Repetitive chromatography of the methanol extract of the roots of Gentiana tibetica afforded two new secoiridoid glycosides and a novel antifungal anthranilic acid derivative, together with beta-sitosterol, daucosterol, oleanolic acid, loganic acid, gentiopicroside, sweroside, 2'-(2,3-dihydroxybenzoyl)sweroside, trifloroside, rindoside and macrophylloside A. The structures of the new products were determined mainly by spectroscopic methods as 8-hydroxy-10-hydrosweroside, isomacrophylloside and ethyl N-docosanoylanthranilate. Ethyl N-docosanoylanthranilate inhibited the growth of the human pathogenic fungi Candida albicans and Aspergillus flavus. The taxonomic significance of the constituent is discussed briefly.
glycosides, fungicide, triterpene, sterols, iridoid, Gentianaceae, secoiridoids, Gentiana tibetica, 8-hydroxy-10-hydrosweroside, isomacrophylloside, ethyl N-docosanoylanthranilate
NCBI PubMed ID: 9611826Publication DOI: 10.1016/s0031-9422(97)00698-5Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Institute of Biotechnology, Department of Biological Science & Technology, Nanjing University, Nanjing, China
Methods: 13C NMR, 1H NMR, IR, FAB-MS, UV, extraction, optical rotation measurement, CI-MS, CC, COSY, HETCOR, COLOC
Found