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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 37570759
Publication DOI: 10.3390/molecules28155788
Journal NLM ID: 100964009
Publisher: Basel, Switzerland: MDPI
Correspondence: M.H. Wang <mhwang

kangwon.ac.kr>
Institutions: Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Republic of Korea, Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of Korea

Polysaccharides are abundantly present in fungi and are gaining recognition for their exceptional bioactivities. Hence, the present study aimed to extract intracellular polysaccharides (IPS-1 and IPS-2) from the endophytic Penicillium radiatolobatum and compare their physicochemical and bioactive attributes. The monosaccharide composition analysis revealed the existence of galactose, glucose, and mannose in both the IPS, while a trace amount of xylose was found in IPS-1. Further, FT-IR, 1H NMR, and 13C NMR analysis suggested that the IPS-2 was mainly composed of the β-(1→4)-D-Galactose and β-(1→4)-D-Glucose as the main chain, with the β-(1→6)-D-mannose as branched chains. Compared to IPS-1, the IPS-2 showed higher antioxidant activities with an IC50 value of 108 ± 2.5 μg/mL, 272 ± 4.0 μg/mL, and 760 ± 5.0 μg/mL for ABTS+ scavenging, DPPH radical scavenging, and ferric reducing power, respectively. In addition, the IPS-2 inhibited the viability of prostate cancer (PC-3) cells (IC50; 435 ± 3.0 μg/mL) via apoptosis associated with mitochondrial membrane potential collapse and altered morphological features, which was revealed by cellular staining and flow cytometric analysis. Moreover, no apparent cytotoxic effects were seen in IPS-2-treated (1000 μg/mL) non-cancerous cells (HEK-293 and NIH3T3). Overall, the findings of this study suggest that P. radiatolobatum could be a potent source of polysaccharides with promising antioxidant and anticancer activity.

polysaccharides, oxidative stress, endophytes, Penicillium, natural product, prostate cancer

Structure type: structural motif or average structure
Location inside paper: Fig. 5, IPS-2
Trivial name: intracellular polysaccharide (IRS-2)

Methods: 13C NMR, 1H NMR, FTIR, composition analysis, HPLC, extraction, statistical analysis, antioxidant activities, flow cytometry analysis, cell viability assay, cytotoxicity assay, fluorescent staining analysis
Biological activity: IPS-2 showed the highest ABTS+ and DPPH radical scavenging and ferric reducing activities.

NCBI Taxonomy refs (TaxIDs): 1343409
Show glycosyltransferases

NMR conditions: in D2O

¹H NMR data: present in publication

¹³C NMR data: present in publication

There is only one chemically distinct structure:

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SVG MW SMILES 3D mol Ionize