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Mishra AK, Alderwick LJ, Rittmann D, Wang C, Bhatt A, Jacobs WR, Takayama K, Eggeling L, Besra GS
Identification of a novel α(1→6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant
Molecular Microbiology 68(6) (2008)
1595-1613
Corynebacterium glutamicum NCgl1505
(Ancestor NCBI TaxID 1718,
species name lookup)
Mycobacterium smegmatis
(NCBI TaxID 1772,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Organ / tissue: cell wall
NCBI PubMed ID: 18452585Publication DOI: 10.1111/j.1365-2958.2008.06265.xJournal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: g.besra
bham.ac.uk;
Institutions: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Mycobacterium tuberculosis and Corynebacterium glutamicum share a similar cell wall structure and orthologous enzymes involved in cell wall assembly. Herein, we have studied C. glutamicum NCgl1505, the orthologue of putative glycosyltransferases Rv1459c from M. tuberculosis and MSMEG3120 from Mycobacterium smegmatis. Deletion of NCgl1505 resulted in the absence of lipomannan (Cg-LM-A), lipoarabinomannan (Cg-LAM) and a multi-mannosylated polymer (Cg-LM-B) based on a 1,2-di-O-C(16)/C(18:1)-(α-D-glucopyranosyluronic acid)-(1→3)-glycerol (GlcAGroAc(2)) anchor, while syntheses of triacylated-phosphatidyl-myo-inositol dimannoside (Ac(1)PIM(2)) and Man(1)GlcAGroAc(2) were still abundant in whole cells. Cell-free incubation of C. glutamicum membranes with GDP-[(14)C]Man established that C. glutamicum synthesized a novel α(1→6)-linked linear form of Cg-LM-A and Cg-LM-B from Ac(1)PIM(2) and Man(1)GlcAGroAc(2) respectively. Furthermore, deletion of NCgl1505 also led to the absence of in vitro synthesized linear Cg-LM-A and Cg-LM-B, demonstrating that NCgl1505 was involved in core α(1→6) mannan biosynthesis of Cg-LM-A and Cg-LM-B, extending Ac(1)PI[(14)C]M(2) and [(14)C]Man(1)GlcAGroAc(2) primers respectively. Use of the acceptor α-D-Manp-(1→6)-α-D-Manp-O-C(8) in an in vitro cell-free assay confirmed NCgl1505 as an α(1→6) mannopyranosyltransferase, now termed MptB. While Rv1459c and MSMEG3120 demonstrated similar in vitro α(1→6) mannopyranosyltransferase activity, deletion of the Rv1459c homologue in M. smegmatis did not result in loss of mycobacterial LM/LAM, indicating a functional redundancy for this enzyme in mycobacteria.
biosynthesis, gene, cell wall, lipoarabinomannan, Mycobacterium tuberculosis, Mycobacterium smegmatis, anchor, Corynebacterium glutamicum, mannopyranosyltransferase MptB
Structure type: oligomer
Location inside paper: p.1603, fig.5A
Aglycon: C8
Trivial name: synthetic acceptor
Contained glycoepitopes: IEDB_130701,IEDB_141793,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: TLC, serological methods, genetic methods, biochemical methods, acetolysis, SDS-PAGE/autoradiography
Biosynthesis and genetic data: genetic data
Synthetic data: enzymatic
Related record ID(s): 23161, 23162
NCBI Taxonomy refs (TaxIDs): 1718,
1772Reference(s) to other database(s): GTC:G77384RA, GlycomeDB:
281
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Mishra AK, Alderwick LJ, Rittmann D, Wang C, Bhatt A, Jacobs WR, Takayama K, Eggeling L, Besra GS
Identification of a novel α(1→6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant
Molecular Microbiology 68(6) (2008)
1595-1613
Corynebacterium glutamicum NCgl1505
(Ancestor NCBI TaxID 1718,
species name lookup)
Mycobacterium smegmatis
(NCBI TaxID 1772,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 18452585Publication DOI: 10.1111/j.1365-2958.2008.06265.xJournal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: g.besra
bham.ac.uk;
Institutions: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Mycobacterium tuberculosis and Corynebacterium glutamicum share a similar cell wall structure and orthologous enzymes involved in cell wall assembly. Herein, we have studied C. glutamicum NCgl1505, the orthologue of putative glycosyltransferases Rv1459c from M. tuberculosis and MSMEG3120 from Mycobacterium smegmatis. Deletion of NCgl1505 resulted in the absence of lipomannan (Cg-LM-A), lipoarabinomannan (Cg-LAM) and a multi-mannosylated polymer (Cg-LM-B) based on a 1,2-di-O-C(16)/C(18:1)-(α-D-glucopyranosyluronic acid)-(1→3)-glycerol (GlcAGroAc(2)) anchor, while syntheses of triacylated-phosphatidyl-myo-inositol dimannoside (Ac(1)PIM(2)) and Man(1)GlcAGroAc(2) were still abundant in whole cells. Cell-free incubation of C. glutamicum membranes with GDP-[(14)C]Man established that C. glutamicum synthesized a novel α(1→6)-linked linear form of Cg-LM-A and Cg-LM-B from Ac(1)PIM(2) and Man(1)GlcAGroAc(2) respectively. Furthermore, deletion of NCgl1505 also led to the absence of in vitro synthesized linear Cg-LM-A and Cg-LM-B, demonstrating that NCgl1505 was involved in core α(1→6) mannan biosynthesis of Cg-LM-A and Cg-LM-B, extending Ac(1)PI[(14)C]M(2) and [(14)C]Man(1)GlcAGroAc(2) primers respectively. Use of the acceptor α-D-Manp-(1→6)-α-D-Manp-O-C(8) in an in vitro cell-free assay confirmed NCgl1505 as an α(1→6) mannopyranosyltransferase, now termed MptB. While Rv1459c and MSMEG3120 demonstrated similar in vitro α(1→6) mannopyranosyltransferase activity, deletion of the Rv1459c homologue in M. smegmatis did not result in loss of mycobacterial LM/LAM, indicating a functional redundancy for this enzyme in mycobacteria.
biosynthesis, gene, cell wall, lipoarabinomannan, Mycobacterium tuberculosis, Mycobacterium smegmatis, anchor, Corynebacterium glutamicum, mannopyranosyltransferase MptB
Structure type: oligomer
Location inside paper: p.1603, fig.5A, Product X
Aglycon: C8
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: TLC, serological methods, genetic methods, biochemical methods, acetolysis, SDS-PAGE/autoradiography
Enzymes that release or process the structure: a(1-6)mannopyranosyltransferase MptB/A
Biosynthesis and genetic data: genetic data
Synthetic data: enzymatic
Related record ID(s): 22704, 23162
NCBI Taxonomy refs (TaxIDs): 1718,
1772Reference(s) to other database(s): GTC:G85415LL, GlycomeDB:
2240
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There is only one chemically distinct structure:
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Mishra AK, Alderwick LJ, Rittmann D, Wang C, Bhatt A, Jacobs WR, Takayama K, Eggeling L, Besra GS
Identification of a novel α(1→6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant
Molecular Microbiology 68(6) (2008)
1595-1613
Corynebacterium glutamicum NCgl1505
(Ancestor NCBI TaxID 1718,
species name lookup)
Mycobacterium smegmatis
(NCBI TaxID 1772,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 18452585Publication DOI: 10.1111/j.1365-2958.2008.06265.xJournal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: g.besra
bham.ac.uk;
Institutions: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Mycobacterium tuberculosis and Corynebacterium glutamicum share a similar cell wall structure and orthologous enzymes involved in cell wall assembly. Herein, we have studied C. glutamicum NCgl1505, the orthologue of putative glycosyltransferases Rv1459c from M. tuberculosis and MSMEG3120 from Mycobacterium smegmatis. Deletion of NCgl1505 resulted in the absence of lipomannan (Cg-LM-A), lipoarabinomannan (Cg-LAM) and a multi-mannosylated polymer (Cg-LM-B) based on a 1,2-di-O-C(16)/C(18:1)-(α-D-glucopyranosyluronic acid)-(1→3)-glycerol (GlcAGroAc(2)) anchor, while syntheses of triacylated-phosphatidyl-myo-inositol dimannoside (Ac(1)PIM(2)) and Man(1)GlcAGroAc(2) were still abundant in whole cells. Cell-free incubation of C. glutamicum membranes with GDP-[(14)C]Man established that C. glutamicum synthesized a novel α(1→6)-linked linear form of Cg-LM-A and Cg-LM-B from Ac(1)PIM(2) and Man(1)GlcAGroAc(2) respectively. Furthermore, deletion of NCgl1505 also led to the absence of in vitro synthesized linear Cg-LM-A and Cg-LM-B, demonstrating that NCgl1505 was involved in core α(1→6) mannan biosynthesis of Cg-LM-A and Cg-LM-B, extending Ac(1)PI[(14)C]M(2) and [(14)C]Man(1)GlcAGroAc(2) primers respectively. Use of the acceptor α-D-Manp-(1→6)-α-D-Manp-O-C(8) in an in vitro cell-free assay confirmed NCgl1505 as an α(1→6) mannopyranosyltransferase, now termed MptB. While Rv1459c and MSMEG3120 demonstrated similar in vitro α(1→6) mannopyranosyltransferase activity, deletion of the Rv1459c homologue in M. smegmatis did not result in loss of mycobacterial LM/LAM, indicating a functional redundancy for this enzyme in mycobacteria.
biosynthesis, gene, cell wall, lipoarabinomannan, Mycobacterium tuberculosis, Mycobacterium smegmatis, anchor, Corynebacterium glutamicum, mannopyranosyltransferase MptB
Structure type: oligomer
Location inside paper: p.1603, fig.5A, Product Y
Aglycon: C8
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: TLC, serological methods, genetic methods, biochemical methods, acetolysis, SDS-PAGE/autoradiography
Enzymes that release or process the structure: a(1-6)mannopyranosyltransferase MptB/A
Biosynthesis and genetic data: genetic data
Synthetic data: enzymatic
Related record ID(s): 22704, 23161
NCBI Taxonomy refs (TaxIDs): 1718,
1772Reference(s) to other database(s): GTC:G03889QT, GlycomeDB:
37243
Show glycosyltransferases
There is only one chemically distinct structure:
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