| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: ugt51p
UniProt ID: Q06321* 
CAZy family: GT1
Gene name: ATG26/UGT51
Gene GenBank ID: 850886* | Synthesized dimer: bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C)
Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU
Acceptor (ID 33971): Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 300 Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols as sugar acceptors
| Organism (ID 17220): Saccharomyces cerevisiae UTL-7A
Expressed in: E. coli
Full structure (ID 21921):
Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C&highlight=1~3~bDGlc)
CSDB ID(s): 49612, 60933, 62647, 62747, 63102, 64012, 64092, 65045, 65250, 65773, 66899, 67265, 67303Molecule role: membrane glycolipid | Warnecke et al. 1999
DOI: 10.1074/jbc.274.19.13048
|
Synthesized dimer: bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU
Acceptor (ID 33971): Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 513 Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
| Organism (ID 12218): Avena sativa
Expressed in: E. coli
Full structure (ID 21921):
CSDB ID(s): 49612, 62647, 62747, 63102, 64012, 64092, 65045, 65250, 65773, 66899, 67265, 67303, 299860Molecule role: membrane glycolipid |
Synthesized dimer: bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU
Acceptor (ID 33971): Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 518 Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
| Organism (ID 9097): Dictyostelium discoideum
Expressed in: E. coli
Full structure (ID 21921):
CSDB ID(s): 49612, 62647, 62747, 63102, 64012, 64092, 65045, 65250, 65773, 66899, 67265, 67303, 299860Molecule role: membrane glycolipid |
Synthesized dimer: bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU
Acceptor (ID 33971): Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C
Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 523 Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
| Organism (ID 10327): Pichia pastoris
Expressed in: E. coli
Full structure (ID 21921):
CSDB ID(s): 49612, 62647, 62747, 63102, 64012, 64092, 65045, 65250, 65773, 66899, 67265, 67303, 299860Molecule role: membrane glycolipid |
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
300 ugt51p Q06321 * GT1 ATG26/UGT51 850886 * 21921 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 0 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 1~3 aDGlcp(1-P-P-5)xXnucU Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C semi-direct mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation) UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different sterols as sugar acceptors membrane glycolipid 0 0 49612,60933,62647,62747,63102,64012,64092,65045,65250,65773,66899,67265,67303 17220 Saccharomyces cerevisiae UTL-7A E. coli Warnecke et al. 1999 (doi: 10.1074/jbc.274.19.13048)
513 ugt51p Q06321 * GT1 ATG26/UGT51 850886 * 21921 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 0 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 1~3 aDGlcp(1-P-P-5)xXnucU Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C semi-direct mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation) UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors membrane glycolipid 0 0 49612,62647,62747,63102,64012,64092,65045,65250,65773,66899,67265,67303,299860 12218 Avena sativa E. coli Warnecke et al. 1999 (doi: 10.1074/jbc.274.19.13048)
518 ugt51p Q06321 * GT1 ATG26/UGT51 850886 * 21921 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 0 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 1~3 aDGlcp(1-P-P-5)xXnucU Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C semi-direct mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation) UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors membrane glycolipid 0 0 49612,62647,62747,63102,64012,64092,65045,65250,65773,66899,67265,67303,299860 9097 Dictyostelium discoideum E. coli Warnecke et al. 1999 (doi: 10.1074/jbc.274.19.13048)
523 ugt51p Q06321 * GT1 ATG26/UGT51 850886 * 21921 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 0 bDGlcp(1-3)Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C 1~3 aDGlcp(1-P-P-5)xXnucU Subst // Subst = stigmasterol = SMILES CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC{3}[C@@H](C4)O)C)C)C(C)C semi-direct mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation) UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors membrane glycolipid 0 0 49612,62647,62747,63102,64012,64092,65045,65250,65773,66899,67265,67303,299860 10327 Pichia pastoris E. coli Warnecke et al. 1999 (doi: 10.1074/jbc.274.19.13048)