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Kvasnicka F, Price KR, Ng K, Fenwick GR
Determination of potato glycoalkaloids using isotachophoresis and comparison with a HPLC method
Journal of Liquid Chromatography 17 (1994)
1941-1951
Solanum tuberosum
(NCBI TaxID 4113,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: tuber
Publication DOI: 10.1080/10826079408013470Journal NLM ID: 7806595Publisher: Dekker
Institutions: Institute of Chemical Technology, Department of Carbohydrate Chemistry and Technology, Prague, Czech Republic, Institute of Food Research, Food Molecular Biochemistry Department, Norwich Research Park, Colney, Norwich, United Kingdom
A method for the determination of potato glycoalkaloids (PGAs) and their aglycone, solanidine, by capillary isotachophoresis (ITP) was developed. The PGAs were extracted by methanol or by a mixture of methanol : water : acetic acid and then purified by solid phase extraction (SPE). The leading electrolyte consisted of 2 mM HCl solution in 99% methanol and 5 mM Zn(NO3)2 in 99% methanol served as terminating electrolyte. The average recoveries were 88–101% for solanidine and 90–103% for α-PGAs at a level of 50 mg/kg. The detection limit of solanidine and α-PGAs was 1 mg/kg and 2 mg/kg, respectively. A comparison of the ITP and a HPLC method for the determination of PGAs in commercial products was carried out. The HPLC determination of PGAs was carried out on a Nucleosil 5-NH2 column using a mixture of acetonitrile and 20 mM KH2PO4 (75 : 25, v/v) as the mobile phase with UV detection at 208 nm. Comparative studies showed that the methods give similar figures for total glycoalkaloid content (sum of α-solanine and α-chaconine). The HPLC method provided both the separation and quantification of both α-solanine and α-chaconine but this technique under conditions used did not allow the determination of the aglycone solanidine. ITP does not provide the separation of individual glycoalkaloids differing only in sugar moiety such as α-solanine and α-chaconine but does allow the separation and determination of aglycone solanidine and the α-glycoalkaloids in one run.
Structure type: oligomer
Trivial name: solamargine, chacotriose, α-chaconine, solamargine α-chaconine, chaconine, chacotriose solanidine α-chaconine, chacotriose α-chaconine
Compound class: saponin glycoside, glycoside
Contained glycoepitopes: IEDB_136105,IEDB_142488,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: HPLC, capillary isotachophoresis
NCBI Taxonomy refs (TaxIDs): 4113Reference(s) to other database(s): CCSD:
49713, CBank-STR:6366
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Kvasnicka F, Price KR, Ng K, Fenwick GR
Determination of potato glycoalkaloids using isotachophoresis and comparison with a HPLC method
Journal of Liquid Chromatography 17 (1994)
1941-1951
Solanum tuberosum
(NCBI TaxID 4113,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: tuber
Publication DOI: 10.1080/10826079408013470Journal NLM ID: 7806595Publisher: Dekker
Institutions: Institute of Chemical Technology, Department of Carbohydrate Chemistry and Technology, Prague, Czech Republic, Institute of Food Research, Food Molecular Biochemistry Department, Norwich Research Park, Colney, Norwich, United Kingdom
A method for the determination of potato glycoalkaloids (PGAs) and their aglycone, solanidine, by capillary isotachophoresis (ITP) was developed. The PGAs were extracted by methanol or by a mixture of methanol : water : acetic acid and then purified by solid phase extraction (SPE). The leading electrolyte consisted of 2 mM HCl solution in 99% methanol and 5 mM Zn(NO3)2 in 99% methanol served as terminating electrolyte. The average recoveries were 88–101% for solanidine and 90–103% for α-PGAs at a level of 50 mg/kg. The detection limit of solanidine and α-PGAs was 1 mg/kg and 2 mg/kg, respectively. A comparison of the ITP and a HPLC method for the determination of PGAs in commercial products was carried out. The HPLC determination of PGAs was carried out on a Nucleosil 5-NH2 column using a mixture of acetonitrile and 20 mM KH2PO4 (75 : 25, v/v) as the mobile phase with UV detection at 208 nm. Comparative studies showed that the methods give similar figures for total glycoalkaloid content (sum of α-solanine and α-chaconine). The HPLC method provided both the separation and quantification of both α-solanine and α-chaconine but this technique under conditions used did not allow the determination of the aglycone solanidine. ITP does not provide the separation of individual glycoalkaloids differing only in sugar moiety such as α-solanine and α-chaconine but does allow the separation and determination of aglycone solanidine and the α-glycoalkaloids in one run.
Structure type: oligomer
Trivial name: solatriose, α-solanine, solatriose α-solanine, solanine solatriose, solanine
Compound class: saponin glycoside, glycoside, triterpenoid glycoside
Contained glycoepitopes: IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: HPLC, capillary isotachophoresis
NCBI Taxonomy refs (TaxIDs): 4113Reference(s) to other database(s): CCSD:
49712, CBank-STR:8737
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There is only one chemically distinct structure:
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