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Kumar M, Singh A, Kumari S, Kumar P, Wasi M, Mondal AK, Rudramurthy SM, Chakrabarti A, Gaur NA, Gow NAR, Prasad R
Sphingolipidomics of drug resistant Candida auris clinical isolates reveal distinct sphingolipid species signatures
Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids 1866(1) (2021)
ID 158815
Candida auris
(NCBI TaxID 498019,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32942047Publication DOI: 10.1016/j.bbalip.2020.158815Journal NLM ID: 0217513Publisher: Elsevier
Correspondence: Gaur NA <naseem
icgeb.res.in>; Prasad R <rprasad
ggn.amity.edu>
Institutions: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, Amity Institute of Integrative Science and Health, and Amity Institute of Biotechnology, Amity University Gurgaon, Haryana, India, Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, Department of Biochemistry, University of Lucknow, Lucknow, India, The Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh, India, Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, UK
Independent studies from our group and others have provided evidence that sphingolipids (SLs) influence the antimycotic susceptibility of Candida species. We analyzed the molecular SL signatures of drug-resistant clinical isolates of Candida auris, which have emerged as a global threat over the last decade. This included Indian hospital isolates of C. auris, which were either resistant to fluconazole (FLCR) or amphotericin B (AmBR) or both drugs. Relative to Candida glabrata and Candida albicans strains, these C. auris isolates were susceptible to SL pathway inhibitors such as myriocin and aureobasidin A, suggesting that SL content may influence azole and AmB susceptibilities. Our analysis of SLs confirmed the presence of 140 SL species within nine major SL classes, namely the sphingoid bases, Cer, αOH-Cer, dhCer, PCer, αOH-PCer, αOH-GlcCer, GlcCer, and IPC. Other than for αOH-GlcCer, most of the SLs were found at higher concentrations in FLCR isolates as compared to the AmBR isolates. SLs were at intermediate levels in FLCR + AmBR isolates. The observed diversity of molecular species of SL classes based on fatty acyl composition was further reflected in their distinct specific imprint, suggesting their influence in drug resistance. Together, the presented data improves our understanding of the dynamics of SL structures, their synthesis, and link to the drug resistance in C. auris.
sphingolipids, glucosylceramides, LC-MS/MS, multiple drug resistance, phytoceramide
Structure type: monomer
Location inside paper: Fig. 1
Compound class: glycolipid
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: alkaline hydrolysis, extraction, cell growth, centrifugation, optical density measurement, QTRAP-MS
Related record ID(s): 40993, 40994
NCBI Taxonomy refs (TaxIDs): 498019
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There is only one chemically distinct structure:
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Kumar M, Singh A, Kumari S, Kumar P, Wasi M, Mondal AK, Rudramurthy SM, Chakrabarti A, Gaur NA, Gow NAR, Prasad R
Sphingolipidomics of drug resistant Candida auris clinical isolates reveal distinct sphingolipid species signatures
Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids 1866(1) (2021)
ID 158815
Candida auris
(NCBI TaxID 498019,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32942047Publication DOI: 10.1016/j.bbalip.2020.158815Journal NLM ID: 0217513Publisher: Elsevier
Correspondence: Gaur NA <naseem
icgeb.res.in>; Prasad R <rprasad
ggn.amity.edu>
Institutions: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, Amity Institute of Integrative Science and Health, and Amity Institute of Biotechnology, Amity University Gurgaon, Haryana, India, Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, Department of Biochemistry, University of Lucknow, Lucknow, India, The Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh, India, Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, UK
Independent studies from our group and others have provided evidence that sphingolipids (SLs) influence the antimycotic susceptibility of Candida species. We analyzed the molecular SL signatures of drug-resistant clinical isolates of Candida auris, which have emerged as a global threat over the last decade. This included Indian hospital isolates of C. auris, which were either resistant to fluconazole (FLCR) or amphotericin B (AmBR) or both drugs. Relative to Candida glabrata and Candida albicans strains, these C. auris isolates were susceptible to SL pathway inhibitors such as myriocin and aureobasidin A, suggesting that SL content may influence azole and AmB susceptibilities. Our analysis of SLs confirmed the presence of 140 SL species within nine major SL classes, namely the sphingoid bases, Cer, αOH-Cer, dhCer, PCer, αOH-PCer, αOH-GlcCer, GlcCer, and IPC. Other than for αOH-GlcCer, most of the SLs were found at higher concentrations in FLCR isolates as compared to the AmBR isolates. SLs were at intermediate levels in FLCR + AmBR isolates. The observed diversity of molecular species of SL classes based on fatty acyl composition was further reflected in their distinct specific imprint, suggesting their influence in drug resistance. Together, the presented data improves our understanding of the dynamics of SL structures, their synthesis, and link to the drug resistance in C. auris.
