The ethyl acetate soluble portion of the methanol extract of Mentha spicata var. crispa showed inhibitory activity on exocytosis in antigen- stimulated rat basophils. The bioassay-guided separation of the components of this fraction afforded 13 compounds. The chemical structures were elucidated to be four known flavonoids, three new and five known glucosides of lower alcohols and rosmarinic acid by means of chemical and spectroscopic evidences. Among them, three compounds showed biological activity. From the non-active 1-butanol soluble portion of the methanol extract, four new and two known structurally related glycosides were isolated and characterized.
flavonoids, Labiatae, aliphatic glucosides, antiallergic, exocytosis inhibitor, Mentha spicata
Publication DOI: 10.1016/S0031-9422(97)01112-6Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Hiroshima, Japan
Methods: 13C NMR, 1H NMR, FAB-MS, enzymatic hydrolysis, HPLC, extraction, optical rotation measurement, CD, CC, HR-FAB-MS
Found