Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: spore
The structure was elucidated in this paperJournal NLM ID: 0043535WWW link: http://www.sciencedirect.com/science/article/pii/S0008621501002385Publisher: Elsevier
Correspondence: jnfang
mail.shcnc.ac.cn
Institutions: Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Tai-Yuan Road, 200031, P.R., Shanghai, China
A linear (1→3)-α-d-glucan was isolated from the spores of Ganoderma lucidum (Fr.) Karst. Six different functionalized derivatives of the (1→3)-α-d-glucan—aminopropylated, hydroxyethylated, sulfated, carboxymethylated, carboxymethylated and sulfated, and benzylamidated–carboxymethylated—with varying degrees of substitution were synthesized. The structural features and physicochemical properties of all derivatives were investigated by means of chemical and spectral analyses, and their effects on lymphocyte proliferation and antibody production were tested in vitro and in vivo. In general, the structural and physicochemical properties, and lymphocyte proliferation activity of all samples varied with the functionalized groups and the degree of substitution. The results of immunological assays indicated that some modified derivatives had potent stimulating effects on lymphocyte proliferation and antibody production and the introduction of carboxymethyl group with low degree of substitution (DS<0.28) was the best choice on the improvement of the immunostimulating activity.
chemical modification, immunological activity, (1→3)-α-D-glucan, Ganoderma lucidum
Structure type: homopolymer
Location inside paper: p. 128
Trivial name: D-rhamnan, α-1,3-D-glucan, α-1,3-glucan, (1-3)-α-Glucan, 1-3-α-glucan, α-(1,3)-glucan, α-(1,3)glucan, (1-3)-α-glucan, pseudonigeran, α-(1,3)-glucan, pseudonigeran, water-insoluble α-D-glucan (TM-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, D-glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GLC-MS, GLC, UV, sulfation, methylation analysis, carboxymethylation, FTIR spectroscopy, aminopropylation, hydroxyethylation, immunological assay
Comments, role: data on immunological activity of chemical modifications are present
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G02177KX, CCSD:
49417, CBank-STR:17683, GenDB:HM245773
Show glycosyltransferases
NMR conditions: in D2O / 2%NaOD
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 102.6 75.1 84.7 72.9 73.4 63.5
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 102.6 | 75.1 | 84.7 | 72.9 | 73.4 | 63.5 |
|
There is only one chemically distinct structure:
Found 
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