The main triterpene glycosides of Cimicifuga racemosa were separated by reversed phase HPLC, using a C-18 column, Evaporative Light Scattering (ELS) detection and a grient system consisting of water, acetonitrile and reagent alcohol. Within 35 min three main glycosides could be separated and quantified in the methanolic root extract with detection limits of 10.5, 15.6 and 31.6 μg/mL respectively. The method was successfully used, to analyzed different Cimicifuga racemosa market products, as well as to distinguish between other Cimicifuga samples from China.
triterpene glycosides, column liquid chromatography, evaporative light scattering detection, Cimicifuga racemosa, black cohosh
Publication DOI: 10.1007/BF02491021Journal NLM ID: 0316520Publisher: Friedr. Vieweg Und Sohn Verlags Gmbh
Institutions: Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, USA, Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, US
Methods: HPLC, extraction, LC-MS, sonication, centrifugation
Found