Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: caterpillar part
NCBI PubMed ID: 34364580Publication DOI: 10.1016/j.carbpol.2021.118443Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: A. Lyu <aipinglu
hkbu.edu.hk>; S. Han <simonhan
hkbu.edu.hk>
Institutions: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
Cordyceps, one of the most expensive natural health supplements, is popularly used to modulate immune function. However, little is known regarding the underlying mechanism of its immunomodulatory activity. We newly reported a Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-α glucan by chemical and spectral analysis and is able to induce significant immune responses of macrophages. Herein, we further investigated the molecular mechanism of CCP's immunomodulatory effects. The results indicate that CCP modulates the TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role as verified on TLR4-deficient (TLR4-/-) bone marrow-derived macrophages (BMDMs) and TLR4-/- mice. These findings provide a precise understanding of the molecular mechanism of Cordyceps' immunomodulatory benefits.
polysaccharide, macrophage, immune responses, Cordyceps, TLR4/MyD88/p38
Structure type: fragment of a bigger structure ; 433778
Location inside paper: p. 118443-2
Trivial name: Cordyceps polysaccharide marker CCP
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420419,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: NMR-2D, methylation, GC-MS, sugar analysis, ELISA, immunoblotting, statistical analysis, cytokine assay, cell viability assay, flow cytometry, HPGPC, immunomodulatory activity analysis, NO production, animal studies
Biological activity: polysaccharide marker CCP could significantly induce IL-6 and TNF-α production of macrophages through the direct and selective TLR4/MyD88/p38 axis
NCBI Taxonomy refs (TaxIDs): 72228
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There is only one chemically distinct structure:
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(later renamed to: Ophiocordyceps sinensis)