Acteoside (verbascoside) was isolated as an analgesic principle from Cedron (leaves and stem of Lippia triphylla (L'HER) O. KUNTZE; Verbenaceae), a Peruvian medicinal plant, by activity-guided separation. The compound exhibited analgesia on acetic acid-induced writhing and on tail pressure pain in mice by the oral administration of 300 mg/kg and 100 mg/kg, respectively. Acteoside also caused weak sedation by its effect on the prolongation of pentobarbital-induced anesthesia and on the depression of locomotion enhanced by methamphetamine. An intravenous injection of acteoside reduced the effective dose to 2 mg/kg by the writhing method. Thirteen related compounds were tested for the activity by intravenous and oral administration to obtain information on the active structure.
phenylethanoid glycoside, acteoside, Lippia triphylla, analgesia, sedation
Publication DOI: 10.1248/cpb.45.499Journal NLM ID: 0377775Publisher: Pharmaceutical Society Of Japan
Institutions: Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba 263, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, IR, FAB-MS, TLC, chemical methods, UV, extraction, pharmacological assay, RP
Found