Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 19281967Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: k.D. McReynolds <kdmcr
csus.edu>
Institutions: Department of Chemistry, California State University, Sacramento, CA 95819-6057, USA
The hydrolysis of colominic acid via microwave irradiation was studied for the production of short-chain oligomers with a degree of polymerization (DP) of 1-5. This method was compared to the traditional acid hydrolytic method for the production of preparative quantities of short colominic acid oligomers. The oligomers were purified by size exclusion chromatography and characterized by (1)H NMR. Optimal conditions for producing the dimer were found to be 12 min at 10% power in a 1000-Watt domestic microwave. This method is advantageous over the traditional technique in that the hydrolysis can be completed in just a few minutes, rather than in a few hours, it is reproducible, and yields large quantities of the desirable short chain oligomers of colominic acid.
polysaccharides, colominic acid, acid hydrolysis, microwave irradiation, polysialic cid
Structure type: homopolymer ; n=~98
Location inside paper: p.821
Trivial name: colominic acid
Compound class: CPS
Contained glycoepitopes: IEDB_136794,IEDB_141115,IEDB_142352,IEDB_146100,IEDB_149174,IEDB_150072,IEDB_150937,IEDB_153199,IEDB_226810,IEDB_558870,IEDB_983929,SB_170,SB_171,SB_172,SB_35,SB_42,SB_84
Methods: 1H NMR, acid hydrolysis, microwave irradiation
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G30588ZL, GlycomeDB:
677
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error