We successfully established hairy root cultures of Scutellaria baicalensis by direct inoculation on sterile seedlings with Agrobacterium rhizogenes pRil5834 harbouring pBI121. Transformation was proved by direct detection of the inserted T-DNA by the polymerase chain reaction. To determine the optimal medium for growth of hairy roots, the effects of five basal media were investigated; growth was best in B5 liquid medium. A new flavone glucoside, 5,7,2′,6′-tetrahydroxyflavone 2'-O-b-D-glucopyranoside 15 known flavonoids and five known phenylethanoids were isolated from the hairy root cultures of S. baicalensis for the first time. Their structures were elucidated by various spectroscopic methods.
polymerase chain reaction, 5, 7, flavonoids, Labiatae, Scutellaria baicalensis, hairy roots, Agrobacterium rhizogenes, 2', 6'-tetrahydroxyflavone 2'-D-b-D-glucopyranoside, phenylethanoids
Publication DOI: 10.1016/S0031-9422(96)00443-8Journal NLM ID: 0151434Publisher: Elsevier
Institutions: School of Pharmaceutical Sciences, Kitasato University, 9-1 Shirokane 5 Chome, Minato-ku, Tokyo 108, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, IR, PCR, FAB-MS, sugar analysis, chemical methods, HPLC, extraction
Found