Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 25107979Publication DOI: 10.1128/AEM.02104-14Journal NLM ID: 7605801Publisher: American Society for Microbiology
Correspondence: sabina.gorska
iitd.pan.wroc.pl; schwarzer
biomed.cas.cz
Institutions: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland, Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology, Novy Hradek, Czech Republic
The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results from stimulation of mouse bone marrow-derived dendritic cells (BM-DC) and human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. rhamnosus LOCK 0900 produces PS that differ greatly in their structure. The polymer L900/2, with a high average molecular mass of 830 kDa, is a branched heteropolysaccharide with a unique repeating unit consisting of seven sugar residues and pyruvic acid, whereas L900/3 has a low average molecular mass of 18 kDa and contains a pentasaccharide repeating unit and phosphorus. Furthermore, we found that both described PS neither induce cytokine production and maturation of mouse BM-DC nor induce signaling through TLR2/TLR4 receptors. However, they differ profoundly in their abilities to modulate the BM-DC immune response to the well-characterized human isolate Lactobacillus plantarum WCFS1. Exposure to L900/2 enhanced interleukin-10 (IL-10) production induced by L. plantarum WCFS1, while in contrast, L900/3 enhanced the production of IL-12p70. We conclude that PS, probably due to their chemical features, are able to modulate the immune responses to third-party antigens. The ability to induce regulatory IL-10 by L900/2 opens up the possibility to use this PS in therapy of inflammatory conditions, such as inflammatory bowel disease, whereas L900/3 might be useful in reverting the antigen-dependent Th2-skewed immune responses in allergies.
Structure type: polymer chemical repeating unit ; 830000, 920000
Location inside paper: Fig. 2
Trivial name: PS L900/2, PS L900/4
Compound class: CPS
Contained glycoepitopes: IEDB_115015,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151770,IEDB_151771,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GLC-MS, GPC, flow citometry
Biological activity: CPS modulates the immune response of BM-DC induced by L. plantarum WCFS1
Biosynthesis and genetic data: biochemical data
Comments, role: R-configuration of Pyr was suggested from the NMR data. C4 and C5 of Man were swapped in the paper.
Related record ID(s): 15051
NCBI Taxonomy refs (TaxIDs): 1316933
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,2,3,3 aDFucp 100.8 66.9 75.0 70.1 68.8 16.5
3,3,3,2,3 aDManp 104.2 74.2 82.2 68.3 75.8 60.9
3,3,3,2 aDFucp 99.7 76.0 78.7 71.1 69.3 16.5
3,3,3 aDGlcp 94.5 77.9 72.8 67.7 69.4 60.2
3,3,6 xRPyr 175.8 101.6 25.0
3,3 aDGalp 98.1 68.0 71.6 71.5 62.4 64.8
3 aDFucp 102.1 69.7 78.4 71.3 69.2 16.5
aDFucp 102.1 69.7 77.9 71.1 69.3 16.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,2,3,3 aDFucp 5.09 4.18 3.88 3.54 3.93 1.106
3,3,3,2,3 aDManp 4.58 3.17 3.53 3.36 3.34 3.64-3.86
3,3,3,2 aDFucp 5.08 4.27 4.02 3.58 3.85 1.225
3,3,3 aDGlcp 5.11 3.62 3.87 3.45 3.93 3.72-3.77
3,3,6 xRPyr - - 1.37
3,3 aDGalp 4.98 3.85 3.93 4.18 4.15 3.81-3.96
3 aDFucp 4.969 4.12 3.88 3.48 3.79 1.224
aDFucp 4.978 4.12 3.79 3.48 3.78 1.236
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,2,3,3 aDFucp 100.8/5.09 66.9/4.18 75.0/3.88 70.1/3.54 68.8/3.93 16.5/1.106
