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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)

The structure was elucidated in this paper
NCBI PubMed ID: 38042318
Publication DOI: 10.1016/j.ijbiomac.2023.128438
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: F. Sha <fengsha

pku.edu.cn>; L. Sun <sunl

njtech.edu.cn>
Institutions: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China, Suzhou Cornigs Polyols CO., LTD., Suzhou 215000, China, Westa College, Southwest University, Chongqing 400715, China, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China

We here describe the isolation of a novel exopolysaccharide from Acinetobacter rhizosphaerae, named ArEPS. The structure of ArEPS was characterized by analysis of the monosaccharide composition, molecular weight, infrared spectrum, methylation, and nuclear magnetic resonance spectrum. ArEPS was found to be an acidic heteropolysaccharide composed of glucose, galactose, galacturonic acid, glucuronic acid, mannose, and glucosamine; the molecular weight was 1533 kDa. Structural analysis showed that the main-chain structure of ArEPS predominantly comprised 1,3,6-β-Glcp, 1,3,4-α-Galp, 1,2-β-Glcp, 1,4-β-GlcpA, 1,4-β-GalpA, and the side-chain structure comprised 1,6-β-Glcp, 1,3-β-Galp, 1-α-Glcp, 1-β-Galp, 1-α-Manp, 1,4,6-α-Glcp, 1,2,4-β-Glcp, 1,2,3-β-Glcp, and 1,3-β-GlcpN. ArEPS significantly enhanced the tolerance of rice seedlings to salt stress. Specifically, plant height, fresh weight, chlorophyll content, and the K+/Na+ ratio increased by 51 %, 63 %, 29 %, and 162 %, respectively, and the malondialdehyde content was reduced by 45 % after treatment with 100 mg/kg ArEPS compared to treatment with 100 mM NaCl. Finally, based on the quadratic regression between fresh weight and ArEPS addition, the optimal ArEPS addition level was estimated to be 135.12 mg/kg. These results indicate the prospects of ArEPS application in agriculture.

exopolysaccharides, Structural characterization, salt stress alleviation

Structure type: structural motif or average structure ; 1533000
Location inside paper: table 2, Fig. 4, ArEPS
Compound class: EPS

Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, FTIR, composition analysis, HPLC, statistical analysis, salt stress, HPGFC-MALLS-RI
Comments, role: published 13C NMR spectra were removed by CSDB staff due to multiple errors in the NMR assignment

Related record ID(s): 24853
NCBI Taxonomy refs (TaxIDs): 243922
Show glycosyltransferases

NMR conditions: in D2O at 298 K [as TSV]

1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
2,4,4,6	bDGlcpA	4.40	3.48	3.60	4.06	3.70	-
2,4,4,3,3,2,2	aDGlcp	4.87	3.82	3.57	3.33	3.63	3.85
2,4,4,3,3,2,4	bDGalp	4.39	3.46	3.38	3.67	3.90	3.67
2,4,4,3,3,2	bDGlcp	4.51	3.70	3.59	4.07	3.72	3.65
2,4,4,3,3,3	ANY
2,4,4,3,3	bDGlcp	4.62	3.44	4.15	3.39	3.67	3.90
2,4,4,3	bDGlcpN	4.58	3.58	3.67	3.60	3.45	3.65-3.66
2,4,4	bDGlcp	4.63	3.43	4.11	3.65	3.38	3.80
2,4,3,4,3,6,4	ANY
2,4,3,4,3,6,6	aDManp	5.33	4.07	3.81	3.59	3.48	3.71
2,4,3,4,3,6	aDGlcp	5.23	4.02	3.73	4.07	3.71	4.28
2,4,3,4,3	bDGlcp	4.57	3.68	3.90	3.61	3.45	4.15
2,4,3,4	bDGalp	4.65	3.42	4.10	3.49	3.36	3.40
2,4,3	bDGalpA	4.88	3.36	3.71	4.10	3.57	-
2,4	aDGalp	5.33	4.07	4.42	4.20	3.84	3.69
2,3,4,3,6,4	ANY
2,3,4,3,6,6	aDManp	5.33	4.07	3.81	3.59	3.48	3.71
2,3,4,3,6	aDGlcp	5.23	4.02	3.73	4.07	3.71	4.28
2,3,4,3	bDGlcp	4.57	3.68	3.90	3.61	3.45	4.15
2,3,4	bDGalp	4.65	3.42	4.10	3.49	3.36	3.40
2,3	bDGalpA	4.88	3.36	3.71	4.10	3.57	-
2	aDGalp	5.33	4.07	4.42	4.20	3.84	3.69
	bDGlcp	5.33	4.07	3.59	3.36	3.66	3.91

