Three new compounds, (24R,S)-3α-acetoxy-24-hydroxy-5α-lanosta-8,25-dien-21-oic acid, named tsugaric acid C (1); 3α-acetoxy-5α-lanosta-8,24-diene-21-O-β-D-xyloside, named tsugarioside B (2); and 3α-acetoxy-(Z)-24-methyl-5α-lanosta-8,23,25-trien-21-oic acid ester β-D-xyloside, named tsugarioside C (3), and a mixture of two known steroids were isolated from the fruit bodies of Ganoderma tsugae. The structures of 1−3 were determined by spectral and chemical methods. The cytotoxic activity of the lanostanoid constituents of this fungus was evaluated against several different cancer cell lines.

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6	C7	C8	C9	C10	C11	C12	C13	C14	C15	C16	C17	C18	C19	C20	C21	C22	C23
21	bDXylp	102.7	71.9	73.7	69.7	63.7
3	Ac	170.9	21.4
	Subst	30.2	23.3	78.1	36.7	45.3	18.0	26.0	134.0	134.6	36.9	21.0	30.8	44.3	49.9	27.5	29.7	40.6	16.1	19.0	44.9	70.2	30.7	24.7


1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

CC(=O)O[C@@H]1CC[C@]2(C)C3=C(CC[C@H]2C1(C)C)[C@]1(C)CC[C@H]([C@@H](CCC=C(C)C)CO[C@@H]2OC[C@@H](O)[C@H](O)[C@H]2O)[C@@]1(C)CC3

616.88 g/mol (C₃₇H₆₀O₇)

Three new compounds, (24R,S)-3α-acetoxy-24-hydroxy-5α-lanosta-8,25-dien-21-oic acid, named tsugaric acid C (1); 3α-acetoxy-5α-lanosta-8,24-diene-21-O-β-D-xyloside, named tsugarioside B (2); and 3α-acetoxy-(Z)-24-methyl-5α-lanosta-8,23,25-trien-21-oic acid ester β-D-xyloside, named tsugarioside C (3), and a mixture of two known steroids were isolated from the fruit bodies of Ganoderma tsugae. The structures of 1−3 were determined by spectral and chemical methods. The cytotoxic activity of the lanostanoid constituents of this fungus was evaluated against several different cancer cell lines.

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6	C7	C8	C9	C10	C11	C12	C13	C14	C15	C16	C17	C18	C19	C20	C21	C22	C23
21	bDXylp	94.5	72.3	75.9	69.5	65.8
3	Ac	170.9	21.4
	Subst	30.4	23.3	78.1	36.7	45.3	18.0	25.9	133.7	134.7	36.9	20.8	30.8	44.3	49.5	27.0	28.9	47.0	16.3	19.0	47.8	175.2	32.9	123.5


1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C=C(C)/C(C)=C/C[C@@H](CO[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O)[C@H]1CC[C@@]2(C)C3=C(CC[C@]12C)[C@@]1(C)CC[C@@H](OC(C)=O)C(C)(C)[C@@H]1CC3

628.891 g/mol (C₃₈H₆₀O₇)

Three new compounds, (24R,S)-3α-acetoxy-24-hydroxy-5α-lanosta-8,25-dien-21-oic acid, named tsugaric acid C (1); 3α-acetoxy-5α-lanosta-8,24-diene-21-O-β-D-xyloside, named tsugarioside B (2); and 3α-acetoxy-(Z)-24-methyl-5α-lanosta-8,23,25-trien-21-oic acid ester β-D-xyloside, named tsugarioside C (3), and a mixture of two known steroids were isolated from the fruit bodies of Ganoderma tsugae. The structures of 1−3 were determined by spectral and chemical methods. The cytotoxic activity of the lanostanoid constituents of this fungus was evaluated against several different cancer cell lines.

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

CC(=O)O[C@@H]1CC[C@]2(C)C3=C(CC[C@H]2C1(C)C)[C@]1(C)CC[C@H]([C@@H](CCC=C(C)C)C(=O)O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@]1(C)CC3

660.889 g/mol (C₃₈H₆₀O₉)