3,3,3,2,3 aDManp 104.2/4.58 74.2/3.17 82.2/3.53 68.3/3.36 75.8/3.34 60.9/3.64-3.86
3,3,3,2 aDFucp 99.7/5.08 76.0/4.27 78.7/4.02 71.1/3.58 69.3/3.85 16.5/1.225
3,3,3 aDGlcp 94.5/5.11 77.9/3.62 72.8/3.87 67.7/3.45 69.4/3.93 60.2/3.72-3.77
3,3,6 xRPyr 25.0/1.37
3,3 aDGalp 98.1/4.98 68.0/3.85 71.6/3.93 71.5/4.18 62.4/4.15 64.8/3.81-3.96
3 aDFucp 102.1/4.969 69.7/4.12 78.4/3.88 71.3/3.48 69.2/3.79 16.5/1.224
aDFucp 102.1/4.978 69.7/4.12 77.9/3.79 71.1/3.48 69.3/3.78 16.5/1.236
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,2,3,3 | aDFucp | 5.09 | 4.18 | 3.88 | 3.54 | 3.93 | 1.106 |
| 3,3,3,2,3 | aDManp | 4.58 | 3.17 | 3.53 | 3.36 | 3.34 | 3.64
3.86 |
| 3,3,3,2 | aDFucp | 5.08 | 4.27 | 4.02 | 3.58 | 3.85 | 1.225 |
| 3,3,3 | aDGlcp | 5.11 | 3.62 | 3.87 | 3.45 | 3.93 | 3.72
3.77 |
| 3,3,6 | xRPyr |
|
| 1.37 | |
| 3,3 | aDGalp | 4.98 | 3.85 | 3.93 | 4.18 | 4.15 | 3.81
3.96 |
| 3 | aDFucp | 4.969 | 4.12 | 3.88 | 3.48 | 3.79 | 1.224 |
| | aDFucp | 4.978 | 4.12 | 3.79 | 3.48 | 3.78 | 1.236 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,2,3,3 | aDFucp | 100.8 | 66.9 | 75.0 | 70.1 | 68.8 | 16.5 |
| 3,3,3,2,3 | aDManp | 104.2 | 74.2 | 82.2 | 68.3 | 75.8 | 60.9 |
| 3,3,3,2 | aDFucp | 99.7 | 76.0 | 78.7 | 71.1 | 69.3 | 16.5 |
| 3,3,3 | aDGlcp | 94.5 | 77.9 | 72.8 | 67.7 | 69.4 | 60.2 |
| 3,3,6 | xRPyr | 175.8 | 101.6 | 25.0 | |
| 3,3 | aDGalp | 98.1 | 68.0 | 71.6 | 71.5 | 62.4 | 64.8 |
| 3 | aDFucp | 102.1 | 69.7 | 78.4 | 71.3 | 69.2 | 16.5 |
| | aDFucp | 102.1 | 69.7 | 77.9 | 71.1 | 69.3 | 16.5 |
|
There is only one chemically distinct structure:
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 25107979Publication DOI: 10.1128/AEM.02104-14Journal NLM ID: 7605801Publisher: American Society for Microbiology
Correspondence: sabina.gorska
iitd.pan.wroc.pl; schwarzer
biomed.cas.cz
Institutions: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland, Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology, Novy Hradek, Czech Republic
The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results from stimulation of mouse bone marrow-derived dendritic cells (BM-DC) and human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. rhamnosus LOCK 0900 produces PS that differ greatly in their structure. The polymer L900/2, with a high average molecular mass of 830 kDa, is a branched heteropolysaccharide with a unique repeating unit consisting of seven sugar residues and pyruvic acid, whereas L900/3 has a low average molecular mass of 18 kDa and contains a pentasaccharide repeating unit and phosphorus. Furthermore, we found that both described PS neither induce cytokine production and maturation of mouse BM-DC nor induce signaling through TLR2/TLR4 receptors. However, they differ profoundly in their abilities to modulate the BM-DC immune response to the well-characterized human isolate Lactobacillus plantarum WCFS1. Exposure to L900/2 enhanced interleukin-10 (IL-10) production induced by L. plantarum WCFS1, while in contrast, L900/3 enhanced the production of IL-12p70. We conclude that PS, probably due to their chemical features, are able to modulate the immune responses to third-party antigens. The ability to induce regulatory IL-10 by L900/2 opens up the possibility to use this PS in therapy of inflammatory conditions, such as inflammatory bowel disease, whereas L900/3 might be useful in reverting the antigen-dependent Th2-skewed immune responses in allergies.
