015, YMW3 Organ/tissue: C. albicans Full structure (ID 34880): Molecule role (precursor of): GPI	Orlean et al. 2012 DOI: 10.1534/genetics.112.144485 Leidich et al. 1996 DOI: 10.1074/jbc.271.44.27829 Leidich et al. 1995 DOI: 10.1074/jbc.270.22.13029 Kostova et al. 2000 DOI: 10.1042/bj3500815 Yadav et al. 2014 DOI: 10.1007/s10719-014-9536-8
Enzyme name: gpi3p UniProt ID: P32363* CAZy family: GT4 Gene name: SPT14 Gene GenBank ID: 855928*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33759): Ac(1-2)aDGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); in vitro (inhibition); in vitro (complementation); expression in E. coli, overexpression ID: 274 Notes: Spt14 mutant cells are defective in GPI anchoring due to a defect in the synthesis of GIcNAc-PI, the first step of GPI synthesis. Gpi3p is catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Gpi3p is the UDP-GlcNAc-binding subunit of the yeast GlcNAc-PI synthetic complex. The synthesized product is subsequently deacetylated by Gpi12p.	Organism (ID 16802,16981,16985,16986,16987,16988,16989,16990): Saccharomyces cerevisiae PP-1B, XM37-10C, 9933-13A, JF1015, YMW3, RH329-3B, RH329-3C, RH401-7C Expressed in: E. coli Full structure (ID 34880): Molecule role (precursor of): GPI	Orlean et al. 2012 DOI: 10.1534/genetics.112.144485 Leidich et al. 1995 DOI: 10.1074/jbc.270.22.13029 Kostova et al. 2000 DOI: 10.1042/bj3500815 Schonbachler et al. 1995 DOI: 10.1002/j.1460-2075.1995.tb07152.x Kostova et al. 2003 DOI: 10.1046/j.1432-1033.2003.03844.x
Enzyme name: gpi15p UniProt ID: P53961* Gene name: GPI15 Gene GenBank ID: 855690*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33944): Ac(1-2)?DGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); in vitro (mutation) ID: 282 Notes: GPI15 encodes a protein subunit involved in GPI biosynthesis. The synthesized product is subsequently deacetylated by Gpi12p.	Organism (ID 16987): Saccharomyces cerevisiae YMW3 Full structure (ID 34880): Molecule role (precursor of): GPI	Orlean et al. 2012 DOI: 10.1534/genetics.112.144485 Yan et al. 2001 DOI: 10.1002/yea.783
Enzyme name: gpi19p UniProt ID: Q04082* Gene name: GPI19 Gene GenBank ID: 852047*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33944): Ac(1-2)?DGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: direct evidence in vivo Confirmation methods: mutation; in vitro (membrane preparation); in vitro (mutation) ID: 286 Notes: Gpi19 protein associates with the yeast GPI-GlcNAc transferase in vivo, as judged by coimmuneprecipitation with the Gpi2 subunit. The synthesized product is subsequently deacetylated by Gpi12p.	Organism (ID 17045,17046,17047,17048,17049,17050,17051,17052,17053,17054,17055,17056,17057,17058,17059,17060,17061): Saccharomyces cerevisiae DL2987, DL3050, DL3087, DL3089, DL3091, DL3093, DL3095, DL3097, DL3099, DL3102, DL3103, DL3104, DL3105, DL3106, DL3107, DL3149, DL3151 Full structure (ID 34880): Molecule role (precursor of): GPI	Orlean et al. 2012 DOI: 10.1534/genetics.112.144485 Newman et al. 2005 DOI: 10.1128/EC.4.11.1801–1807.2005
Enzyme name: gpi2p UniProt ID: P46961* Gene name: GPI2 Gene GenBank ID: 856029*	Synthesized dimer: aDGlcpN(1-6)myoIno Donor (ID 33944): Ac(1-2)?DGlcpN(1-P-P-5)xXnucU Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro Status: indirect evidence in vivo Confirmation methods: expression in C. albicans ID: 501 Notes: GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the first step of GPI biosynthesis in S. cerevisiae	Organism (ID 17319): Candida albicans YPH500 Full structure (ID 34880): Molecule role (precursor of): GPI	Yadav et al. 2014 DOI: 10.1007/s10719-014-9536-8

