Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
NCBI PubMed ID: 37196715Publication DOI: 10.1016/j.ijbiomac.2023.124899Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: L. Sun <1000004354
ujs.edu.cn>
Institutions: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Gellan gum (GG) is used in many industries. Here, we obtained a low molecular weight GG (L-GG) directly produced by M155, the high-yield mutant strain of Sphingomonas paucimobilis ATCC 31461, which was selected using UV-ARTP combined mutagenesis. The molecular weight of L-GG was 44.6 % lesser than that of the initial GG (I-GG), and the GG yield increased by 24 %. The monosaccharide composition and Fourier transform-infrared spectroscopic patterns of L-GG were similar to those of I-GG, which indicated that the decrease in the molecular weight of L-GG was probably because of reduction in the degree of polymerization. In addition, microstructural analysis revealed that the surface of L-GG was rougher, with smaller pores and tighter network, than that of I-GG. L-GG showed low hardness, gumminess, and chewiness, which are indicative of better taste. The results of rheological analysis revealed that the L-GG solution is a typical non-Newtonian fluid with low viscoelasticity, which exhibited stable dynamic viscoelasticity within 20-65 °C. To the best of our knowledge, this is the first report of direct biosynthesis of low molecular weight GG during fermentation, which will reduce the manufacturing costs. Our observations provide a reference for precise and expanded applications of GG.
physicochemical properties, Gellan gum, low molecular weight
Structure type: structural motif or average structure
Location inside paper: graphical abstract, p. 124899-1, low-molecular weight gellan gum (L-GG)
Trivial name: gellan, sphingan, gellan gum, gellan gum (GG)
Compound class: EPS, O-polysaccharide, rhamnoglucan
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_189517,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983931,SB_192
Methods: HPAEC, FTIR, statistical analysis, SEC-MALLS, rheological study, SEM, WHC, UV-ARTP combined mutagenesis, mutant screening
NCBI Taxonomy refs (TaxIDs): 1081640Reference(s) to other database(s): GTC:G22552GU
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Sphingomonas elodea ATCC 31461)