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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)

Publication DOI: 10.1016/j.carbpol.2018.08.104
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: Agata.Zykwinska

ifremer.fr; Sylvia.Colliec.Jouault

ifremer.fr
Institutions: Ifremer, Laboratoire Ecosystemes Microbiens et Molecules Marines pour les Biotechnologies, 44311 Nantes, France, INRA, UR1268 Biopolymeres Interactions Assemblages, F-44300 Nantes, France, Institut des Materiaux Jean Rouxel (IMN), Universite de Nantes-CNRS, 44322 Nantes, France

Sulfated polysaccharides, such as glycosaminoglycans (GAG) regulate various biological activities through their interactions with growth factors. Investigating these interactions becomes the key to understand the structure-function relationship of GAG. Highly sulfated derivatives prepared from the marine GY785 exopolysaccharide (EPS) produced by the deep-sea hydrothermal vent bacterium Alteromonas infernus have previously shown to stimulate the chondrogenic differentiation of mesenchymal stem cells in the presence of Transforming Growth Factor-β1 (TGF-β1). Here, the interactions between the GAG-mimetic GY785 EPS derivatives and TGF-β1 were investigated by Atomic Force Microscopy (AFM). The affinity between slightly sulfated or highly sulfated derivatives and TGF-β1 was explored by AFM imaging and single-molecule force spectroscopy experiments. The number of measured interactions and the interaction strength were both higher for highly sulfated derivative compared to the slightly sulfated one. These results clearly emphasize the involvement of sulfate groups in the protein binding and open new ways to tune cellular processes by designing macromolecules with adjustable sulfate charge density.

exopolysaccharide, glycosaminoglycans, AFM imaging, nanoassemblies, single-molecule force spectroscopy, sulfated polysaccharides

Structure type: polymer chemical repeating unit
Location inside paper: p.59, fig.1
Trivial name: infernan
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_423153,IEDB_983931,SB_192,SB_7

Methods: HPAEC, composition analysis, atomic force microscopy, zeta potential measurement, HPSEC-MALS, single-molecule force spectroscopy

NCBI Taxonomy refs (TaxIDs): 179275
Reference(s) to other database(s): GTC:G33666QQ, GlycomeDB:27442
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@@H](O[C@H]2[C@@H](C(=O)O)O[C@H](O[C@H]3[C@@H](CO)O[C@H]([*])[C@H](O)[C@H]3O)[C@H](OS(=O)(=O)O)[C@H]2O[C@@H]2O[C@H](C(=O)O)[C@@H](O[C@@H]3O[C@H](C(=O)O)[C@@H](O[C@H]4O[C@H](CO[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize