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Nakajima T, Yoshida M, Hiura N, Matsuda K
Structure of the cell wall proteogalactomannan from Neurospora crassa. I. Purification of the proteoheteroglycan and characterization of alkali- labile oligosaccharides
Journal of Biochemistry 96 (1984)
1005-1011
Neurospora crassa IFO-6068
(Ancestor NCBI TaxID 5141,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 6240490Publication DOI: 10.1093/oxfordjournals.jbchem.a134916Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Tohoku University, Tsutsumidori-Amamiyamachi, Sendai, Miyagi, Japan
Proteoheteroglycan (PHG) was prepared from Neurospora crassa cells by extraction with hot water followed by cetyltrimethylammoniumbromide fractionation. The polymer was purified by DEAE-cellulose chromatography followed by gel filtrations. The PHG was fractionated into five subfractions containing carbohydrate (65-88%), protein (19-36%), and a trace amount of phosphate (0.3-1.9%). The sugar compositions of the fractions were similar to each other (D-mannose, 47-60%, D-galactose, 35-50%, D-glucose, 2-5%) while the fractions showed significant heterogeneity in molecular size. Mild alkali treatment of the PHG in the presence of sodium borohydride yielded three kinds of reduced oligosaccharides. Structural studies using a methylation-GC-MS method, and proton and carbon NMR indicated that the tetrasaccharide fragment is β-D-Galf(1-5)-β-D-Galf(1-2)-α-D-Manp(1-2)mannitol, the trisaccharide is β-D-Galf(1-2)-α-D-Manp(1-2)mannitol, and the disaccharide is α-D-Manp(1-2)mannitol.
Structure type: oligomer
Location inside paper: Fig. 6
Contained glycoepitopes: IEDB_114705,IEDB_130701,IEDB_136095,IEDB_137472,IEDB_144983,IEDB_152206,IEDB_190606,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, GLC, paper chromatography, methylation (Hakomori method), precipitation, hot water extraction, mild alkali degradation
Comments, role: Parent molecule: proteogalactomannan;
Related record ID(s): 132224
NCBI Taxonomy refs (TaxIDs): 5141Reference(s) to other database(s): GTC:G39277TV, CCSD:
11373, CBank-STR:5779
Show glycosyltransferases
NMR conditions: in D2O at 343(C) K
[as TSV]
13C NMR data: present in publication
|
There is only one chemically distinct structure:
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Nakajima T, Yoshida M, Hiura N, Matsuda K
Structure of the cell wall proteogalactomannan from Neurospora crassa. I. Purification of the proteoheteroglycan and characterization of alkali- labile oligosaccharides
Journal of Biochemistry 96 (1984)
1005-1011
Neurospora crassa IFO-6068
(Ancestor NCBI TaxID 5141,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 6240490Publication DOI: 10.1093/oxfordjournals.jbchem.a134916Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Tohoku University, Tsutsumidori-Amamiyamachi, Sendai, Miyagi, Japan
Proteoheteroglycan (PHG) was prepared from Neurospora crassa cells by extraction with hot water followed by cetyltrimethylammoniumbromide fractionation. The polymer was purified by DEAE-cellulose chromatography followed by gel filtrations. The PHG was fractionated into five subfractions containing carbohydrate (65-88%), protein (19-36%), and a trace amount of phosphate (0.3-1.9%). The sugar compositions of the fractions were similar to each other (D-mannose, 47-60%, D-galactose, 35-50%, D-glucose, 2-5%) while the fractions showed significant heterogeneity in molecular size. Mild alkali treatment of the PHG in the presence of sodium borohydride yielded three kinds of reduced oligosaccharides. Structural studies using a methylation-GC-MS method, and proton and carbon NMR indicated that the tetrasaccharide fragment is β-D-Galf(1-5)-β-D-Galf(1-2)-α-D-Manp(1-2)mannitol, the trisaccharide is β-D-Galf(1-2)-α-D-Manp(1-2)mannitol, and the disaccharide is α-D-Manp(1-2)mannitol.
Structure type: oligomer
Location inside paper: Fig. 6
Contained glycoepitopes: IEDB_114705,IEDB_130701,IEDB_136095,IEDB_137472,IEDB_144983,IEDB_152206,IEDB_190606,IEDB_885812,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, GLC, paper chromatography, methylation (Hakomori method), precipitation, hot water extraction, mild alkali degradation
Comments, role: Parent molecule: proteogalactomannan;
Related record ID(s): 132223
NCBI Taxonomy refs (TaxIDs): 5141Reference(s) to other database(s): GTC:G17470DT, CCSD:
515, CBank-STR:10981
Show glycosyltransferases
NMR conditions: in D2O at 343(C) K
[as TSV]
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
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