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Karas M, Russa R
Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
Acta Biochimica Polonica 56(1) (2009)
155-160
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Subst2-(?-8)-+ Subst2-(?-8)-+
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Subst1-(1-4)-b-D-GlcpNAc-(1-4)-b-Murp2Ac-(1-4)-b-D-GlcpNAc-(1-4)-b-Murp2Ac-(1-?)-Subst3
Subst1 = peptidoglycan;
Subst2 = side peptide chain;
Subst3 = oligoglucan |
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Mesorhizobium loti HAMBI 1148
(Ancestor NCBI TaxID 381,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 19294234Journal NLM ID: 14520300RPublisher: Panstwowe Wydawnictwo Naukowe
Correspondence: Ryszard.Russa
poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland
The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H](-) or [M+Na-2H](-) ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
MALDI-TOF, deamination, murein, Mesorhizobium, oligoglucan
Structure type: oligomer
Location inside paper: p.157, fig.1
Trivial name: murein glycan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_1635957,IEDB_423183,IEDB_885814
Methods: partial acid hydrolysis, GC-MS, sugar analysis, MALDI-TOF MS, de-N-acetylation, alkaline de-N-acylation, enzymatic digestion, lactamization with hydrazine acetate
Comments, role: oligoglucan: Oligoglucan: Glcn, n = 1 or 3 glucose residues.
Related record ID(s): 24017, 24018, 24019
NCBI Taxonomy refs (TaxIDs): 381
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There is only one chemically distinct structure:
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Karas M, Russa R
Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
Acta Biochimica Polonica 56(1) (2009)
155-160
Mesorhizobium loti HAMBI 1148
(Ancestor NCBI TaxID 381,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 19294234Journal NLM ID: 14520300RPublisher: Panstwowe Wydawnictwo Naukowe
Correspondence: Ryszard.Russa
poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland
The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H](-) or [M+Na-2H](-) ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
MALDI-TOF, deamination, murein, Mesorhizobium, oligoglucan
Structure type: monomer
Location inside paper: p.157, fig.1
Methods: partial acid hydrolysis, GC-MS, sugar analysis, MALDI-TOF MS, de-N-acetylation, alkaline de-N-acylation, enzymatic digestion, lactamization with hydrazine acetate
Comments, role: product of the degradation of murein. in the paper it is referred as 2,5-anhydromannose, but depicted as 2,5-anhydromannitol
Related record ID(s): 23733, 24018, 24019
NCBI Taxonomy refs (TaxIDs): 381Reference(s) to other database(s): GTC:G68961XX
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There is only one chemically distinct structure:
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Karas M, Russa R
Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
Acta Biochimica Polonica 56(1) (2009)
155-160
Mesorhizobium loti HAMBI 1148
(Ancestor NCBI TaxID 381,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 19294234Journal NLM ID: 14520300RPublisher: Panstwowe Wydawnictwo Naukowe
Correspondence: Ryszard.Russa
poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland
The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H](-) or [M+Na-2H](-) ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
MALDI-TOF, deamination, murein, Mesorhizobium, oligoglucan
Structure type: oligomer
Location inside paper: p.157, fig.1
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: partial acid hydrolysis, GC-MS, sugar analysis, MALDI-TOF MS, de-N-acetylation, alkaline de-N-acylation, enzymatic digestion, lactamization with hydrazine acetate
Comments, role: product of the degradation of murein
Related record ID(s): 23733, 24017, 24019
NCBI Taxonomy refs (TaxIDs): 381
Show glycosyltransferases
There is only one chemically distinct structure:
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Karas M, Russa R
Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
Acta Biochimica Polonica 56(1) (2009)
155-160
Mesorhizobium loti HAMBI 1148
(Ancestor NCBI TaxID 381,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 19294234Journal NLM ID: 14520300RPublisher: Panstwowe Wydawnictwo Naukowe
Correspondence: Ryszard.Russa
poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland
The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H](-) or [M+Na-2H](-) ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
MALDI-TOF, deamination, murein, Mesorhizobium, oligoglucan
Structure type: oligomer
Location inside paper: p.157, fig.1
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: partial acid hydrolysis, GC-MS, sugar analysis, MALDI-TOF MS, de-N-acetylation, alkaline de-N-acylation, enzymatic digestion, lactamization with hydrazine acetate
Comments, role: Product of the degradation of murein. Oligoglucan: Glcn, n = 1 or 3 glucose residues.
Related record ID(s): 23733, 24017, 24018
NCBI Taxonomy refs (TaxIDs): 381
Show glycosyltransferases
There is only one chemically distinct structure:
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