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This study investigated the inhibitory effects of oligoporin A on platelet aggregation and the mechanism of its action on downstream signaling molecules. Oligoporin A was isolated from the fruiting bodies of Oligoporus tephroleucus (Polyporaceae). The anti-platelet activities of oligoporin A were studied using rat platelets. The effects of oligoporin A on intracellular Ca(2+) mobilization, ATP release, production of the cyclic nucleotides cAMP and cGMP, extracellular signal-regulated kinase (ERK) 2 phosphorylation, and fibrinogen binding to active integrin α(II)(b)β(3) were assessed. Oligoporin A, but not oligoporins B and C, inhibited collagen-induced platelet aggregation in a concentration-dependent manner. Interestingly, oligoporin A did not affect ADP- and thrombin-induced platelet aggregations, which act on different types of membrane receptors. Granule secretion analysis demonstrated that oligoporin A significantly and dose-dependently reduced collagen-induced ATP release and intracellular Ca(2+) mobilization. Additionally, oligoporin A induced the dynamic increase in cAMP and cGMP. Increased cGMP production was further confirmed by the simultaneous production of nitric oxide. Pretreatment with oligoporin A significantly blocked collagen-induced ERK2 phosphorylation. Finally, oligoporin A vaguely diminished the binding of fibrinogen to its cognate receptor, integrin α(II)(b)β(3). The results indicate that oligoporin A inhibits only collagen-induced platelet aggregation mediated through the modulation of downstream signaling molecules. Oligoporin A may be beneficial against cardiovascular disease provoked by aberrant platelet activation.

Oligoporus tephroleucus, oligoporin A, anti-platelet, ERK, calcium, cAMP

NCBI PubMed ID: 22309049
Publication DOI: 10.3109/09537104.2011.629309
Journal NLM ID: 9208117
Publisher: London: Informa Healthcare
Correspondence: Rhee MH
Institutions: College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea, School of Life Science and Biotechnology, Sungkyunkwan University, Suwon, South Korea, College of Biomedical Science and Engineering, and Regional Research Center, Inje University, Gimhae, South Korea, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea, College of Veterinary Medicine, Chungnam National University, Taejeon, South Korea, Department of Industrial Crops, Korea National College of Agriculture and Fisheries, Hwasung, South Korea
Methods: biological assays, immunoblotting, extraction, CC, determination of NO production, flow cytometry

Mushrooms are ubiquitous in nature and have high nutritional attributes. They have demonstrated diverse biological effects and therefore have been used in treatments of various diseases, including cancer, diabetes, bacterial and viral infections, and ulcer. In particular, polysaccharides, including β-glucan, are considered as the major constituents responsible for the biological activity of mushrooms. Although an overwhelming number of reports have been published on the importance of polysaccharides as immunomodulating agents, not all of the healing properties found in these mushrooms could be fully accounted for. Recently, many research groups have begun investigations on biologically active small-molecular weight compounds in wild mushrooms. In this mini-review, both structural diversity and biological activities of novel bioactive substances from Korean native mushrooms are described.

biological activity, bioactive compounds, Korean native mushrooms, structure diversity

NCBI PubMed ID: 24493936
Publication DOI: 10.5941/MYCO.2013.41.4.171
Journal NLM ID: 100960027
Publisher: Seoul, Korea: Korean Society of Mycology
Correspondence: Yun BS < bsyun@jbnu,ac.kr>
Institutions: Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Korea

New triterpene glucosides, oligoporins A (1), B (2), and C (3), were isolated from the methanolic extract of the fruiting bodies of Oligoporus tephroleucus (Polyporaceae). Their structures were established by spectroscopic methods. These compounds significantly exhibited protective effect to plasmid DNA damage by hydroxyl radical (*OH) generated from the Fenton reaction with hydrogen peroxide and ferrous.

Oligoporus tephroleucus, Fenton reaction, oligoporins, DNA protection

NCBI PubMed ID: 17588752
Publication DOI: 10.1016/j.bmcl.2007.06.034
Journal NLM ID: 9107377
Publisher: Elsevier
Correspondence: Yun BS
Institutions: Functional Metabolomics Research Center, KRIBB, Yuseong, Daejeon, Republic of Korea, Department of Applied Biology, Chungnam National University, Daejeon, Republic of Korea
Methods: 13C NMR, 1H NMR, IR, FAB-MS, UV, optical rotation measurement, HR-ESI-MS, DQF-COSY, HMBC, HMQC, DEPT, COSY