Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 37951296Publication DOI: 10.1093/jambio/lxad262Journal NLM ID: 9706280Publisher: Oxford: Blackwell Publishing for the Society for Applied Bacteriology
Correspondence: K.V.H. Prashanth <harish
cftri.res.in>
Institutions: Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru 570020, India, Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysuru 570020, India
Aim: To evaluate the structure and functions of capsular exopolysaccharide (CPS) from Bifidobacterium breve NCIM 5671. Methods and results: A CPS produced by the probiotic bacteria B. breve NCIM 5671 was isolated and subjected to characterization through GC analysis, which indicated the presence of rhamnose, fucose, galactose, and glucose in a molar ratio of 3:1:5:3. The average molecular weight of the CPS was determined to be ∼8.5 × 105 Da. Further, NMR analysis revealed the probable CPS structure to be composed of major branched tetra- and penta-saccharide units alternately repeating and having both α- and β-configuration sugar residues. CPS displayed an encouraging prebiotic score for some of the studied probiotic bacteria. Compared to standard inulin, CPS showed better resistance to digestibility against human GI tract in vitro. DPPH, total antioxidant, and ferric reducing assays carried out for CPS displayed decent antioxidant activity too. Conclusion: This study indicates that the CPS from B. breve NCIM 5671 has the potential to be utilized as a prebiotic food supplement. It is a high-molecular-weight (∼8.5 × 105 Da) capsular heteropolysaccharide containing rhamnose, fucose, galactose, and glucose.
fucose, Antioxidant activity, Bifidobacterium breve, capsular exopolysaccharide, prebiotic property
Structure type: structural motif or average structure ; 850000
Location inside paper: table 1, Fig. 3(b)
Compound class: CPS
Methods: 13C NMR, 1H NMR, sugar analysis, GLC, GC, FTIR, HPLC, HPSEC, extraction, statistical analysis, SEM, antioxidant activity assay, prebiotic activity
Comments, role: NMR temperature was not specified; published NMR spectra were removed by CSDB staff due to multiple errors in the NMR assignment
NCBI Taxonomy refs (TaxIDs): 1685
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,4,3,4,4,3,6 bDGlcp 4.59 3.74 4.13 3.48 3.94 3.81
3,4,4,3,4,4,3 aDGalp 5.30 4.06 3.31 3.39 3.41 4.19
3,4,4,3,4,4 aLRhap 5.26 3.87 4.25 3.48 3.74 1.19
3,4,4,3,4 bDGalp 5.14 3.66 3.41 3.87 3.94 3.79
3,4,4,3,6,2 aLFucp 5.19 3.31 3.48 3.79 3.66 1.25
3,4,4,3,6 bDGlcp 4.59 4.25 4.13 3.48 3.94 3.81
3,4,4,3 aDGalp 5.30 4.06 3.31 3.39 3.41 4.19
3,4,4 aLRhap 5.26 3.87 4.25 3.48 3.74 1.19
3,4 bDGalp 5.14 3.66 3.41 3.87 3.94 3.79
3,6 bDGlcp 4.59 3.74 4.13 3.48 3.94 3.81
3 aDGalp 5.30 4.06 3.31 3.39 3.41 4.19
aLRhap 5.26 3.87 4.25 3.48 3.74 1.19
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,4,3,4,4,3,6 | bDGlcp | 4.59 | 3.74 | 4.13 | 3.48 | 3.94 | 3.81 |
| 3,4,4,3,4,4,3 | aDGalp | 5.30 | 4.06 | 3.31 | 3.39 | 3.41 | 4.19 |
| 3,4,4,3,4,4 | aLRhap | 5.26 | 3.87 | 4.25 | 3.48 | 3.74 | 1.19 |
| 3,4,4,3,4 | bDGalp | 5.14 | 3.66 | 3.41 | 3.87 | 3.94 | 3.79 |
| 3,4,4,3,6,2 | aLFucp | 5.19 | 3.31 | 3.48 | 3.79 | 3.66 | 1.25 |
| 3,4,4,3,6 | bDGlcp | 4.59 | 4.25 | 4.13 | 3.48 | 3.94 | 3.81 |
| 3,4,4,3 | aDGalp | 5.30 | 4.06 | 3.31 | 3.39 | 3.41 | 4.19 |
| 3,4,4 | aLRhap | 5.26 | 3.87 | 4.25 | 3.48 | 3.74 | 1.19 |
| 3,4 | bDGalp | 5.14 | 3.66 | 3.41 | 3.87 | 3.94 | 3.79 |
| 3,6 | bDGlcp | 4.59 | 3.74 | 4.13 | 3.48 | 3.94 | 3.81 |
| 3 | aDGalp | 5.30 | 4.06 | 3.31 | 3.39 | 3.41 | 4.19 |
| | aLRhap | 5.26 | 3.87 | 4.25 | 3.48 | 3.74 | 1.19 |
|
13C NMR data: present in publication with incorrect assignment
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
