A novel photo protective mycosporine was isolated from the lichenized ascomycete Collema cristatum. Biological activity was measured in terms of protection against UV-B induced membrane destruction and pyrimidine dimer formation in cultured human keratinocytes, and prevention of UV-B induced erythema. It was found that the pure isolated compound prevented UV-B induced cell destruction in a dose-dependent manner, that the compound partially prevented pyrimidine dimer formation and completely prevented UV-B induced erythema when applied to the skin prior to irradiation.
mycosporine, lichenized ascomycete, Collema cristatum, photo protective activity, UV-B
NCBI PubMed ID: 14764094Publication DOI: 10.1111/j.1432-1033.2004.03981.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: Srebnik M
Institutions: Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Hebrew University of Jerusalem, Jerusalem, Israel, Teva Pharmaceutical Industries Ltd, Petach Tikva, Israel, Division of Environment Science, The Fredy and Nadine Herrmann Graduate School of Applied Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel, Department of Dermatology, Hadassah University Hospital, Jerusalem, Israel, Institute of Ecology, Haifa University, Haifa, Israel
Methods: 13C NMR, 1H NMR, NMR-2D, IR, HPLC, UV, extraction, biological assay, HR-FAB-MS, UVB irradiation
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