Structure type: polymer chemical repeating unit ; 18000
Location inside paper: Fig. 4
Trivial name: PS L900/3
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_241118,IEDB_885813,IEDB_983931,SB_192,SB_21,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GLC-MS, GPC, flow citometry
Biological activity: CPS modulates the immune response of BM-DC induced by L. plantarum WCFS1
Biosynthesis and genetic data: biochemical data
Related record ID(s): 15050
NCBI Taxonomy refs (TaxIDs): 1316933Reference(s) to other database(s): GTC:G21492MI
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,6 P
3,3 bDGlcp 104.6 72.8 75.7 73.8 75.3 64.8
3,2 Ac ? 21.9
3 bDGalpN 103.3 51.5 80.3 67.9 74.7 60.3
4,6 bDGlcp 102.5 72.9 75.5 69.2 75.9 60.6
4,2 Ac ? 22.1
4 bDManpN 98.6 53.0 71.8 66.8 75.6 69.4
aDGalp 98.2 67.9 77.4 75.5 69.9 61.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,6 P
3,3 bDGlcp 4.46 3.28 3.36 3.52 3.39 3.65-3.95
3,2 Ac - 1.95
3 bDGalpN 4.65 3.97 3.77 4.02 3.56 3.70
4,6 bDGlcp 4.37 3.26 3.37 3.27 3.38 3.63-3.80
4,2 Ac - 1.93
4 bDManpN 5.02 4.43 3.74 3.46 3.26 3.72-4.01
aDGalp 4.81 3.80 3.95 4.26 4.06 3.68
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,6 P
3,3 bDGlcp 104.6/4.46 72.8/3.28 75.7/3.36 73.8/3.52 75.3/3.39 64.8/3.65-3.95
3,2 Ac 21.9/1.95
3 bDGalpN 103.3/4.65 51.5/3.97 80.3/3.77 67.9/4.02 74.7/3.56 60.3/3.70
4,6 bDGlcp 102.5/4.37 72.9/3.26 75.5/3.37 69.2/3.27 75.9/3.38 60.6/3.63-3.80
4,2 Ac 22.1/1.93
4 bDManpN 98.6/5.02 53.0/4.43 71.8/3.74 66.8/3.46 75.6/3.26 69.4/3.72-4.01
aDGalp 98.2/4.81 67.9/3.80 77.4/3.95 75.5/4.26 69.9/4.06 61.0/3.68
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,6 | P | |
| 3,3 | bDGlcp | 4.46 | 3.28 | 3.36 | 3.52 | 3.39 | 3.65
3.95 |
| 3,2 | Ac |
| 1.95 | |
| 3 | bDGalpN | 4.65 | 3.97 | 3.77 | 4.02 | 3.56 | 3.70 |
| 4,6 | bDGlcp | 4.37 | 3.26 | 3.37 | 3.27 | 3.38 | 3.63
3.80 |
| 4,2 | Ac |
| 1.93 | |
| 4 | bDManpN | 5.02 | 4.43 | 3.74 | 3.46 | 3.26 | 3.72
4.01 |
| | aDGalp | 4.81 | 3.80 | 3.95 | 4.26 | 4.06 | 3.68 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,6 | P | |
| 3,3 | bDGlcp | 104.6 | 72.8 | 75.7 | 73.8 | 75.3 | 64.8 |
| 3,2 | Ac | ? | 21.9 | |
| 3 | bDGalpN | 103.3 | 51.5 | 80.3 | 67.9 | 74.7 | 60.3 |
| 4,6 | bDGlcp | 102.5 | 72.9 | 75.5 | 69.2 | 75.9 | 60.6 |
| 4,2 | Ac | ? | 22.1 | |
| 4 | bDManpN | 98.6 | 53.0 | 71.8 | 66.8 | 75.6 | 69.4 |
| | aDGalp | 98.2 | 67.9 | 77.4 | 75.5 | 69.9 | 61.0 |
|
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
report error
Found 2 records.
Displayed records from 1 to 2
(previously named: Lactobacillus casei LOCK 0900)