CSDB glycosyltransferase search

5 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene Activity Object References
Enzyme name: gpi14p
UniProt ID: P47088*
CAZy family: GT50

Gene name: GPI14
Gene GenBank ID: 853470*
Synthesized dimer: aDManp(1-4)aDGlcpN


Donor (ID 34645): ?DManp(1-P-1)Subst // Subst = dolichol

Acceptor (ID 34886): LIP(1-2)[LIP(1-3),aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro

Status: indirect evidence in vivo
Confirmation methods: mutation
ID: 280
Notes: Partial inactivation of Gpi14p causes defects in the cell wall structure. Gpi14p
is involved in the addition of the first mannose residue to the GPI anchor precursor.
Organism (ID 17016,17027,17028,17029,17030,17031,17032,17033,17034,17035,17036,17037,17038,17039,17040,17041): Saccharomyces cerevisiae FY1679, H1480, E0/YUG37, E37, H3204, H3205, H3211, H3212, H3213, H3238, H3263, H3293, H3296, H3302, BJ10242, BJ5410

Full structure (ID 34887):

Molecule role (precursor of): GPI

Orlean et al. 2012
DOI: 10.1534/genetics.112.144485

Imhof et al. 2000
DOI: 10.1093/glycob/10.12.1271

Davydenko et al. 2005
DOI: 10.1002/yea.1286

Subramanian et al. 2006
DOI: 10.1073 pnas.0505570103

Ashida et al. 2005
DOI: 10.1091/mbc.E04–09–0802

Naik at al. 1998
PMID: 9649520
Synthesized dimer: aDManp(1-4)aDGlcpN


Donor (ID 34645): ?DManp(1-P-1)Subst // Subst = dolichol

Acceptor (ID 34888): lXOco(1-2)[lXOco(1-3),aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro

Status: evidence in vitro
Confirmation methods: in vitro (membrane preparation)
ID: 281
Notes: The PIG-M homologues from humans, Plasmodium falciparum (PfPIG-M), and Saccharomyces
cerevisiae (GPI14) were cloned to test whether they could complement GPI-MT-I-deficient
mammalian cells
Organism (ID 9835): Saccharomyces cerevisiae

Full structure (ID 34889):

Molecule role (precursor of): GPI

Kim et al. 2007
PMID: 17978585
Synthesized dimer: aDManp(1-4)aDGlcpN


Donor (ID 34645): ?DManp(1-P-1)Subst // Subst = dolichol

Acceptor (ID 35242): LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro

Status: indirect evidence in vivo
Confirmation methods: mutation (in recombinant protein); expression in S. cerevisiae
ID: 507
Notes: Association of PIG-X and PIG-M for GPI-MT-I activity is not interchangeable between
mammals and the other lower eukaryotes
Organism (ID 17324): Homo sapiens W303
Expressed in: S. cerevisiae

Full structure (ID 34887):

Molecule role (precursor of): GPI

Kim et al. 2007b
DOI: 10.1093/jb/mvm113
Synthesized dimer: aDManp(1-4)aDGlcpN


Donor (ID 34645): ?DManp(1-P-1)Subst // Subst = dolichol

Acceptor (ID 35242): LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro

Status: indirect evidence in vivo
Confirmation methods: mutation (in recombinant protein); expression in S. cerevisiae
ID: 508
Notes: Association of PIG-X and PIG-M for GPI-MT-I activity is not interchangeable between
mammals and the other lower eukaryotes
Organism (ID 17325): Trypanosoma brucei W303
Expressed in: S. cerevisiae

Full structure (ID 34887):

Molecule role (precursor of): GPI

Synthesized dimer: aDManp(1-4)aDGlcpN


Donor (ID 34645): ?DManp(1-P-1)Subst // Subst = dolichol

Acceptor (ID 35242): LIP(1-2)[LIP(1-3),Ac(1-2)aDGlcpN(1-6)xXmyoIno(1-P-1)]xDGro

Status: indirect evidence in vivo
Confirmation methods: mutation (in recombinant protein); expression in S. cerevisiae
ID: 509
Notes: Association of PIG-X and PIG-M for GPI-MT-I activity is not interchangeable between
mammals and the other lower eukaryotes
Organism (ID 17326): Plasmodium falciparum W303
Expressed in: S. cerevisiae

Full structure (ID 34887):

Molecule role (precursor of): GPI

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