An examination of the endophytic fungus Trichoderma asperellum A-YMD-9-2 obtained from the marine red alga Gracilaria verrucosa led to the isolation of seven new chromanoid norbisabolane derivatives, trichobisabolins I–L (1–4) and trichaspsides C–E (5–7). Their structures and relative configurations were established on the basis of spectroscopic techniques, mainly including 1D/2D NMR and MS, and the absolute configuration of 1 was assigned by X-ray crystallographic analysis using Cu Kα radiation. All of these isolates feature a 1,9-epoxy ring system, and 5–7 represent the second occurrence of norbisabolane aminoglycosides. Compounds 1–7 exhibited potent inhibition of several marine phytoplankton species.
Trichoderma, sesquiterpene, terpene, bisabolane, norbisabolane
NCBI PubMed ID: 31048011Publication DOI: 10.1016/j.fitote.2019.04.014Journal NLM ID: 16930290RPublisher: Elsevier
Correspondence: Ji N-Y
Institutions: University of Chinese Academy of Sciences, Beijing, China, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China
Methods: 13C NMR, 1H NMR, EI-MS, NMR-2D, IR, DNA sequencing, X-ray, DNA techniques, TLC, biological assays, HPLC, UV, extraction, optical rotation measurement, CC, cell growth, melting point determination, antibacterial assay, evaporation, ECD, HR-EI-MS
Found