Cultivation of the fungal strain Eurotium cristatum EN-220, an endophyte obtained from the marine alga Sargassum thunbergii, resulted in the isolation of one new anthraquinone glycoside, 3-O-(α-D-ribofuranosyl)questinol (1) and one new orsellinic acid ester, cristatumside A (2), together with three known aromatic glycosides, 3-O-(α-D-ribofuranosyl)questin (3), isotorachrysone 6-O-α-D-ribofuranoside (4), and asperflavin ribofuranoside (5), as well as three anthraquinone derivatives, asperflavin (6), eurorubrin (7), and (+)-variecolorquinone A (8). The structures of these compounds were determined by extensive analysis of their spectroscopic data, as well as by their comparison with those in the literature. Each of the isolated compounds was evaluated for its antimicrobial activity and brine shrimp lethality.
analysis, anthraquinone glycoside, Eurotium cristatum
Publication DOI: 10.1002/hlca.201300358Journal NLM ID: 2985094RPublisher: Verlag Helvetica Chimica Acta
Correspondence: Wang BG
Institutions: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China, College of Chemistry & Pharmacy, Qingdao Agricultural University, Qingdao, China
Methods: 13C NMR, 1H NMR, TLC, HPLC, extraction, optical rotation measurement, CC, cell growth, HR-ESI-MS, DEPT, COSY, VLC
Found