| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: gpi7p
UniProt ID: P40367* 
Gene name: GPI7/LAS21
Gene GenBank ID: 853384* | Synthesized dimer: P(?-6)aDManp
aDManp)
Donor (ID 34881): LIP(1-1)[LIP(1-3),xXEtN(1-P-2)]x?Gro
Acceptor (ID 34882): LIP(1-2)[LIP(1-3),aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-2)aDManp(1-6)[xXEtN(1-P-2)]aDManp(1-4)aDGlcpN(1-6)[LIP(1-?)]xXmyoIno(1-P-1)]xDGro
Status: direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; complementation; in vitro (mutation)
ID: 277 Notes: Gpi7p itself is the transferase adding a side chain to the a1,6-linked mannose of
the GPI core structure
| Organism (ID 9815,16879,16903,16991,16992,16993,16994,16995,16996,16997,16998,16999,17000,17001,17002): Saccharomyces cerevisiae X2180-1A, HMSF176, W303-1B, FBY11, FBY15, FBY122, FBY182, FBY49, C4, HMSF331, LB2134-3B, YNS3-7A, FBY118, FBY40, FBY43
Expressed in: E. coli
Full structure (ID 34883):
[LIP(1-3),aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-6)[xXEtN(1-P-2)]aDManp(1-4)aDGlcpN(1-6)[LIP(1-?)]xXmyoIno(1-P-1)]xDGro&highlight=6~6~xXP)
Molecule role (precursor of): GPI | Orlean et al. 2012
DOI: 10.1534/genetics.112.144485
Benachour et al. 1999
DOI: 10.1074/jbc.274.21.15251
Fujita et al. 2004
DOI: 10.1074/jbc.M405232200
Imhof et al. 2000
DOI: 10.1093/glycob/10.12.1271
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
277 gpi7p P40367 * GPI7/LAS21 853384 * 34883 LIP(1-2)[LIP(1-3),aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-6)[xXEtN(1-P-2)]aDManp(1-4)aDGlcpN(1-6)[LIP(1-?)]xXmyoIno(1-P-1)]xDGro 0 xXP?(?-6)aDManp 6~6 LIP(1-1)[LIP(1-3),xXEtN(1-P-2)]x?Gro LIP(1-2)[LIP(1-3),aDManp(1-2)[xXEtN(1-P-6)]aDManp(1-2)aDManp(1-6)[xXEtN(1-P-2)]aDManp(1-4)aDGlcpN(1-6)[LIP(1-?)]xXmyoIno(1-P-1)]xDGro in vivo mutation; expression in E. coli; complementation; in vitro (mutation) Gpi7p itself is the transferase adding a side chain to the a1,6-linked mannose of the GPI core structure GPI 0 1 9815,16879,16903,16991,16992,16993,16994,16995,16996,16997,16998,16999,17000,17001,17002 Saccharomyces cerevisiae X2180-1A, HMSF176, W303-1B, FBY11, FBY15, FBY122, FBY182, FBY49, C4, HMSF331, LB2134-3B, YNS3-7A, FBY118, FBY40, FBY43 E. coli Orlean et al. 2012 (doi: 10.1534/genetics.112.144485); Benachour et al. 1999 (doi: 10.1074/jbc.274.21.15251); Fujita et al. 2004 (doi: 10.1074/jbc.M405232200); Imhof et al. 2000 (doi: 10.1093/glycob/10.12.1271)