Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 22418092Publication DOI: 10.1016/j.carres.2012.02.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: N.V. Potekhina <potekhina56
mail.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russian Federation
The investigated cell wall polysaccharide of Arthrobacter sp. VKM Ac-2576 (phylum Actinobacteria) is a (1→6)-linked β-D-galactofuranan with the α-D-GlcpNAc3NAc residues that glycosylate virtually all (~90%) hydroxyl groups at C-2 of galactose. Its repeating unit has the following structure: →6)D-β-Galf-(1→2) ↑ α-D-GlcpNAc3NAc-(1 (90%). The structure of the polysaccharide was established by chemical and NMR spectroscopic methods; it is identified for the first time.
polysaccharide, NMR spectroscopy, cell wall, Arthrobacter, galactofuranan
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.217
Trivial name: galactofuranan
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_137472,IEDB_149176,IEDB_190606
Methods: 13C NMR, 1H NMR, sugar analysis, GLC, paper chromatography, paper electrophoresis, acid hydrolysids
Comments, role: NMR data for -6)bDGalf(1-: 1H 5.06 4.13 4.06 4.00 3.96 3.87-3.81, 13C 109.1 82.3 78.2 84.5 70.8 70.4
NCBI Taxonomy refs (TaxIDs): 1667Reference(s) to other database(s): GTC:G33079KO
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,2 Ac 176.1 23.3
2 90%aDGlcpN3N 97.3 52.8 53.3 68.9 74.0 61.7
3 Ac 175.4 23.0
bDGalf 107.3 87.5 76.7 83.7 70.4 70.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,2 Ac - 1.9
2 90%aDGlcpN3N 5.03 4.06 4.13 3.58 3.86 3.82-3.86
3 Ac - 1.98
bDGalf 5.12 4.20 4.28 4.02 4.01 3.64-3.90
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,2 Ac 23.3/1.9
2 90%aDGlcpN3N 97.3/5.03 52.8/4.06 53.3/4.13 68.9/3.58 74.0/3.86 61.7/3.82-3.86
3 Ac 23.0/1.98
bDGalf 107.3/5.12 87.5/4.20 76.7/4.28 83.7/4.02 70.4/4.01 70.4/3.64-3.90
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,2 | Ac |
| 1.9 | |
| 2 | 90%aDGlcpN3N | 5.03 | 4.06 | 4.13 | 3.58 | 3.86 | 3.82
3.86 |
| 3 | Ac |
| 1.98 | |
| | bDGalf | 5.12 | 4.20 | 4.28 | 4.02 | 4.01 | 3.64
3.90 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,2 | Ac | 176.1 | 23.3 | |
| 2 | 90%aDGlcpN3N | 97.3 | 52.8 | 53.3 | 68.9 | 74.0 | 61.7 |
| 3 | Ac | 175.4 | 23.0 | |
| | bDGalf | 107.3 | 87.5 | 76.7 | 83.7 | 70.4 | 70.4 |
|
There is only one chemically distinct structure:
Found 
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