Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 33035917Publication DOI: 10.1016/j.carres.2020.108163Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M. Urai <mu206177
nodai.ac.jp>
Institutions: Department of Bioscience in Daily Life, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510, Japan, Department of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture, Tokyo, 156-8502, Japan
Acid sulfate soil is found throughout Southeast Asia, and its strong acidity (pH 2-4) is accompanied by various plant growth-inhibiting factors that can reduce crop production. Among these factors, aluminum elution from the soil due to soil acidity strongly inhibits crop growth and is particularly problematic for agricultural production. We previously isolated Acidocella aluminiidurans strain AL46, a highly aluminum-tolerant bacterium, from the rhizospheres of the grass Panicum repens, inhabiting the acid sulfate soil in Vietnam. To elucidate the mechanism underlying the high aluminum tolerance of strain AL46, in the present study, we investigated the aluminum-adsorption ability of strain AL46 surface polysaccharides and confirmed the strong adsorption ability of the capsular polysaccharide (AL46CPS). Based on this finding, we further determined the chemical structure of AL46CPS using 1H and 13C NMR spectroscopy by conducting 2D DQF-COSY, TOCSY, HSQC, HMBC, and NOESY experiments. AL46CPS comprises a trisaccharide repeating unit with the following structure: [→2)-β-d-Rhap-(1→3)-α-d-Rhap-(1→2)-α-d-Rhap-(1→]n. These findings highlight the potential application of AL46CPS as a new aluminum-adsorbing substance in acidic environments to prevent crop loss.
structure, NMR spectroscopy, Acidocella aluminiidurans, Actual acid sulfate soil, Aluminum-binding capsular polysaccharide, Aluminum-tolerant bacterium
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, AL46CPS
Compound class: CPS
Contained glycoepitopes: IEDB_1394181
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GLC-MS, composition analysis, measuring the Al3+-adsorbing ability of polysaccharides
NCBI Taxonomy refs (TaxIDs): 472056Reference(s) to other database(s): GTC:G08270CB
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3 bDRhap 97.6 78.4 74.2 70.0 73.4 17.6
2 aDRhap 102.5 68.2 78.1 71.3 69.9 17.5
aDRhap 100.9 78.9 70.7 73.1 69.6 17.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3 bDRhap 4.70 3.97 3.59 3.32 3.31 1.22
2 aDRhap 4.92 4.11 3.83 3.43 3.62 1.17
aDRhap 5.10 4.00 3.90 3.33 4.03 1.18
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3 bDRhap 97.6/4.70 78.4/3.97 74.2/3.59 70.0/3.32 73.4/3.31 17.6/1.22
2 aDRhap 102.5/4.92 68.2/4.11 78.1/3.83 71.3/3.43 69.9/3.62 17.5/1.17
aDRhap 100.9/5.10 78.9/4.00 70.7/3.90 73.1/3.33 69.6/4.03 17.5/1.18
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3 | bDRhap | 4.70 | 3.97 | 3.59 | 3.32 | 3.31 | 1.22 |
| 2 | aDRhap | 4.92 | 4.11 | 3.83 | 3.43 | 3.62 | 1.17 |
| | aDRhap | 5.10 | 4.00 | 3.90 | 3.33 | 4.03 | 1.18 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3 | bDRhap | 97.6 | 78.4 | 74.2 | 70.0 | 73.4 | 17.6 |
| 2 | aDRhap | 102.5 | 68.2 | 78.1 | 71.3 | 69.9 | 17.5 |
| | aDRhap | 100.9 | 78.9 | 70.7 | 73.1 | 69.6 | 17.5 |
|
There is only one chemically distinct structure:
Found 
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