sphingolipids, glucosylceramides, LC-MS/MS, multiple drug resistance, phytoceramide
Structure type: monomer
Location inside paper: Fig. 1
Compound class: glycolipid
Contained glycoepitopes: IEDB_130701,IEDB_144983,IEDB_144993,IEDB_152206,IEDB_983930,SB_44,SB_67,SB_72
Methods: alkaline hydrolysis, extraction, cell growth, centrifugation, optical density measurement, QTRAP-MS
Related record ID(s): 40992, 40994
NCBI Taxonomy refs (TaxIDs): 498019
Show glycosyltransferases
There is only one chemically distinct structure:
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Kumar M, Singh A, Kumari S, Kumar P, Wasi M, Mondal AK, Rudramurthy SM, Chakrabarti A, Gaur NA, Gow NAR, Prasad R
Sphingolipidomics of drug resistant Candida auris clinical isolates reveal distinct sphingolipid species signatures
Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids 1866(1) (2021)
ID 158815
|
LIP-(1-2)-+
|
myoIno-(1--P--6)--a-D-Manp-(1-2)-myoIno-(1--P--1)--SPH |
Show graphically |
Candida auris
(NCBI TaxID 498019,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32942047Publication DOI: 10.1016/j.bbalip.2020.158815Journal NLM ID: 0217513Publisher: Elsevier
Correspondence: Gaur NA <naseem
icgeb.res.in>; Prasad R <rprasad
ggn.amity.edu>
Institutions: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, Amity Institute of Integrative Science and Health, and Amity Institute of Biotechnology, Amity University Gurgaon, Haryana, India, Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, Department of Biochemistry, University of Lucknow, Lucknow, India, The Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh, India, Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, UK
Independent studies from our group and others have provided evidence that sphingolipids (SLs) influence the antimycotic susceptibility of Candida species. We analyzed the molecular SL signatures of drug-resistant clinical isolates of Candida auris, which have emerged as a global threat over the last decade. This included Indian hospital isolates of C. auris, which were either resistant to fluconazole (FLCR) or amphotericin B (AmBR) or both drugs. Relative to Candida glabrata and Candida albicans strains, these C. auris isolates were susceptible to SL pathway inhibitors such as myriocin and aureobasidin A, suggesting that SL content may influence azole and AmB susceptibilities. Our analysis of SLs confirmed the presence of 140 SL species within nine major SL classes, namely the sphingoid bases, Cer, αOH-Cer, dhCer, PCer, αOH-PCer, αOH-GlcCer, GlcCer, and IPC. Other than for αOH-GlcCer, most of the SLs were found at higher concentrations in FLCR isolates as compared to the AmBR isolates. SLs were at intermediate levels in FLCR + AmBR isolates. The observed diversity of molecular species of SL classes based on fatty acyl composition was further reflected in their distinct specific imprint, suggesting their influence in drug resistance. Together, the presented data improves our understanding of the dynamics of SL structures, their synthesis, and link to the drug resistance in C. auris.
sphingolipids, glucosylceramides, LC-MS/MS, multiple drug resistance, phytoceramide
Structure type: monomer
Location inside paper: Fig. 1
Compound class: glycolipid
Contained glycoepitopes: IEDB_130701,IEDB_144983,IEDB_144993,IEDB_152206,IEDB_474450,IEDB_983930,SB_44,SB_67,SB_72
Methods: alkaline hydrolysis, extraction, cell growth, centrifugation, optical density measurement, QTRAP-MS
Related record ID(s): 40992, 40993
NCBI Taxonomy refs (TaxIDs): 498019
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
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