The MeOH extract of the aerial parts of Croton ruizianus afforded two new pregnane glycosides 1 and 2, together with the morphinandienone alkaloids flavinantine (3) and O-methylflavinantine (4). Their structures were elucidated by NMR experiments including 1H-1H (1D TOCSY and 2D DQF-COSY) and 1H-13C (HSQC, HMBC) spectroscopy. The proaggregating activity of the MeOH extract and the isolates were evaluated. Although the MeOH extract and pregnane glycosides (at different doses) were found to promote platelet aggregation, flavinantine (3) and O-methylflavinantine (4) showed only slight activity. The ability of the MeOH extract and the four compounds to act synergistically with thrombin was also evaluated. All the tested compounds were successful in augmenting the aggregating effect of thrombin, although to different degrees.
Magnetic Resonance Spectroscopy, glycosides, blood platelets, cell survival, medicinal plants, Croton ruizianus
NCBI PubMed ID: 9544561Publication DOI: 10.1021/np970399dJournal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Institutions: Dipartimento di Scienze Farmaceutiche, Penta di Fisciano Salerno, Italy, Dipartimento Biochimica e Biotecnologie Mediche, Napoli, Italy, Facultad de Farmacia y Bioquimica, Universidad Inca Garcilaso de la Vega, Lima, Peru
Methods: 13C NMR, 1H NMR, FAB-MS, biological assays, extraction, optical rotation measurement, TOCSY, cytotoxicity assay, DQF-COSY, HMBC, DEPT
Found