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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 34606556
Publication DOI: 10.1039/d1fo01683k
Journal NLM ID: 101549033
Publisher: Cambridge: Royal Society of Chemistry
Correspondence: Yang L <yangliu199

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Institutions: School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea, Engineering Research Center of Bio-process, School of Food and Biological Engineering, Hefei University of Technology, Hefei, China, Department of Respiratory Medicine, Hunan Children's Hospital, Hunan, China

The extracellular polysaccharide of Morchella esculenta cultivated under submerged fermentation was extracted. A single polysaccharide was purified through DEAE-Cellulose 52 and Sephadex G 100, and named as MEP 2a. The molecular weight of MEP 2a was determined by HPGPC and it is about 1391.5 kDa. MEP 2a is composed of mannose and glucose as the monosaccharide unit with a molar ratio of 8.15 : 1.07. The main polysaccharide chemical structure was analyzed by 1D and 2D NMR. Methylation and NMR analysis revealed that the backbone of MEP 2a consists of 1,3,4-linked-Manp, 1,2-linked-Manp and 1,6-linked-Glcp. 1D and 2D NMR results indicated that the main chain is based on →1)-β-D-Glcp-(6→, →1)-α-D-Manp-(3,4→, →1)-α-D-Manp-(2→) and the branch chain is composed of α-D-Manp-(1→, →1)-β-D-Glcp-(6→ and α-D-Glcp-(1→). MEP 2a promoted the phagocytosis function and secretion of NO, IL-1β, IL-6 and TNF-α of macrophages. In the present study, the chemical structure and immunomodulatory ability of an extracellular polysaccharide of Morchella esculenta was investigated which guarantees further research studies and promising applications.

NMR, structure, polysaccharide, fermentation, immunomodulatory activity, Morchella esculenta

Structure type: structural motif or average structure ; 1391500
Location inside paper: p. 9332, Fig. 3, f, p. 9333, Table 2
Compound class: polysaccharide, glucomannan
Contained glycoepitopes: IEDB_130701,IEDB_140628,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_164174,IEDB_983930,IEDB_983931,SB_192,SB_197,SB_44,SB_67,SB_72

Methods: 13C NMR, 1H NMR, NMR-2D, methylation, IR, ELISA, acid hydrolysis, anion-exchange chromatography, GC, Western blotting, GPC, UV, extraction, acetylation, reduction, cell growth, dialysis, determination of NO production, cytokine production, cell viability assay, HPGPC, SEM, complex formation with Congo Red, phagocytosis assay, AFM, Sevag methodf
Comments, role: the structure is heavily reworked, the original structure is not possible

NCBI Taxonomy refs (TaxIDs): 39407
Show glycosyltransferases

NMR conditions: in D2O at 298 K

¹H NMR data: present in publication

¹³C NMR data: present in publication

There is only one chemically distinct structure:

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SVG MW SMILES 3D mol Ionize