Enzyme name: gpi1p
UniProt ID: P53306*

Gene name: GPI1
Gene GenBank ID: 853130*

Synthesized dimer: aDGlcpN(1-6)myoIno

Donor (ID 33759): Ac(1-2)aDGlcpN(1-P-P-5)xXnucU

Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro

Status: direct evidence in vivo
Confirmation methods: mutation; in vitro (membrane preparation); expression in E. coli; complementation

ID: 268

Notes: Gpi1p participates in the first step in glycosylphosphatidylinositol anchor synthesis.
Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's
temperature sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins
interact with one another in vivo. The synthesized product is subsequently
deacetylated by Gpi12p.

Organism (ID 16802,16981,16982,16983,16984,16985,16986): Saccharomyces cerevisiae PP-1B, XM37-10C, PP-1D, L5686, L5487, 9933-13A, JF1015

Full structure (ID 34880):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Leidich et al. 1996
DOI: 10.1074/jbc.271.44.27829

Leidich et al. 1995
DOI: 10.1074/jbc.270.22.13029

Fabre et al. 2005
DOI: 10.1111/j.1742-4658.2005.04551.x

Enzyme name: gpi2p
UniProt ID: P46961*

Gene name: GPI2
Gene GenBank ID: 856029*

Synthesized dimer: aDGlcpN(1-6)myoIno

ID: 271

Notes: Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's temperature
sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins interact
with one another in vivo. Gpi2p is a component of the GlcNAc-PI synthase
complex. GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase
(GPI-GnT) complex that catalyzes the first step of GPI biosynthesis
in S. cerevisiae. The synthesized product is subsequently deacetylated
by Gpi12p.

Organism (ID 16802,16858,16981,16985,16986,16987): Saccharomyces cerevisiae PP-1B, YPH500, XM37-10C, 9933-13A, JF1015, YMW3
Organ/tissue: C. albicans

Full structure (ID 34880):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Leidich et al. 1996
DOI: 10.1074/jbc.271.44.27829

Leidich et al. 1995
DOI: 10.1074/jbc.270.22.13029

Kostova et al. 2000
DOI: 10.1042/bj3500815

Yadav et al. 2014
DOI: 10.1007/s10719-014-9536-8

Enzyme name: gpi3p
UniProt ID: P32363*

CAZy family: GT4

Gene name: SPT14
Gene GenBank ID: 855928*

Synthesized dimer: aDGlcpN(1-6)myoIno

ID: 274

Notes: Spt14 mutant cells are defective in GPI anchoring due to a defect in the synthesis
of GIcNAc-PI, the first step of GPI synthesis. Gpi3p is catalytic subunit in the
complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine
to phosphatidylinositol, the first step of GPI biosynthesis. Gpi3p is the
UDP-GlcNAc-binding subunit of the yeast GlcNAc-PI synthetic complex. The synthesized
product is subsequently deacetylated by Gpi12p.

Organism (ID 16802,16981,16985,16986,16987,16988,16989,16990): Saccharomyces cerevisiae PP-1B, XM37-10C, 9933-13A, JF1015, YMW3, RH329-3B, RH329-3C, RH401-7C
Expressed in: E. coli

Full structure (ID 34880):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Leidich et al. 1995
DOI: 10.1074/jbc.270.22.13029

Kostova et al. 2000
DOI: 10.1042/bj3500815

Schonbachler et al. 1995
DOI: 10.1002/j.1460-2075.1995.tb07152.x

Kostova et al. 2003
DOI: 10.1046/j.1432-1033.2003.03844.x

Enzyme name: gpi15p
UniProt ID: P53961*

Gene name: GPI15
Gene GenBank ID: 855690*

Synthesized dimer: aDGlcpN(1-6)myoIno

Donor (ID 33944): Ac(1-2)?DGlcpN(1-P-P-5)xXnucU

Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro

Status: direct evidence in vivo
Confirmation methods: mutation; in vitro (membrane preparation); in vitro (mutation)

ID: 282

Notes: GPI15 encodes a protein subunit involved in GPI biosynthesis. The synthesized product
is subsequently deacetylated by Gpi12p.

