Taxonomic group: bacteria / Proteobacteria, Firmicutes
(Phylum: Proteobacteria, Firmicutes)
Associated disease: infection due to Helicobacter pylori [ICD11:
XN3DY 
]
NCBI PubMed ID: 23990317Publication DOI: 10.1007/s10719-013-9496-4Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Eleonora.altman
nrc-cnrc.gc.ca
Institutions: National Research Council Canada, Ottawa, Ontario, K1A 0R6, Canada
We have recently demonstrated that synthetic glycoconjugates based on delipidated lipopolysaccharide (LPS) of Helicobacter pylori and containing an α(1-6)-glucan chain induced broadly cross-reactive functional antibodies in immunized animals. To investigate the candidacy of α(1-6)-glucan as an alternative vaccine strategy we prepared glycoconjugates based on dextrans produced by lactic acid bacteria Leuconostoc mesenteroides B512F and consisting of linear α(1-6)-glucan chains with limited branching. Three dextrans with averaged molecular masses of 5,000 Da, 3,500 Da and 1,500 Da, respectively, were modified with a diamino group-containing linker and conjugated to a carrier protein, tetanus toxoid (TT) or diphtheria toxoid (DT), and their immunological properties investigated. The conjugates were immunogenic in both rabbits and mice and induced specific IgG responses against α(1-6)-glucan-expressing H. pylori LPS. Studies performed with post-immune sera of mice and rabbits immunized with dextran-based conjugates demonstrated cross-reactivity with LPS from typeable and non-typeable strains of H. pylori and selected mutants. The post-immune sera from rabbits that received the conjugates exhibited functional activity against α(1-6)-glucan-positive strains of H. pylori. These data provide evidence that dextran-based conjugates may offer a simplified approach to the development of carbohydrate-based vaccines against H. pylori.
Helicobacter pylori, Helicobacter, conjugate vaccine, glycoconjugate, Dextran, Bactericidal assay
Structure type: homopolymer
Location inside paper: p.13
Trivial name: glucan, α-D-glucan, dextran, α-1,6-glucan, α-(1,6)-glucan, dextran, α-(1,6)-glucan, α-1,6 dextran, β-1,3-D-glucan, polysaccharide IOP
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: 1H NMR, methylation, MALDI-TOF MS, biological assays, microscopy, conjugation, MALDI-TOF/TOF MS, bactericidal activity
Biological activity: testing immunogenicity of synthetic glycoconjugates
Related record ID(s): 30205
NCBI Taxonomy refs (TaxIDs): 210,
1245Reference(s) to other database(s): GTC:G69605LY, GlycomeDB:
2461
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Helicobacter pylori [ICD11:
XN3DY ]
NCBI PubMed ID: 23990317Publication DOI: 10.1007/s10719-013-9496-4Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Eleonora.altman
nrc-cnrc.gc.ca
Institutions: National Research Council Canada, Ottawa, Ontario, K1A 0R6, Canada
We have recently demonstrated that synthetic glycoconjugates based on delipidated lipopolysaccharide (LPS) of Helicobacter pylori and containing an α(1-6)-glucan chain induced broadly cross-reactive functional antibodies in immunized animals. To investigate the candidacy of α(1-6)-glucan as an alternative vaccine strategy we prepared glycoconjugates based on dextrans produced by lactic acid bacteria Leuconostoc mesenteroides B512F and consisting of linear α(1-6)-glucan chains with limited branching. Three dextrans with averaged molecular masses of 5,000 Da, 3,500 Da and 1,500 Da, respectively, were modified with a diamino group-containing linker and conjugated to a carrier protein, tetanus toxoid (TT) or diphtheria toxoid (DT), and their immunological properties investigated. The conjugates were immunogenic in both rabbits and mice and induced specific IgG responses against α(1-6)-glucan-expressing H. pylori LPS. Studies performed with post-immune sera of mice and rabbits immunized with dextran-based conjugates demonstrated cross-reactivity with LPS from typeable and non-typeable strains of H. pylori and selected mutants. The post-immune sera from rabbits that received the conjugates exhibited functional activity against α(1-6)-glucan-positive strains of H. pylori. These data provide evidence that dextran-based conjugates may offer a simplified approach to the development of carbohydrate-based vaccines against H. pylori.
Helicobacter pylori, Helicobacter, conjugate vaccine, glycoconjugate, Dextran, Bactericidal assay
Structure type: oligomer
Location inside paper: p.23
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_136044,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_2189046,IEDB_2189047,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 1H NMR, methylation, MALDI-TOF MS, biological assays, microscopy, conjugation, MALDI-TOF/TOF MS, bactericidal activity
Biological activity: testing immunogenicity of synthetic glycoconjugates
Comments, role: a linear backbone of the LPS of the mutant strain H. pylori 26695 HP0826::Kan
Related record ID(s): 30038
NCBI Taxonomy refs (TaxIDs): 85962Reference(s) to other database(s): GTC:G20238HE
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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