In the present investigation, we successfully employed a cell-free extract of Esteya vermicola CNU 120806 to convert ginsenoside Rg3 to Rh2. Three important factors including pH, temperature and substrate concentration were optimized for the preparation of Rh 2 . The optimal condition was obtained as follows: 50°C, pH 5.0 and substrate concentration of 3 mg ml -1 . The yield of conversion was up to 90.7%. In order to identify the specificity of the β-glucosidase activity of Esteya vermicola CNU 120806, ginsenoside Re (protopanaxatriol saponins) was treated under the same reaction system. Interestingly, no new metabolite was generated, which elucidated that the enzymatic process only occurred by hydrolysis of the terminal glucopyranosyl moieties at the C-3 carbon of ginsenoside Rg 3 . The crude enzyme extract can be used for commercial ginsenoside Rh 2 preparation.
biotransformation, Esteya vermicola CNU 120806, ginsenoside Rg 3, ginsenoside Rh 2
NCBI PubMed ID: 22805964Publication DOI: 10.1007/s11274-011-0946-5Journal NLM ID: 9012472Correspondence: sungchangkeun@126.com
Institutions: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Taejeon, South Korea, College of Chinese Medicinal Material, Jilin Agricultural University, Jilin, China
Methods: 13C NMR, HPLC, enzymatic digestion, extraction, CC, cell growth, enzymatic assay
Found