Organism (ID 16987): Saccharomyces cerevisiae YMW3

Full structure (ID 34880):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Yan et al. 2001
DOI: 10.1002/yea.783

Enzyme name: gpi19p
UniProt ID: Q04082*

Gene name: GPI19
Gene GenBank ID: 852047*

Synthesized dimer: aDGlcpN(1-6)myoIno

ID: 286

Notes: Gpi19 protein associates with the yeast GPI-GlcNAc transferase in vivo, as judged
by coimmuneprecipitation with the Gpi2 subunit. The synthesized product is subsequently
deacetylated by Gpi12p.

Organism (ID 17045,17046,17047,17048,17049,17050,17051,17052,17053,17054,17055,17056,17057,17058,17059,17060,17061): Saccharomyces cerevisiae DL2987, DL3050, DL3087, DL3089, DL3091, DL3093, DL3095, DL3097, DL3099, DL3102, DL3103, DL3104, DL3105, DL3106, DL3107, DL3149, DL3151

Full structure (ID 34880):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Newman et al. 2005
DOI: 10.1128/EC.4.11.1801–1807.2005

Enzyme name: gpi2p
UniProt ID: P46961*

Gene name: GPI2
Gene GenBank ID: 856029*

Synthesized dimer: aDGlcpN(1-6)myoIno

Donor (ID 33944): Ac(1-2)?DGlcpN(1-P-P-5)xXnucU

Acceptor (ID 34879): LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro

Status: indirect evidence in vivo
Confirmation methods: expression in C. albicans

ID: 501

Notes: GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase
(GPI-GnT) complex that catalyzes the first step of GPI biosynthesis
in S. cerevisiae

Organism (ID 17319): Candida albicans YPH500

Full structure (ID 34880):

