Phytochemical analysis of the underground parts of Reineckea carnea resulted in the finding of twelve steroidal constituents, five of which appeared to be new compounds and one to be the first isolation from a natural source. The identification and structural determination of the steroidal compounds were established by spectroscopic analysis and hydrolysis. The isolated saponins were evaluated for inhibitory activity on cAMP phosphodiesterase as a primary screening test to find new medicinal materials.
Liliaceae, steroidal saponin, cAMP phosphodiesterase inhibition, Reineckea carnea, steroidal sapogenin
Journal NLM ID: 0377775WWW link: http://ci.nii.ac.jp/naid/110003631056Publisher: Pharmaceutical Society Of Japan
Institutions: Tokyo College of Pharmacy, Tokyo, Japan, School of Pharmaceutical Sciences, Toho University, Japan
Methods: 13C NMR, 1H NMR, IR, TLC, acid hydrolysis, HPLC, enzymatic digestion, CC, biological assay, acetylation analysis