The structure was elucidated in this paper NCBI PubMed ID:6368551 Journal NLM ID:2985121R Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology Institutions: University of Califonia, Berkeley, California, USA
The neutral oligosaccharides from Saccharomyces cerevisiae mnn1 mnn9, mnn2 mnn9, and mnn9 mutant mannoproteins, and from mnn1 and wild type carboxypeptidase Y, have been characterized. The major oligosaccharide from the mnn1 mnn9 mutant, Man10GlcNAc, has the structure (formula; see text) whereas the largest oligosaccharide from the mnn9 mutant, Man13GlcNAc, has the structure (formula; see text) the differences being due to the mnn1 mutation. The smaller mnn9 homologs had lesser amounts of terminal α 1----3-linked mannose and may be precursors of the mature oligosaccharide. The mnn2 mutation had no effect on the mnn9 oligosaccharide structures. Carboxypeptidase Y and mnn9 oligosaccharides were identical, which suggests that the mnn9 mutation eliminates the differences in carbohydrate structure that distinguish intra- from extracellular mannoproteins. One mnn1 mnn9 oligosaccharide, Man11GlcNAc, retained the terminal α 1----2-linked mannose of the lipid-linked core precursor, which suggests that processing to give the larger oligosaccharides can occur without removal of this unit. A smaller mnn1 mnn9 oligosaccharide, Man9GlcNAc, was a mixture of isomers that must, in part, have arisen by action of an α 1----2-mannosidase.
Methods: gel filtration, 1H NMR, FAB-MS, endoglucosaminidase H digestion, thin layer chromatography Biosynthesis and genetic data: The mnn9 mutation possibly eliminates the differences in carbohydrate structure that distinguish intra- from extracellular mannoproteins, whereas the mnnl mutation prevents addition of al->3-linked mannose to mannose in both a1->2 and a1->3 linkages. Comments, role: Parent molecule: mannoprotein; the strain used bears mutations in mnn1 and mnn9
Related record ID(s): 134185, 134682 NCBI Taxonomy refs (TaxIDs):4932 Reference(s) to other database(s): GTC:G65012BV, CCSD:16253, CBank-STR:19618 Show glycosyltransferases
There are 2 chemically distinct structures. Please, select:
The structure was elucidated in this paper NCBI PubMed ID:6368551 Journal NLM ID:2985121R Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology Institutions: University of Califonia, Berkeley, California, USA
The neutral oligosaccharides from Saccharomyces cerevisiae mnn1 mnn9, mnn2 mnn9, and mnn9 mutant mannoproteins, and from mnn1 and wild type carboxypeptidase Y, have been characterized. The major oligosaccharide from the mnn1 mnn9 mutant, Man10GlcNAc, has the structure (formula; see text) whereas the largest oligosaccharide from the mnn9 mutant, Man13GlcNAc, has the structure (formula; see text) the differences being due to the mnn1 mutation. The smaller mnn9 homologs had lesser amounts of terminal α 1----3-linked mannose and may be precursors of the mature oligosaccharide. The mnn2 mutation had no effect on the mnn9 oligosaccharide structures. Carboxypeptidase Y and mnn9 oligosaccharides were identical, which suggests that the mnn9 mutation eliminates the differences in carbohydrate structure that distinguish intra- from extracellular mannoproteins. One mnn1 mnn9 oligosaccharide, Man11GlcNAc, retained the terminal α 1----2-linked mannose of the lipid-linked core precursor, which suggests that processing to give the larger oligosaccharides can occur without removal of this unit. A smaller mnn1 mnn9 oligosaccharide, Man9GlcNAc, was a mixture of isomers that must, in part, have arisen by action of an α 1----2-mannosidase.
Methods: gel filtration, 1H NMR, FAB-MS, endoglucosaminidase H digestion, thin layer chromatography Biosynthesis and genetic data: The mnn9 mutation possibly eliminates the differences in carbohydrate structure that distinguish intra- from extracellular mannoproteins. Comments, role: Parent molecule: mannoprotein; the strain used bears a mutation in mnn9
Related record ID(s): 134184 NCBI Taxonomy refs (TaxIDs):4932 Reference(s) to other database(s): GTC:G35180SG, CCSD:11913, CBank-STR:21163 Show glycosyltransferases
There are 2 chemically distinct structures. Please, select:
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