Molecule role (precursor of): GPI

Yadav et al. 2014
DOI: 10.1007/s10719-014-9536-8

#GTR ID	Enzyme name	Enzyme group	Enzyme Genbank ID	Derived ID mark	Enzyme Uniprot ID	Derived ID mark	CAZY family	Gene name	Gene Genbank ID	Derived ID mark	Gene cluster	Full structure CSDB structure ID	Side product CSDB structure ID	Full structure	Full structure is fragment (0|1)	Synthesized bond	Synthesized bond location (from root ~ subst position)	Donor structure ID	Substrate structure	Confirmation status	Methods used	Notes	Molecule role	Molecule is a model of role (0|1)	Molecule is a precursor of role (0|1)	Main CSDB record ID	Additional CSDB record IDs	CSDB organism IDs	Organism names	Organs and tissues	References (;-separated)
268	gpi1p				P53306	*		GPI1	853130	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)aDGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	in vivo	mutation; in vitro (membrane preparation); expression in E. coli; complementation	Gpi1p participates in the first step in glycosylphosphatidylinositol anchor synthesis. Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's temperature sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins interact with one another in vivo. The synthesized product is subsequently deacetylated by Gpi12p.	GPI	0	1			16802,16981,16982,16983,16984,16985,16986	Saccharomyces cerevisiae PP-1B, XM37-10C, PP-1D, L5686, L5487, 9933-13A, JF1015		Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Leidich et al. 1996 (doi: 10.1074/jbc.271.44.27829); Leidich et al. 1995 (doi: 10.1074/jbc.270.22.13029); Fabre et al. 2005 (doi: 10.1111/j.1742-4658.2005.04551.x)	
271	gpi2p				P46961	*		GPI2	856029	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)aDGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	in vivo	mutation; in vitro (membrane preparation); in vitro (complementation); overexpression; expression in C. albicans	Overexpression of the GPI2 gene causes partial suppression of the gpi1 mutant's temperature sensitivity, a result that suggests that the Gpi1 and Gpi2 proteins interact with one another in vivo. Gpi2p is a component of the GlcNAc-PI synthase complex. GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the first step of GPI biosynthesis in S. cerevisiae. The synthesized product is subsequently deacetylated by Gpi12p.	GPI	0	1			16802,16858,16981,16985,16986,16987	Saccharomyces cerevisiae PP-1B, YPH500, XM37-10C, 9933-13A, JF1015, YMW3	 C. albicans 	Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Leidich et al. 1996 (doi: 10.1074/jbc.271.44.27829); Leidich et al. 1995 (doi: 10.1074/jbc.270.22.13029); Kostova et al. 2000 (doi: 10.1042/bj3500815); Yadav et al. 2014 (doi: 10.1007/s10719-014-9536-8)	
274	gpi3p				P32363	*	GT4	SPT14	855928	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)aDGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	in vivo	mutation; in vitro (membrane preparation); in vitro (inhibition); in vitro (complementation); expression in E. coli, overexpression	Spt14 mutant cells are defective in GPI anchoring due to a defect in the synthesis of GIcNAc-PI, the first step of GPI synthesis. Gpi3p is catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Gpi3p is the UDP-GlcNAc-binding subunit of the yeast GlcNAc-PI synthetic complex. The synthesized product is subsequently deacetylated by Gpi12p.	GPI	0	1			16802,16981,16985,16986,16987,16988,16989,16990	Saccharomyces cerevisiae PP-1B, XM37-10C, 9933-13A, JF1015, YMW3, RH329-3B, RH329-3C, RH401-7C	E. coli	Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Leidich et al. 1995 (doi: 10.1074/jbc.270.22.13029); Kostova et al. 2000 (doi: 10.1042/bj3500815); Schonbachler et al. 1995 (doi: 10.1002/j.1460-2075.1995.tb07152.x); Kostova et al. 2003 (doi: 10.1046/j.1432-1033.2003.03844.x)	
282	gpi15p				P53961	*		GPI15	855690	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)?DGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	in vivo	mutation; in vitro (membrane preparation); in vitro (mutation)	GPI15 encodes a protein subunit involved in GPI biosynthesis. The synthesized product is subsequently deacetylated by Gpi12p.	GPI	0	1			16987	Saccharomyces cerevisiae YMW3		Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Yan et al. 2001 (doi: 10.1002/yea.783)	
286	gpi19p				Q04082	*		GPI19	852047	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)?DGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	in vivo	mutation; in vitro (membrane preparation); in vitro (mutation)	Gpi19 protein associates with the yeast GPI-GlcNAc transferase in vivo, as judged by coimmuneprecipitation with the Gpi2 subunit. The synthesized product is subsequently deacetylated by Gpi12p.	GPI	0	1			17045,17046,17047,17048,17049,17050,17051,17052,17053,17054,17055,17056,17057,17058,17059,17060,17061	Saccharomyces cerevisiae DL2987, DL3050, DL3087, DL3089, DL3091, DL3093, DL3095, DL3097, DL3099, DL3102, DL3103, DL3104, DL3105, DL3106, DL3107, DL3149, DL3151		Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Newman et al. 2005 (doi: 10.1128/EC.4.11.1801–1807.2005)	
501	gpi2p				P46961	*		GPI2	856029	*		34880		LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro	0	aDGlcpN(1-6)xXmyoIno?	3~6	Ac(1-2)?DGlcpN(1-P-P-5)xXnucU	LIP(1-2)[LIP(1-3),xXmyoIno(1-P-1)]xDGro	indirect	expression in C. albicans	GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the first step of GPI biosynthesis in S. cerevisiae	GPI	0	1			17319	Candida albicans YPH500		Yadav et al. 2014 (doi: 10.1007/s10719-014-9536-8)