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
2,4,4,6	bDGlcpA	4.40	3.48	3.60	4.06	3.70
2,4,4,3,3,2,2	aDGlcp	4.87	3.82	3.57	3.33	3.63	3.85
2,4,4,3,3,2,4	bDGalp	4.39	3.46	3.38	3.67	3.90	3.67
2,4,4,3,3,2	bDGlcp	4.51	3.70	3.59	4.07	3.72	3.65
2,4,4,3,3,3	ANY
2,4,4,3,3	bDGlcp	4.62	3.44	4.15	3.39	3.67	3.90
2,4,4,3	bDGlcpN	4.58	3.58	3.67	3.60	3.45	3.65 3.66
2,4,4	bDGlcp	4.63	3.43	4.11	3.65	3.38	3.80
2,4,3,4,3,6,4	ANY
2,4,3,4,3,6,6	aDManp	5.33	4.07	3.81	3.59	3.48	3.71
2,4,3,4,3,6	aDGlcp	5.23	4.02	3.73	4.07	3.71	4.28
2,4,3,4,3	bDGlcp	4.57	3.68	3.90	3.61	3.45	4.15
2,4,3,4	bDGalp	4.65	3.42	4.10	3.49	3.36	3.40
2,4,3	bDGalpA	4.88	3.36	3.71	4.10	3.57
2,4	aDGalp	5.33	4.07	4.42	4.20	3.84	3.69
2,3,4,3,6,4	ANY
2,3,4,3,6,6	aDManp	5.33	4.07	3.81	3.59	3.48	3.71
2,3,4,3,6	aDGlcp	5.23	4.02	3.73	4.07	3.71	4.28
2,3,4,3	bDGlcp	4.57	3.68	3.90	3.61	3.45	4.15
2,3,4	bDGalp	4.65	3.42	4.10	3.49	3.36	3.40
2,3	bDGalpA	4.88	3.36	3.71	4.10	3.57
2	aDGalp	5.33	4.07	4.42	4.20	3.84	3.69
	bDGlcp	5.33	4.07	3.59	3.36	3.66	3.91

¹³C NMR data: present in publication with incorrect assignment

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](C(=O)O)O[C@@H](OC[C@H]2O[C@@H](O[C@@H]3[C@@H](C(=O)O)O[C@@H](OC[C@H]4O[C@@H](O[C@H]5[C@@H](CO)O[C@H](O[C@H]6[C@@H](CO)O[C@H](O[C@H]7[C@@H](CO)O[C@H](O[C@H]8[C@@H](CO)O[C@H](O[C@H]9[C@H]([*])O[C@H](CO)[C@@H](O)[C@@H]9O)[C@H](O)[C@H]8O[C@@H]8O[C@H](C(=O)O)[C@H](O[C@@H]9O[C@H](CO)[C@H](O)[C@H](O[C@@H]%10O[C@H](CO[C@H]%11O[C@H](CO[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%12O)[C@@H](O[1*])[C@H](O)[C@H]%11O)[C@@H](O)[C@H](O)[C@H]%10O)[C@H]9O)[C@H](O)[C@H]8O)[C@H](O)[C@H]7O[C@@H]7O[C@H](C(=O)O)[C@H](O[C@@H]8O[C@H](CO)[C@H](O)[C@H](O[C@@H]9O[C@H](CO[C@H]%10O[C@H](CO[C@H]%11O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%11O)[C@@H](O[1*])[C@H](O)[C@H]%10O)[C@@H](O)[C@H](O)[C@H]9O)[C@H]8O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O[C@@H]6O[C@H](C(=O)O)[C@H](O[C@@H]7O[C@H](CO)[C@H](O)[C@H](O[C@@H]8O[C@H](CO[C@H]9O[C@H](CO[C@H]%10O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%10O)[C@@H](O[1*])[C@H](O)[C@H]9O)[C@@H](O)[C@H](O)[C@H]8O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O[C@@H]5O[C@H](C(=O)O)[C@H](O[C@@H]6O[C@H](CO)[C@H](O)[C@H](O[C@@H]7O[C@H](CO[C@H]8O[C@H](CO[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]9O)[C@@H](O[1*])[C@H](O)[C@H]8O)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O[1*])[C@H]6O[C@@H]6O[C@H](CO)[C@@H](O[C@@H]7O[C@H](CO)[C@H](O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@H]5N)[C@@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O[1*])[C@H]4O[C@@H]4O[C@H](CO)[C@@H](O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3N)[C@@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

R1 = ANY

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11: XN8LS

]

NCBI PubMed ID: 38042318
Publication DOI: 10.1016/j.ijbiomac.2023.128438
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: F. Sha <fengsha

pku.edu.cn>; L. Sun <sunl

exopolysaccharides, Structural characterization, salt stress alleviation

Structure type: polymer chemical repeating unit
Location inside paper: p. 128438-6
Compound class: EPS, CPS
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_147450,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_23,SB_24,SB_7,SB_8,SB_88

Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, FTIR, composition analysis, HPLC, statistical analysis, salt stress, HPGFC-MALLS-RI
Comments, role: minor repeat units

Related record ID(s): 22322
NCBI Taxonomy refs (TaxIDs): 470
Reference(s) to other database(s): GTC:G89410LL
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](NC(C)=O)[C@H](O[C@@H]2[C@@H](O)[C@H]([*])O[C@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)O[C@H](CO)[C@@H]1O

SVG MW SMILES 3D mol Ionize