Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperPublication DOI: 10.1007/s00792-019-01113-8Journal NLM ID: 9706854Publisher: Springer
Correspondence: Angela Casillo <angela.casillo
unina.it>; Maria Michela Corsaro <corsaro
unina.it>
Institutions: Department of Chemical Sciences, UniversitŠ° degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia, 80126, Naples, Italy
In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid.
capsular polysaccharide, pseudaminic acid, Psychrobacter, Psychrotolerant, Extracellular polysaccharide (EPS)
Structure type: polymer chemical repeating unit
Location inside paper: p.67, scheme 1, table 1
Compound class: CPS
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_838988,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, DOC-PAGE, dialysis, thermal hysteresis, IRI
NCBI Taxonomy refs (TaxIDs): 259536Reference(s) to other database(s): GTC:G36027NE
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8 C9
4 aDGlcp 97.5 72.1 70.8 73.8 73.2 61.7
5 Ac 173.6-174.2 22.9-23.1
7 Ac
8 bDGlcp 103.4 74.2 76.6 70.8 77.0 61.9
bXPsep 172.6 101.8 33.8 72.0 47.1 72.4 53.6 78.5 16.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8 H9
4 aDGlcp 5.05 3.52 3.37 3.59 3.85 3.76-3.85
5 Ac - 1.97-2.04
7 Ac - 1.97-2.04
8 bDGlcp 4.58 3.20 3.52 3.36 3.49 3.74-3.90
bXPsep - - 1.68-2.62 3.92 4.38 4.02 4.28 4.43 1.29
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8 C9/H9
4 aDGlcp 97.5/5.05 72.1/3.52 70.8/3.37 73.8/3.59 73.2/3.85 61.7/3.76-3.85
5 Ac 22.9-23.1/1.97-2.04
7 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
8 bDGlcp 103.4/4.58 74.2/3.20 76.6/3.52 70.8/3.36 77.0/3.49 61.9/3.74-3.90
bXPsep 33.8/1.68-2.62 72.0/3.92 47.1/4.38 72.4/4.02 53.6/4.28 78.5/4.43 16.0/1.29
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |
|---|
| 4 | aDGlcp | 5.05 | 3.52 | 3.37 | 3.59 | 3.85 | 3.76
3.85 | |
| 5 | Ac |
| 1.97
2.04 | |
| 7 | Ac |
| 1.97
2.04 | |
| 8 | bDGlcp | 4.58 | 3.20 | 3.52 | 3.36 | 3.49 | 3.74
3.90 | |
| | bXPsep |
|
| 1.68
2.62 | 3.92 | 4.38 | 4.02 | 4.28 | 4.43 | 1.29 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
|---|
| 4 | aDGlcp | 97.5 | 72.1 | 70.8 | 73.8 | 73.2 | 61.7 | |
| 5 | Ac | 173.6
174.2 | 22.9
23.1 | |
| 7 | Ac | |
| 8 | bDGlcp | 103.4 | 74.2 | 76.6 | 70.8 | 77.0 | 61.9 | |
| | bXPsep | 172.6 | 101.8 | 33.8 | 72.0 | 47.1 | 72.4 | 53.6 | 78.5 | 16.0 |
|
There is only one chemically distinct structure:
Found 
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