Microbial transformation of lithocholic acid (3α-hydroxy-5β-cholanic acid) () by a fungus Cunninghamella blakesleeana ST-22 was studied. An unknown metabolite was detected by using [carboxy-14C] lithocholic acid as substrate. The structure of the new metabolite was determined to be lithocholic acid 3α-O-β-D-glucopyranoside on the basis of mass, infrared, and nuclear magnetic resonance spectroscopies.
glycoside, biotransformation, Cunninghamella blakesleeana, lithocholic acid
Publication DOI: 10.1016/0922-338X(92)90083-7Journal NLM ID: 9000935Institutions: Department of Biotechnology, Faculty of Engineering, Osaka University, Suita-shi, Japan
Methods: 13C NMR, 1H NMR, methylation, IR, TLC, ESI-MS, acid hydrolysis, GC, radiolabeling, HPLC, extraction, optical rotation measurement, acetylation, elemental analysis, reduction, melting point determination, evaporation