An acid polysaccharide, named HP, was produced by endophytic Penicillium javanicum MSC-R1 isolated from southern medicine Millettia speciosa Champ. The molecular weight of HP was 37.8 kDa and consisted of Ara f, Galр, Glcр, Manр, and GlcрA with a molar ratio of 1.09: 3.47: 68.48: 16.59: 8.85. The glycosidic linkage of HP was proven to be →3,4)-α-D-Glcр-(1→6)-α-D-Manр-(1→, →3,4)-α-D-Glcр-(1→4)-α-D-Glcр-(1→, →3),→6)-α-D-Manр-(1→4)-α-D-Glcр-(1→, →3), β-D-Galр-(1→3)-α-D-Glcр-(1→, →4),→5)-α-L-Araf-(1→3)-α-D-Glcр-(1→, →4),→6)-α-D-Manр-(1→4)-α-D-GlcAр-(1→ and →4)-α-D-GlcAр-(1→4)-α-D-Glcр-(1→, →3). Additionally, 250 μg/mL of HP possessed nontoxicity to RAW 264.7 cells and exhibited anti-inflammation activity. HP could significantly restrain the amount of tumor necrosis factor-α, interleukin-6 and NO release in RAW264.7, which property is possibly associated with its abundant glucosidic linkage. These results indicated that HP could be regarded as a ponderable ingredient for the health-beneficial functional foods.

structural, polysaccharide, exopolysaccharide, molecular weight, biological activities, anti-inflammation, Penicillium

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,3	bDGalp	104.40	72.71	75.88	71.05	?	60.75
4,4,4,6,3	aLAraf	109.71	80.62	75.98	84.62	?
4,4,4,6,4	aDGlcp
4,4,4,6	aDGlcp
4,4,4	aDManp	101.45	71.44	76.79	69.22	69.60	73.34
4,4	aDGlcpA	102.33	71.54	73.35	75.80	71.22	162.62
4	aDGlcp
	aDGlcp


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,3	bDGalp	4.49	3.38	3.54	4.49	?	3.75
4,4,4,6,3	aLAraf	5.02	4.12	3.94	3.69	3.96
4,4,4,6,4	aDGlcp
4,4,4,6	aDGlcp	5.34	4.52	4.15	3.70	3.90	3.76
4,4,4	aDManp	5.23	3.77	3.79	3.87	3.66	3.56
4,4	aDGlcpA	4.35	3.29	3.56	3.73	4.55	-
4	aDGlcp	5.34	4.52	4.15	3.70	3.90	3.76
	aDGlcp


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,3	bDGalp	104.40/4.49	72.71/3.38	75.88/3.54	71.05/4.49	?/?	60.75/3.75
4,4,4,6,3	aLAraf	109.71/5.02	80.62/4.12	75.98/3.94	84.62/3.69	?/3.96
4,4,4,6,4	aDGlcp
4,4,4,6	aDGlcp	NMR TSV error 2: unequal length of 13C and 1H datasets
4,4,4	aDManp	101.45/5.23	71.44/3.77	76.79/3.79	69.22/3.87	69.60/3.66	73.34/3.56
4,4	aDGlcpA	102.33/4.35	71.54/3.29	73.35/3.56	75.80/3.73	71.22/4.55	
4	aDGlcp	NMR TSV error 2: unequal length of 13C and 1H datasets
	aDGlcp

The spectrum also has 2 signals at unknown positions (not plotted).

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

O=C(O)[C@H]1O[C@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O[C@H]1O[C@H](CO[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O[C@@H]3O[C@@H](CO)[C@H](O)[C@H]3O)[C@H]2O)[C@@H](O)[C@H](O)[C@@H]1O

1299.1 g/mol (C₄₇H₇₈O₄₁)