In vivo bioassay-guided fractionation of the aqueous alcohol extract of the aerial parts of Bidens pilosa Sch. Bip. var. radiata (Asteraceae) using C57BL/Ks-db/db mice as a model for type 2 diabetes, yielded two known polyacetylenic glucosides, identified as 2-β-D-glucopyranosyloxy-1-hydroxy-5(E)-tridecene-7,9,11-triyne (1) and 3-β-D-glucopyranosyloxy-1-hydroxy-6(E)-tetradecene-8,10,12-triyne (2). A 3:2 mixture of compounds 1 and 2 effected a significant drop in blood glucose.
Bidens pilosa, acetylenic glucosides, antihyperglycemic activity
NCBI PubMed ID: 10705745Publication DOI: 10.1055/s-0029-1243117Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: dfort@shaman.com
Institutions: Shaman Pharmaceuticals Inc., South San Francisco, USA, Phytera, Inc., Worcester, USA
Methods: 13C NMR, 1H NMR, NMR-2D, FAB-MS, TLC, ESI-MS, biological assays, HPLC, extraction, CC
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